Articles producció científica> Bioquímica i Biotecnologia

The lipid composition of yeast cells modulates the response to iron deficiency

  • Identification data

    Identifier: imarina:6232237
    Authors:
    Jordá TRomero AMPerea-García ARozès NPuig S
    Abstract:
    © 2020 Elsevier B.V. Iron is a vital micronutrient for all eukaryotes because it participates as a redox cofactor in multiple metabolic pathways, including lipid biosynthesis. In response to iron deficiency, the Saccharomyces cerevisiae iron-responsive transcription factor Aft1 accumulates in the nucleus and activates a set of genes that promote iron acquisition at the cell surface. In this study, we report that yeast cells lacking the transcription factor Mga2, which promotes the expression of the iron-dependent Δ9-fatty acid desaturase Ole1, display a defect in the activation of the iron regulon during the adaptation to iron limitation. Supplementation with exogenous unsaturated fatty acids (UFAs) or OLE1 expression rescues the iron regulon activation defect of mga2Δ cells. These observations and fatty acid measurements suggest that the mga2Δ defect in iron regulon expression is due to low UFA levels. Subcellular localization studies reveal that low UFAs cause a mislocalization of Aft1 protein to the vacuole upon iron deprivation that prevents its nuclear accumulation. These results indicate that Mga2 and Ole1 are essential to maintain the UFA levels required for Aft1-dependent activation of the iron regulon in response to iron deficiency, and directly connect the biosynthesis of fatty acids to the response to iron depletion.
  • Others:

    Author, as appears in the article.: Jordá T; Romero AM; Perea-García A; Rozès N; Puig S
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Rozès, Nicolas Andre Louis
    Keywords: Yeast Saccharomyces cerevisiae Ole1 Mga2 Iron deficiency Fatty acids Aft1 saccharomyces cerevisiae ole1 mga2 iron deficiency fatty acids aft1
    Abstract: © 2020 Elsevier B.V. Iron is a vital micronutrient for all eukaryotes because it participates as a redox cofactor in multiple metabolic pathways, including lipid biosynthesis. In response to iron deficiency, the Saccharomyces cerevisiae iron-responsive transcription factor Aft1 accumulates in the nucleus and activates a set of genes that promote iron acquisition at the cell surface. In this study, we report that yeast cells lacking the transcription factor Mga2, which promotes the expression of the iron-dependent Δ9-fatty acid desaturase Ole1, display a defect in the activation of the iron regulon during the adaptation to iron limitation. Supplementation with exogenous unsaturated fatty acids (UFAs) or OLE1 expression rescues the iron regulon activation defect of mga2Δ cells. These observations and fatty acid measurements suggest that the mga2Δ defect in iron regulon expression is due to low UFA levels. Subcellular localization studies reveal that low UFAs cause a mislocalization of Aft1 protein to the vacuole upon iron deprivation that prevents its nuclear accumulation. These results indicate that Mga2 and Ole1 are essential to maintain the UFA levels required for Aft1-dependent activation of the iron regulon in response to iron deficiency, and directly connect the biosynthesis of fatty acids to the response to iron depletion.
    Thematic Areas: Química Molecular biology Medicina ii Medicina i Linguística e literatura Interdisciplinar Farmacia Engenharias iii Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biotecnología Biophysics Biodiversidade Biochemistry & molecular biology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1388-1981
    Author's mail: nicolasrozes@urv.cat
    Author identifier: 0000-0001-9718-3429
    Record's date: 2023-02-22
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1388198120300998?via%3Dihub
    Papper original source: Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids. 1865 (8): 158707-
    APA: Jordá T; Romero AM; Perea-García A; Rozès N; Puig S (2020). The lipid composition of yeast cells modulates the response to iron deficiency. Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids, 1865(8), 158707-. DOI: 10.1016/j.bbalip.2020.158707
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.bbalip.2020.158707
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2020
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry & Molecular Biology,Biophysics,Cell Biology,Molecular Biology
    Yeast
    Saccharomyces cerevisiae
    Ole1
    Mga2
    Iron deficiency
    Fatty acids
    Aft1
    saccharomyces cerevisiae
    ole1
    mga2
    iron deficiency
    fatty acids
    aft1
    Química
    Molecular biology
    Medicina ii
    Medicina i
    Linguística e literatura
    Interdisciplinar
    Farmacia
    Engenharias iii
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Cell biology
    Biotecnología
    Biophysics
    Biodiversidade
    Biochemistry & molecular biology
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