Author, as appears in the article.: Irinyi L; Serena C; Garcia-Hermoso D; Arabatzis M; Desnos-Ollivier M; Vu D; Cardinali G; Arthur I; Normand AC; Giraldo A; Da Cunha KC; Sandoval-Denis M; Hendrickx M; Nishikaku AS; De Azevedo Melo AS; Merseguel KB; Khan A; Parente Rocha JA; Sampaio P; Da Silva Briones MR; E Ferreira RC; De Medeiros Muniz M; Castañón-Olivares LR; Estrada-Barcenas D; Cassagne C; Mary C; Duan SY; Kong F; Sun AY; Zeng X; Zhao Z; Gantois N; Botterel F; Robbertse B; Schoch C; Gams W; Ellis D; Halliday C; Chen S; Sorrell TC
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Cano Lira, José Francisco / Guarro Artigas, Josep / SANDOVAL DENIS, MARCELO PATRICIO / Serena Perelló, Carolina
Keywords: Trichophyton-mentagrophytes Transcribed spacer Solani species complex Sequence-based identification Scedosporium-apiospermum Ribosomal dna Reference its database Molecular taxonomy Its region Intraspecies/interspecies genetic diversity Intraspecies Interspecies genetic diversity Genetic diversity Fungal identification Dna barcoding Cryptococcus-neoformans Candida-lusitaniae its region intraspecies/interspecies genetic diversity fungal identification dna barcoding
Abstract: © The Author 2015. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. Human and animal fungal pathogens are a growing threat worldwide leading to emerging infections and creating new risks for established ones. There is a growing need for a rapid and accurate identification of pathogens to enable early diagnosis and targeted antifungal therapy. Morphological and biochemical identification methods are time-consuming and require trained experts. Alternatively, molecular methods, such as DNA barcoding, a powerful and easy tool for rapid monophasic identification, offer a practical approach for species identification and less demanding in terms of taxonomical expertise. However, its wide-spread use is still limited by a lack of quality-controlled reference databases and the evolving recognition and definition of new fungal species/complexes. An international consortium of medical mycology laboratories was formed aiming to establish a quality controlled ITS database under the umbrella of the ISHAM working group on DNA barcoding of human and animal pathogenic fungi. A new database, containing 2800 ITS sequences representing 421 fungal species, providing the medical community with a freely accessible tool at http://www.isham.org/ and http://its.mycologylab.org/ to rapidly and reliably identify most agents of mycoses, was established. The generated sequences included in the new database were used to evaluate the variation and overall utility of the ITS region for the identification of pathogenic fungi at intra-and interspecies level. The average intraspecies variation ranged from 0 to 2.25%. This highlighted selected pathogenic fungal species, such as the dermatophytes and emerging yeast, for which additional molecular methods/genetic markers are required for their reliable identification from clinical and veterinary specimens.
Thematic Areas: Zootecnia / recursos pesqueiros Veterinary sciences Veterinary (miscellaneous) Saúde coletiva Química Odontología Mycology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Infectious diseases Geografía General veterinary Farmacia Engenharias iii Engenharias ii Engenharias i Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 13693786
Author's mail: carolina.serena@urv.cat jose.cano@urv.cat
Author identifier: 0000-0003-4495-4394
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Medical Mycology. 53 (4): 313-337
APA: Irinyi L; Serena C; Garcia-Hermoso D; Arabatzis M; Desnos-Ollivier M; Vu D; Cardinali G; Arthur I; Normand AC; Giraldo A; Da Cunha KC; Sandoval-Denis (2015). International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database - The quality controlled standard tool for routine identification of human and animal pathogenic fungi. Medical Mycology, 53(4), 313-337. DOI: 10.1093/mmy/myv008
Entity: Universitat Rovira i Virgili
Journal publication year: 2015
Publication Type: Journal Publications
Repositori URV