Author, as appears in the article.: Tomas J; Lanuza M; Santafé M; Fenoll-Brunet M; Garcia N
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Fenoll Brunet, Maria Rosa / Garcia Sancho, Maria de les Neus / Lanuza Escolano, María Angel / Santafé Martínez, Manuel / Tomás Ferré, José Maria
Keywords: Visualization Neurotoxins Neuronal plasticity Neuromuscular junction Nerve terminal sprouting Mouse neuromuscular-junctions Morphology Mice Locomotor-activity Growth Exercise End-plates Auris longus muscle Adult-rat Acetylcholine-release
Abstract: Spontaneous sproutings can be observed in end plates from normal adult vertebrate muscles and motor end plates develop increased growth signs and sprouts when target muscle cells become less active or paralysed. Nevertheless, very little is known about where in the motor nerve terminal arborization spontaneous and experimentally induced sprouts originate, their similarities and differences and also about their final maturation or elimination. In this study we investigate the topological properties of both spontaneous and alpha-bungarotoxin-induced sprouts (during different periods of intoxication and after recovery) along the motor nerve terminal branches of the Levator auris longus muscle of Swiss mice (between 48169 day old). Muscles were processed for immunocytochemistry to simultaneously detect postsynaptic AChRs and axons. This procedure permits us to make an accurate identification of the fine sprouts and a morphometric study of the presynaptic branching pattern profile in control muscles, during the toxin action and after recovery from paralysis. The results show that in normal muscles, the initial and trunk segments (those between branch points) of the terminal arborization sprouted proportionally more branches when taking their relative lengths into account than the distal free-end segments. In contrast, every micrometer of alpha-bungarotoxin-treated muscles throughout the full terminal arborization have the same probability of generating a sprout. Moreover, the toxin-induced sprouts can consolidate as new branches once recovered from the paralysis without changing the total length of the nerve terminal arborization.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Pathology and forensic medicine Pathology Odontología Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Histology General medicine Farmacia Engenharias iv Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Cell biology Biotecnología Biodiversidade
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 02133911
Author's mail: manuel.santafe@urv.cat mariaangel.lanuza@urv.cat mariarosa.fenoll@urv.cat josepmaria.tomas@urv.cat
Author identifier: 0000-0002-5462-5108 0000-0003-4795-4103 0000-0002-5921-7277 0000-0002-0406-0006
Record's date: 2024-02-03
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.hh.um.es/Abstracts/Vol_15/15_1/15_1_6.htm
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Histology And Histopathology. 15 (1): 43-52
APA: Tomas J; Lanuza M; Santafé M; Fenoll-Brunet M; Garcia N (2000). Topological differences along mammalian motor nerve terminals for spontaneous and alpha-bungarotoxin-induced sprouting. Histology And Histopathology, 15(1), 43-52. DOI: 10.14670/HH-15.43
Article's DOI: 10.14670/HH-15.43
Entity: Universitat Rovira i Virgili
Journal publication year: 2000
Publication Type: Journal Publications
Repositori URV