Articles producció científica> Ciències Mèdiques Bàsiques

International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database - The quality controlled standard tool for routine identification of human and animal pathogenic fungi

  • Identification data

    Identifier: imarina:6388203
    Authors:
    Irinyi, LaszloSerena, CarolinaGarcia-Hermoso, DeaArabatzis, MichaelDesnos-Ollivier, MarieVu, DuongCardinali, GianluigiArthur, IanNormand, Anne-CecileGiraldo, AlejandraCassia da Cunha, KeithSandoval-Denis, MarceloHendrickx, MarijkeNishikaku, Angela Satiede Azevedo Melo, Analy SallesMerseguel, Karina BellinghausenKhan, AzizaParente Rocha, Juliana AlvesSampaio, Paulada Silva Briones, Marcelo RibeiroCarmona e Ferreira, Renatade Medeiros Muniz, MauroCastanon-Olivares, Laura RosioEstrada-Barcenas, DanielCassagne, CaroleMary, CharlesDuan, Shu YaoKong, FanrongSun, Annie YingZeng, XianyuZhao, ZuotaoGantois, NausicaaBotterel, FrancoiseRobbertse, BarbaraSchoch, ConradGams, WalterEllis, DavidHalliday, CatrionaChen, SharonSorrell, Tania CPiarroux, RenaudColombo, Arnaldo LPais, Celiade Hoog, SybrenZancope-Oliveira, Rosely MariaTaylor, Maria LuciaToriello, Conchitade Almeida Soares, Celia MariaDelhaes, LaurenceStubbe, DirkDromer, FrancoiseRanque, StephaneGuarro, JosepCano-Lira, Jose FRobert, VincentVelegraki, AristeaMeyer, Wieland
    Abstract:
    © The Author 2015. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. Human and animal fungal pathogens are a growing threat worldwide leading to emerging infections and creating new risks for established ones. There is a growing need for a rapid and accurate identification of pathogens to enable early diagnosis and targeted antifungal therapy. Morphological and biochemical identification methods are time-consuming and require trained experts. Alternatively, molecular methods, such as DNA barcoding, a powerful and easy tool for rapid monophasic identification, offer a practical approach for species identification and less demanding in terms of taxonomical expertise. However, its wide-spread use is still limited by a lack of quality-controlled reference databases and the evolving recognition and definition of new fungal species/complexes. An international consortium of medical mycology laboratories was formed aiming to establish a quality controlled ITS database under the umbrella of the ISHAM working group on DNA barcoding of human and animal pathogenic fungi. A new database, containing 2800 ITS sequences representing 421 fungal species, providing the medical community with a freely accessible tool at http://www.isham.org/ and http://its.mycologylab.org/ to rapidly and reliably identify most agents of mycoses, was established. The generated sequences included in the new database were used to evaluate the variation and overall utility of the ITS region for the identification of pathogenic fungi at intra-and interspecies level. The average intraspecies variation ranged from 0 to 2.25%. This highlighted selected pathogenic fungal species, such as the dermatophytes and emerging yeast, for which additional molecular met
  • Others:

    Author, as appears in the article.: Irinyi, Laszlo; Serena, Carolina; Garcia-Hermoso, Dea; Arabatzis, Michael; Desnos-Ollivier, Marie; Vu, Duong; Cardinali, Gianluigi; Arthur, Ian; Normand, Anne-Cecile; Giraldo, Alejandra; Cassia da Cunha, Keith; Sandoval-Denis, Marcelo; Hendrickx, Marijke; Nishikaku, Angela Satie; de Azevedo Melo, Analy Salles; Merseguel, Karina Bellinghausen; Khan, Aziza; Parente Rocha, Juliana Alves; Sampaio, Paula; da Silva Briones, Marcelo Ribeiro; Carmona e Ferreira, Renata; de Medeiros Muniz, Mauro; Castanon-Olivares, Laura Rosio; Estrada-Barcenas, Daniel; Cassagne, Carole; Mary, Charles; Duan, Shu Yao; Kong, Fanrong; Sun, Annie Ying; Zeng, Xianyu; Zhao, Zuotao; Gantois, Nausicaa; Botterel, Francoise; Robbertse, Barbara; Schoch, Conrad; Gams, Walter; Ellis, David; Halliday, Catriona; Chen, Sharon; Sorrell, Tania C; Piarroux, Renaud; Colombo, Arnaldo L; Pais, Celia; de Hoog, Sybren; Zancope-Oliveira, Rosely Maria; Taylor, Maria Lucia; Toriello, Conchita; de Almeida Soares, Celia Maria; Delhaes, Laurence; Stubbe, Dirk; Dromer, Francoise; Ranque, Stephane; Guarro, Josep; Cano-Lira, Jose F; Robert, Vincent; Velegraki, Aristea; Meyer, Wieland
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Cano Lira, José Francisco / Guarro Artigas, Josep / SANDOVAL DENIS, MARCELO PATRICIO / Serena Perelló, Carolina
    Keywords: Trichophyton-mentagrophytes Transcribed spacer Solani species complex Sequence-based identification Scedosporium-apiospermum Ribosomal dna Reference its database Molecular taxonomy Its region Intraspecies/interspecies genetic diversity Intraspecies Interspecies genetic diversity Genetic diversity Fungal identification Dna barcoding Cryptococcus-neoformans Candida-lusitaniae its region intraspecies/interspecies genetic diversity fungal identification dna barcoding
    Abstract: © The Author 2015. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. Human and animal fungal pathogens are a growing threat worldwide leading to emerging infections and creating new risks for established ones. There is a growing need for a rapid and accurate identification of pathogens to enable early diagnosis and targeted antifungal therapy. Morphological and biochemical identification methods are time-consuming and require trained experts. Alternatively, molecular methods, such as DNA barcoding, a powerful and easy tool for rapid monophasic identification, offer a practical approach for species identification and less demanding in terms of taxonomical expertise. However, its wide-spread use is still limited by a lack of quality-controlled reference databases and the evolving recognition and definition of new fungal species/complexes. An international consortium of medical mycology laboratories was formed aiming to establish a quality controlled ITS database under the umbrella of the ISHAM working group on DNA barcoding of human and animal pathogenic fungi. A new database, containing 2800 ITS sequences representing 421 fungal species, providing the medical community with a freely accessible tool at http://www.isham.org/ and http://its.mycologylab.org/ to rapidly and reliably identify most agents of mycoses, was established. The generated sequences included in the new database were used to evaluate the variation and overall utility of the ITS region for the identification of pathogenic fungi at intra-and interspecies level. The average intraspecies variation ranged from 0 to 2.25%. This highlighted selected pathogenic fungal species, such as the dermatophytes and emerging yeast, for which additional molecular methods/genetic markers are required for their reliable identification from clinical and veterinary specimens.
    Thematic Areas: Zootecnia / recursos pesqueiros Veterinary sciences Veterinary (miscellaneous) Saúde coletiva Química Odontología Mycology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Infectious diseases Geografía General veterinary Farmacia Engenharias iii Engenharias ii Engenharias i Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 13693786
    Author's mail: carolina.serena@urv.cat jose.cano@urv.cat
    Author identifier: 0000-0003-4495-4394
    Record's date: 2025-02-08
    Paper version: info:eu-repo/semantics/acceptedVersion
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Paper original source: Medical Mycology. 53 (4): 313-337
    APA: Irinyi, Laszlo; Serena, Carolina; Garcia-Hermoso, Dea; Arabatzis, Michael; Desnos-Ollivier, Marie; Vu, Duong; Cardinali, Gianluigi; Arthur, Ian; Norma (2015). International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database - The quality controlled standard tool for routine identification of human and animal pathogenic fungi. Medical Mycology, 53(4), 313-337. DOI: 10.1093/mmy/myv008
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2015
    Publication Type: Journal Publications
  • Keywords:

    Infectious Diseases,Medicine (Miscellaneous),Mycology,Veterinary (Miscellaneous),Veterinary Sciences
    Trichophyton-mentagrophytes
    Transcribed spacer
    Solani species complex
    Sequence-based identification
    Scedosporium-apiospermum
    Ribosomal dna
    Reference its database
    Molecular taxonomy
    Its region
    Intraspecies/interspecies genetic diversity
    Intraspecies
    Interspecies genetic diversity
    Genetic diversity
    Fungal identification
    Dna barcoding
    Cryptococcus-neoformans
    Candida-lusitaniae
    its region
    intraspecies/interspecies genetic diversity
    fungal identification
    dna barcoding
    Zootecnia / recursos pesqueiros
    Veterinary sciences
    Veterinary (miscellaneous)
    Saúde coletiva
    Química
    Odontología
    Mycology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Infectious diseases
    Geografía
    General veterinary
    Farmacia
    Engenharias iii
    Engenharias ii
    Engenharias i
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Biotecnología
    Biodiversidade
    Astronomia / física
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