Articles producció científica> Química Física i Inorgànica

Nanoparticle-based mobile biosensors for the rapid detection of sepsis biomarkers in whole blood

  • Identification data

    Identifier: imarina:6185849
    Authors:
    Alba-Patino, AlejandraRussell, Steven MBorges, MarcioPazos-Perez, NicolasAlvarez-Puebla, Ramon Ade la Rica, Roberto
    Abstract:
    This journal is © The Royal Society of Chemistry. Detecting small variations in the levels of IL-6 is crucial for the early diagnosis of sepsis. To be useful in clinical decision-making, this requires detecting IL-6 rapidly in whole blood and with portable readers. Here we introduce immunosensors made of filter paper that use plasmonic nanoprobes to detect IL-6 rapidly in unprocessed blood with an unmodified smartphone. Key aspects of the biosensor fabrication were optimized in order to reduce the assay time without losing sensitivity. This included testing three bioconjugation routes for protein attachment to nanoprobes using gold nanoparticles covered with carboxylate or amine moieties, or polyvinylpyrrolidone (PVP), as starting materials, and using alternating layers of polyelectrolytes to bind the capture antibody to the paper substrate. Smartphone-based signal quantification was achieved with a custom-made app featuring a unique augmented reality guidance system that circumvents the need for smartphone attachments and automates all the steps involved in color quantification. The biosensors were able to detect IL-6 with a limit of detection of 0.1 pg mL-1 and a total assay time within 17 min. They could also detect an increase in IL-6 of only 12.5 pg mL-1 over basal levels in whole blood with 99% confidence. The high sensitivity and rapid turnaround time afforded by the optimized biosensors and the fully automated real-time densitometry app make our biosensors well suited for emergency healthcare situations such as the identification of potential sepsis cases.
  • Others:

    Author, as appears in the article.: Alba-Patino, Alejandra; Russell, Steven M; Borges, Marcio; Pazos-Perez, Nicolas; Alvarez-Puebla, Ramon A; de la Rica, Roberto
    Department: Química Física i Inorgànica
    URV's Author/s: Alvarez Puebla, Ramon Angel / Pazos Pérez, Nicolás Carlos
    Keywords: Hashtag Etiqueta «#» @uroweb @residentesaeu @infoAeu
    Abstract: This journal is © The Royal Society of Chemistry. Detecting small variations in the levels of IL-6 is crucial for the early diagnosis of sepsis. To be useful in clinical decision-making, this requires detecting IL-6 rapidly in whole blood and with portable readers. Here we introduce immunosensors made of filter paper that use plasmonic nanoprobes to detect IL-6 rapidly in unprocessed blood with an unmodified smartphone. Key aspects of the biosensor fabrication were optimized in order to reduce the assay time without losing sensitivity. This included testing three bioconjugation routes for protein attachment to nanoprobes using gold nanoparticles covered with carboxylate or amine moieties, or polyvinylpyrrolidone (PVP), as starting materials, and using alternating layers of polyelectrolytes to bind the capture antibody to the paper substrate. Smartphone-based signal quantification was achieved with a custom-made app featuring a unique augmented reality guidance system that circumvents the need for smartphone attachments and automates all the steps involved in color quantification. The biosensors were able to detect IL-6 with a limit of detection of 0.1 pg mL-1 and a total assay time within 17 min. They could also detect an increase in IL-6 of only 12.5 pg mL-1 over basal levels in whole blood with 99% confidence. The high sensitivity and rapid turnaround time afforded by the optimized biosensors and the fully automated real-time densitometry app make our biosensors well suited for emergency healthcare situations such as the identification of potential sepsis cases.
    Thematic Areas: Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General engineering General chemistry Engineering (miscellaneous) Engineering (all) Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Bioengineering Atomic and molecular physics, and optics
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 25160230
    Author's mail: ramon.alvarez@urv.cat nicolas.pazos@urv.cat
    Author identifier: 0000-0003-4770-5756 0000-0002-2326-4231
    Record's date: 2024-11-02
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://pubs.rsc.org/en/content/articlelanding/2020/na/d0na00026d#!divAbstract
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Nanoscale Advances. 2 (3): 1253-1260
    APA: Alba-Patino, Alejandra; Russell, Steven M; Borges, Marcio; Pazos-Perez, Nicolas; Alvarez-Puebla, Ramon A; de la Rica, Roberto (2020). Nanoparticle-based mobile biosensors for the rapid detection of sepsis biomarkers in whole blood. Nanoscale Advances, 2(3), 1253-1260. DOI: 10.1039/d0na00026d
    Article's DOI: 10.1039/d0na00026d
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2020
    Publication Type: Journal Publications
  • Keywords:

    Atomic and Molecular Physics, and Optics,Bioengineering,Chemistry (Miscellaneous),Chemistry, Multidisciplinary,Engineering (Miscellaneous),Materials Science (Miscellaneous),Materials Science, Multidisciplinary,Nanoscience & Nanotechnology
    Nanoscience & nanotechnology
    Materials science, multidisciplinary
    Materials science (miscellaneous)
    Materials science (all)
    General materials science
    General engineering
    General chemistry
    Engineering (miscellaneous)
    Engineering (all)
    Chemistry, multidisciplinary
    Chemistry (miscellaneous)
    Chemistry (all)
    Bioengineering
    Atomic and molecular physics, and optics
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