Articles producció científica> Ciències Mèdiques Bàsiques

Protective effect of the omega-3 polyunsaturated fatty acids: Eicosapentaenoic acid/Docosahexaenoic acid 1:1 ratio on cardiovascular disease risk markers in rats

  • Identification data

    Identifier: imarina:6387498
    Handle: https://hdl.handle.net/20.500.11797/imarina6387498
    Authors:
    Lluís LTaltavull NMuñoz-Cortés MSánchez-Martos VRomeu MGiralt MMolinar-Toribio ETorres JPérez-Jiménez JPazos MMéndez LGallardo JMedina INogués M
    Abstract:
    Background: High consumption of fish carries a lower risk of cardiovascular disease as a consequence of dietary omega-3 long chain polyunsaturated fatty acid (n-3 PUFA; especially EPA and DHA) content. A controversy exists about the component/s responsible of these beneficial effects and, in consequence, which is the best proportion between both fatty acids. We sought to determine, in healthy Wistar rats, the proportions of EPA and DHA that would induce beneficial effects on biomarkers of oxidative stress, and cardiovascular disease risk. Methods. Female Wistar rats were fed for 13 weeks with 5 different dietary supplements of oils; 3 derived from fish (EPA/DHA ratios of 1:1, 2:1, 1:2) plus soybean and linseed as controls. The activities of major antioxidant enzymes (SOD, CAT, GPX, and GR) were determined in erythrocytes and liver, and the ORAC test was used to determine the antioxidant capacity in plasma. Also measured were: C reactive protein (CRP), endothelial dysfunction (sVCAM and sICAM), prothrombotic activity (PAI-1), lipid profile (triglycerides, cholesterol, HDLc, LDLc, Apo-A1, and Apo-B100), glycated haemoglobin and lipid peroxidation (LDL-ox and MDA values). Results: After three months of nutritional intervention, we observed statistically significant differences in the ApoB100/ApoA1 ratio, glycated haemoglobin, VCAM-1, SOD and GPx in erythrocytes, ORAC values and LDL-ox. Supplementation with fish oil derived omega-3 PUFA increased VCAM-1, LDL-ox and plasma antioxidant capacity (ORAC). Conversely, the ApoB100/ApoA1 ratio and percentage glycated haemoglobin decreased. Conclusions: Our results showed that a diet of a 1:1 ratio of EPA/DHA improved many of the oxidative stress parameters (SOD and GPx in erythrocytes), plasma antioxidant capacity (ORAC) and cardio

  • Others:

    Author, as appears in the article.: Lluís L; Taltavull N; Muñoz-Cortés M; Sánchez-Martos V; Romeu M; Giralt M; Molinar-Toribio E; Torres J; Pérez-Jiménez J; Pazos M; Méndez L; Gallardo J; Medina I; Nogués M
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Giralt Batista, Montserrat / Muñoz Cortés, Mónica / Nogués Llort, Maria Rosa / Romeu Ferran, Marta / Sánchez Martos, Vanessa / Taltavull Anglada, Núria
    Keywords: Triacylglycerols Supplementation Superoxide-dismutase Oxidative stress Omega-3 pufa Omega-3 polyunsaturated fatty acids (pufa) Metabolic syndrome Liver Insulin resistance Increases Hypertension Fish-oil Fish oils Eicosapentaenoic acid (epa) Docosahexaenoic acid (dha) Controlled-trial Cardiovascular disease risk Antioxidant status
    Abstract: Background: High consumption of fish carries a lower risk of cardiovascular disease as a consequence of dietary omega-3 long chain polyunsaturated fatty acid (n-3 PUFA; especially EPA and DHA) content. A controversy exists about the component/s responsible of these beneficial effects and, in consequence, which is the best proportion between both fatty acids. We sought to determine, in healthy Wistar rats, the proportions of EPA and DHA that would induce beneficial effects on biomarkers of oxidative stress, and cardiovascular disease risk. Methods. Female Wistar rats were fed for 13 weeks with 5 different dietary supplements of oils; 3 derived from fish (EPA/DHA ratios of 1:1, 2:1, 1:2) plus soybean and linseed as controls. The activities of major antioxidant enzymes (SOD, CAT, GPX, and GR) were determined in erythrocytes and liver, and the ORAC test was used to determine the antioxidant capacity in plasma. Also measured were: C reactive protein (CRP), endothelial dysfunction (sVCAM and sICAM), prothrombotic activity (PAI-1), lipid profile (triglycerides, cholesterol, HDLc, LDLc, Apo-A1, and Apo-B100), glycated haemoglobin and lipid peroxidation (LDL-ox and MDA values). Results: After three months of nutritional intervention, we observed statistically significant differences in the ApoB100/ApoA1 ratio, glycated haemoglobin, VCAM-1, SOD and GPx in erythrocytes, ORAC values and LDL-ox. Supplementation with fish oil derived omega-3 PUFA increased VCAM-1, LDL-ox and plasma antioxidant capacity (ORAC). Conversely, the ApoB100/ApoA1 ratio and percentage glycated haemoglobin decreased. Conclusions: Our results showed that a diet of a 1:1 ratio of EPA/DHA improved many of the oxidative stress parameters (SOD and GPx in erythrocytes), plasma antioxidant capacity (ORAC) and cardiovascular risk factors (glycated haemoglobin) relative to the other diets. © 2013 Lluís et al.; licensee BioMed Central Ltd.
    Thematic Areas: Saúde coletiva Química Odontología Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Ensino Enfermagem Endocrinology, diabetes and metabolism Endocrinology Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade Biochemistry (medical) Biochemistry & molecular biology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1476511X
    Author's mail: monica.munoz@urv.cat nuria.taltavull@urv.cat vanessa.sanchez@urv.cat mariarosa.nogues@urv.cat marta.romeu@urv.cat vanessa.sanchez@urv.cat montse.giralt@urv.cat
    Author identifier: 0000-0002-3340-2650 0000-0003-4626-5672 0000-0002-2131-1858 0000-0002-7073-577X
    Record's date: 2023-02-26
    Papper version: info:eu-repo/semantics/publishedVersion
    Papper original source: Lipids In Health And Disease. 12 (1): 140-
    APA: Lluís L; Taltavull N; Muñoz-Cortés M; Sánchez-Martos V; Romeu M; Giralt M; Molinar-Toribio E; Torres J; Pérez-Jiménez J; Pazos M; Méndez L; Gallardo J (2013). Protective effect of the omega-3 polyunsaturated fatty acids: Eicosapentaenoic acid/Docosahexaenoic acid 1:1 ratio on cardiovascular disease risk markers in rats. Lipids In Health And Disease, 12(1), 140-. DOI: 10.1186/1476-511X-12-140
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2013
    Publication Type: Journal Publications

  • Keywords:

    Biochemistry & Molecular Biology,Biochemistry (Medical),Clinical Biochemistry,Endocrinology,Endocrinology, Diabetes and Metabolism,Nutrition & Dietetics
    Triacylglycerols
    Supplementation
    Superoxide-dismutase
    Oxidative stress
    Omega-3 pufa
    Omega-3 polyunsaturated fatty acids (pufa)
    Metabolic syndrome
    Liver
    Insulin resistance
    Increases
    Hypertension
    Fish-oil
    Fish oils
    Eicosapentaenoic acid (epa)
    Docosahexaenoic acid (dha)
    Controlled-trial
    Cardiovascular disease risk
    Antioxidant status
    Saúde coletiva
    Química
    Odontología
    Nutrition & dietetics
    Nutrição
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Farmacia
    Ensino
    Enfermagem
    Endocrinology, diabetes and metabolism
    Endocrinology
    Educação física
    Clinical biochemistry
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciência de alimentos
    Biotecnología
    Biodiversidade
    Biochemistry (medical)
    Biochemistry & molecular biology

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