Repositori URV

Author, as appears in the article.: Lluís L; Taltavull N; Muñoz-Cortés M; Sánchez-Martos V; Romeu M; Giralt M; Molinar-Toribio E; Torres J; Pérez-Jiménez J; Pazos M; Méndez L; Gallardo J; Medina I; Nogués M

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Giralt Batista, Montserrat / Muñoz Cortés, Mónica / Nogués Llort, Maria Rosa / Romeu Ferran, Marta / Sánchez Martos, Vanessa / Taltavull Anglada, Núria

Keywords: Triglycerides Triacylglycerols Supplementation Superoxide-dismutase Superoxide dismutase Rats, wistar Rats Oxidized low density lipoprotein Oxidative stress Omega-3 pufa Omega-3 polyunsaturated fatty acids (pufa) Metabolic syndrome Malondialdehyde Liver Lipoproteins, ldl Lipid peroxidation Insulin resistance Increases Hypertension Glycated hemoglobin a Glycated hemoglobin Glutathione reductase Glutathione peroxidase Fish-oil Fish oils Female Erythrocytes Eicosapentaenoic acid (epa) Eicosapentaenoic acid Docosahexaenoic acids Docosahexaenoic acid (dha) Diet Controlled-trial Cholesterol, ldl Cholesterol, hdl Catalase Cardiovascular diseases Cardiovascular disease risk C-reactive protein Apolipoproteins Antioxidant status Animals

Abstract: Background: High consumption of fish carries a lower risk of cardiovascular disease as a consequence of dietary omega-3 long chain polyunsaturated fatty acid (n-3 PUFA; especially EPA and DHA) content. A controversy exists about the component/s responsible of these beneficial effects and, in consequence, which is the best proportion between both fatty acids. We sought to determine, in healthy Wistar rats, the proportions of EPA and DHA that would induce beneficial effects on biomarkers of oxidative stress, and cardiovascular disease risk. Methods. Female Wistar rats were fed for 13 weeks with 5 different dietary supplements of oils; 3 derived from fish (EPA/DHA ratios of 1:1, 2:1, 1:2) plus soybean and linseed as controls. The activities of major antioxidant enzymes (SOD, CAT, GPX, and GR) were determined in erythrocytes and liver, and the ORAC test was used to determine the antioxidant capacity in plasma. Also measured were: C reactive protein (CRP), endothelial dysfunction (sVCAM and sICAM), prothrombotic activity (PAI-1), lipid profile (triglycerides, cholesterol, HDLc, LDLc, Apo-A1, and Apo-B100), glycated haemoglobin and lipid peroxidation (LDL-ox and MDA values). Results: After three months of nutritional intervention, we observed statistically significant differences in the ApoB100/ApoA1 ratio, glycated haemoglobin, VCAM-1, SOD and GPx in erythrocytes, ORAC values and LDL-ox. Supplementation with fish oil derived omega-3 PUFA increased VCAM-1, LDL-ox and plasma antioxidant capacity (ORAC). Conversely, the ApoB100/ApoA1 ratio and percentage glycated haemoglobin decreased. Conclusions: Our results showed that a diet of a 1:1 ratio of EPA/DHA improved many of the oxidative stress parameters (SOD and GPx in erythrocytes), plasma antioxidant capacity (ORAC) and cardiovascular risk factors (glycated haemoglobin) relative to the other diets. © 2013 Lluís et al.; licensee BioMed Central Ltd.

Thematic Areas: Saúde coletiva Química Odontología Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Ensino Enfermagem Endocrinology, diabetes and metabolism Endocrinology Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade Biochemistry (medical) Biochemistry & molecular biology

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1476511X

Author's mail: monica.munoz@urv.cat nuria.taltavull@urv.cat montse.giralt@urv.cat vanessa.sanchez@urv.cat vanessa.sanchez@urv.cat marta.romeu@urv.cat

Author identifier: 0000-0002-3340-2650 0000-0002-7073-577X 0000-0002-2131-1858

Record's date: 2025-01-08

Paper version: info:eu-repo/semantics/publishedVersion

Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/

Paper original source: Lipids In Health And Disease. 12 (1): 140-

APA: Lluís L; Taltavull N; Muñoz-Cortés M; Sánchez-Martos V; Romeu M; Giralt M; Molinar-Toribio E; Torres J; Pérez-Jiménez J; Pazos M; Méndez L; Gallardo J (2013). Protective effect of the omega-3 polyunsaturated fatty acids: Eicosapentaenoic acid/Docosahexaenoic acid 1:1 ratio on cardiovascular disease risk markers in rats. Lipids In Health And Disease, 12(1), 140-. DOI: 10.1186/1476-511X-12-140

Entity: Universitat Rovira i Virgili

Journal publication year: 2013

Publication Type: Journal Publications