Author, as appears in the article.: Fu, Yandi; Borrull, Francesc; Fontanals, Nuria; Marce, Rosa Maria
Department: Química Analítica i Química Orgànica
URV's Author/s: Borrull Ballarín, Francesc / Fontanals Torroja, Núria / Fu, Yandi / Marcé Recasens, Rosa Maria
Keywords: Waste-water Temperature Stereoisomerism Stationary phases Separation Selectors Reversed-phase Polysaccharides Polar-organic mobile phase Phenylcarbamates Pharmaceuticals Pharmaceutical preparations Lux cellulose-1 column Hydrogen-ion concentration Fate Enantioseparation Enantiomeric analysis Chiralpak cbh column Chiral drugs Cellulose Blockers Adrenergic beta-antagonists Acetonitriles Acetonitrile Abuse 2-propanol polar-organic mobile phase lux cellulose-1 column enantioseparation chiralpak cbh column chiral drugs
Abstract: © 2020 Wiley Periodicals, Inc. Two different columns—Lux Cellulose-1 and Chiralpak CBH—were evaluated for their chiral recognition abilities for eight drugs comprising three β-blockers, one antacid, and four cathinones in polar-organic elution mode and reversed-phase elution mode, respectively. The factors that affected the enantioseparation were tested and optimized to develop a suitable chiral separation method whose LC conditions are compatible with MS detection. In polar-organic elution mode with the Lux Cellulose-1 column, methanol and acetonitrile were tested as the main components of the mobile phase. In addition, the effects of adding isopropanol as organic modifier, acidic additives (formic acid), and basic additives (diethylamine) were evaluated. In reversed-phase elution mode with the Chiralpak CBH column, the effect of type and concentration of organic modifier (isopropanol, acetonitrile, and methanol), the mobile phase pH (6.4 and 5.0), and buffer concentration (1mM-20mM ammonium acetate) were evaluated. The best enantioseparation was achieved with the Chiralpak CBH column with a mobile phase composed of 5mM ammonium acetate aqueous (pH = 6.4)/methanol (95/5, v/v) at a flow rate of 0.1 mL/min and a temperature of 30°C. Under these conditions, six of eight chiral drugs were baseline separated.
Thematic Areas: Spectroscopy Saúde coletiva Química Pharmacology & pharmacy Pharmacology Organic chemistry Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Drug discovery Ciências biológicas iii Ciências biológicas ii Ciências ambientais Chemistry, organic Chemistry, medicinal Chemistry, analytical Catalysis Biotecnología Astronomia / física Analytical chemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 08990042
Author's mail: yandi.fu@estudiants.urv.cat nuria.fontanals@urv.cat nuria.fontanals@urv.cat rosamaria.marce@urv.cat francesc.borrull@urv.cat
Author identifier: 0000-0002-7534-1964 0000-0002-7534-1964 0000-0002-5667-2899 0000-0003-2718-9336
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Chirality. 32 (6): 876-884
APA: Fu, Yandi; Borrull, Francesc; Fontanals, Nuria; Marce, Rosa Maria (2020). Comparison of polysaccharide-based and protein-based chiral liquid chromatography columns for enantioseparation of drugs. Chirality, 32(6), 876-884. DOI: 10.1002/chir.23198
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications
Repositori URV