Author, as appears in the article.: Santafe M; Priego M; Obis T; Garcia N; Tomàs M; Lanuza M; Tomàs J
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Garcia Sancho, Maria de les Neus / Lanuza Escolano, María Angel / PRIEGO LUQUE, MERCEDES / Santafé Martínez, Manuel / Tomás Ferré, José Maria / Tomas Marginet, Marta
Keywords: Presynaptic receptors Neurotransmission Neuromuscular junction Mouse Motor nerve terminal Cholinergic synapses neurotransmission neuromuscular junction mouse motor nerve terminal cholinergic synapses
Abstract: © 2015 Federation of European Neuroscience Societies and John Wiley & Sons Ltd. Adenosine receptors (ARs) are present in the motor terminals at the mouse neuromuscular junction. ARs and the presynaptic muscarinic acetylcholine receptors (mAChRs) share the functional control of the neuromuscular junction. We analysed their mutual interaction in transmitter release modulation. In electrophysiological experiments with unaltered synaptic transmission (muscles paralysed by blocking the voltage-dependent sodium channel of the muscle cells with μ-conotoxin GIIIB), we found that: (i) a collaborative action between different AR subtypes reduced synaptic depression at a moderate activity level (40 Hz); (ii) at high activity levels (100 Hz), endogenous adenosine production in the synaptic cleft was sufficient to reduce depression through A1-type receptors (A1Rs) and A2A-type receptors (A2ARs); (iii) when the non-metabolizable 2-chloroadenosine (CADO) agonist was used, both the quantal content and depression were reduced; (iv) the protective effect of CADO on depression was mediated by A1Rs, whereas A2ARs seemed to modulate A1Rs; (v) ARs and mAChRs absolutely depended upon each other for the modulation of evoked and spontaneous acetylcholine release in basal conditions and in experimental conditions with CADO stimulation; (vi) the purinergic and muscarinic mechanisms cooperated in the control of depression by sharing a common pathway although the purinergic control was more powerful than the muscarinic control; and (vii) the imbalance of the ARs created by using subtype-selective and non-selective inhibitory and stimulatory agents uncoupled protein kinase C from evoked transmitter release. In summary, ARs (A1Rs, A2ARs) and mAChRs (M1, M2) cooperated in the control of activity-dependent synaptic depression and may share a common protein kinase C pathway.
Thematic Areas: Química Psicología Odontología Neurosciences Neuroscience (miscellaneous) Neuroscience (all) Medicina iii Medicina ii Medicina i Interdisciplinar General neuroscience General medicine Farmacia Engenharias iv Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0953816X
Author's mail: marta.tomas@urv.cat josepmaria.tomas@urv.cat mariaangel.lanuza@urv.cat manuel.santafe@urv.cat
Author identifier: 0000-0002-4151-1697 0000-0002-0406-0006 0000-0003-4795-4103 0000-0002-5462-5108
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://onlinelibrary.wiley.com/doi/epdf/10.1111/ejn.12922
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: European Journal Of Neuroscience. 42 (2): 1775-1787
APA: Santafe M; Priego M; Obis T; Garcia N; Tomàs M; Lanuza M; Tomàs J (2015). Adenosine receptors and muscarinic receptors cooperate in acetylcholine release modulation in the neuromuscular synapse. European Journal Of Neuroscience, 42(2), 1775-1787. DOI: 10.1111/ejn.12922
Article's DOI: 10.1111/ejn.12922
Entity: Universitat Rovira i Virgili
Journal publication year: 2015
Publication Type: Journal Publications
Repositori URV