Author, as appears in the article.: Alba-Patino, Alejandra; Russell, Steven M; Borges, Marcio; Pazos-Perez, Nicolas; Alvarez-Puebla, Ramon A; de la Rica, Roberto
Department: Química Física i Inorgànica
URV's Author/s: Alvarez Puebla, Ramon Angel / Pazos Pérez, Nicolás Carlos
Keywords: Hashtag Etiqueta «#» @uroweb @residentesaeu @infoAeu
Abstract: This journal is © The Royal Society of Chemistry. Detecting small variations in the levels of IL-6 is crucial for the early diagnosis of sepsis. To be useful in clinical decision-making, this requires detecting IL-6 rapidly in whole blood and with portable readers. Here we introduce immunosensors made of filter paper that use plasmonic nanoprobes to detect IL-6 rapidly in unprocessed blood with an unmodified smartphone. Key aspects of the biosensor fabrication were optimized in order to reduce the assay time without losing sensitivity. This included testing three bioconjugation routes for protein attachment to nanoprobes using gold nanoparticles covered with carboxylate or amine moieties, or polyvinylpyrrolidone (PVP), as starting materials, and using alternating layers of polyelectrolytes to bind the capture antibody to the paper substrate. Smartphone-based signal quantification was achieved with a custom-made app featuring a unique augmented reality guidance system that circumvents the need for smartphone attachments and automates all the steps involved in color quantification. The biosensors were able to detect IL-6 with a limit of detection of 0.1 pg mL-1 and a total assay time within 17 min. They could also detect an increase in IL-6 of only 12.5 pg mL-1 over basal levels in whole blood with 99% confidence. The high sensitivity and rapid turnaround time afforded by the optimized biosensors and the fully automated real-time densitometry app make our biosensors well suited for emergency healthcare situations such as the identification of potential sepsis cases.
Thematic Areas: Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General engineering General chemistry Engineering (miscellaneous) Engineering (all) Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Bioengineering Atomic and molecular physics, and optics
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 25160230
Author's mail: ramon.alvarez@urv.cat nicolas.pazos@urv.cat
Author identifier: 0000-0003-4770-5756 0000-0002-2326-4231
Record's date: 2024-11-02
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://pubs.rsc.org/en/content/articlelanding/2020/na/d0na00026d#!divAbstract
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Nanoscale Advances. 2 (3): 1253-1260
APA: Alba-Patino, Alejandra; Russell, Steven M; Borges, Marcio; Pazos-Perez, Nicolas; Alvarez-Puebla, Ramon A; de la Rica, Roberto (2020). Nanoparticle-based mobile biosensors for the rapid detection of sepsis biomarkers in whole blood. Nanoscale Advances, 2(3), 1253-1260. DOI: 10.1039/d0na00026d
Article's DOI: 10.1039/d0na00026d
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications