Articles producció científica> Ciències Mèdiques Bàsiques

Species diversity of Aspergillus section Versicolores in clinical samples and antifungal susceptibility

  • Identification data

    Identifier: imarina:6388886
    Authors:
    Siqueira JSutton DGarcía DGené JThomson PWiederhold NGuarro J
    Abstract:
    © 2016 British Mycological Society Aspergillus section Versicolores includes species of clinical relevance and many others that have been poorly studied but are occasionally found in clinical samples. The aim of this study was to investigate, using a multilocus phylogenetic approach, the spectrum of species of the section Versicolores and to determine their in vitro antifungal susceptibility. The study was based on a set of 77 clinical isolates from different USA medical centres, which had been previously identified as belonging to this section. The genetic markers used were internal transcribed spacer (ITS), β-tubulin (BenA), calmodulin (CaM), and RNA polymerase II second largest subunit (RPB2), and the drugs tested, following the CLSI guidelines, were amphotericin B (AMB), itraconazole, posaconazole, voriconazole, anidulafungin, caspofungin, micafungin, terbinafine (TBF), and flucytosine (5FC). The most frequent species were Aspergillus sydowii (26 %), Aspergillus creber (22 %), and Aspergillus amoenus (18.2 %), followed by Aspergillus protuberus (13 %), Aspergillus jensenii (10.4 %), and Aspergillus tabacinus (5.2 %); while Aspergillus cvjetkovicii, Aspergillus fructus, Aspergillus puulaauensis, and Aspergillus versicolor were represented by only one isolate each (1.3 %). This is the first time that A. jensenii and A. puulaauensis have been reported from clinical samples. Considering the high number of isolates identified as belonging to this fungal group in this study, its clinical relevance should not be overlooked. Aspergillus versicolor, traditionally considered one of the most common species in this section in a clinical setting, was only rarely recovered in our study. The in vitro antifungal results showed that echinocandins and TBF were the most potent drugs,
  • Others:

    Author, as appears in the article.: Siqueira J; Sutton D; García D; Gené J; Thomson P; Wiederhold N; Guarro J
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: García Sánchez, Dania / Gené Díaz, Josepa / Guarro Artigas, Josep / ZEN SIQUEIRA, JOAO PAULO
    Keywords: Taxonomy Sydowii Penicillium Onychomycosis Multilocus phylogeny Metabolites Infection Identification Endolichenic fungus Aspergilli Ascomycetes Antufungal activity Amphotericin-b Agents multilocus phylogeny aspergilli ascomycetes antufungal activity
    Abstract: © 2016 British Mycological Society Aspergillus section Versicolores includes species of clinical relevance and many others that have been poorly studied but are occasionally found in clinical samples. The aim of this study was to investigate, using a multilocus phylogenetic approach, the spectrum of species of the section Versicolores and to determine their in vitro antifungal susceptibility. The study was based on a set of 77 clinical isolates from different USA medical centres, which had been previously identified as belonging to this section. The genetic markers used were internal transcribed spacer (ITS), β-tubulin (BenA), calmodulin (CaM), and RNA polymerase II second largest subunit (RPB2), and the drugs tested, following the CLSI guidelines, were amphotericin B (AMB), itraconazole, posaconazole, voriconazole, anidulafungin, caspofungin, micafungin, terbinafine (TBF), and flucytosine (5FC). The most frequent species were Aspergillus sydowii (26 %), Aspergillus creber (22 %), and Aspergillus amoenus (18.2 %), followed by Aspergillus protuberus (13 %), Aspergillus jensenii (10.4 %), and Aspergillus tabacinus (5.2 %); while Aspergillus cvjetkovicii, Aspergillus fructus, Aspergillus puulaauensis, and Aspergillus versicolor were represented by only one isolate each (1.3 %). This is the first time that A. jensenii and A. puulaauensis have been reported from clinical samples. Considering the high number of isolates identified as belonging to this fungal group in this study, its clinical relevance should not be overlooked. Aspergillus versicolor, traditionally considered one of the most common species in this section in a clinical setting, was only rarely recovered in our study. The in vitro antifungal results showed that echinocandins and TBF were the most potent drugs, the azoles showed variable results, AMB was poorly active, and 5FC was the less active.
    Research group: Unitat de Micologia i Microbiologia Ambiental
    Thematic Areas: Zootecnia / recursos pesqueiros Química Plant science Mycology Medicina veterinaria Medicina ii Medicina i Interdisciplinar Infectious diseases Genetics Farmacia Engenharias iv Economia Ecology, evolution, behavior and systematics Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 18786146
    Author's mail: dania.garcias@urv.cat josepa.gene@urv.cat
    Author identifier: 0000-0003-2397-1646 0000-0001-6195-9299
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/pii/S187861461600043X?via%3Dihub
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Fungal Biology. 120 (11): 1458-1467
    APA: Siqueira J; Sutton D; García D; Gené J; Thomson P; Wiederhold N; Guarro J (2016). Species diversity of Aspergillus section Versicolores in clinical samples and antifungal susceptibility. Fungal Biology, 120(11), 1458-1467. DOI: 10.1016/j.funbio.2016.02.006
    Article's DOI: 10.1016/j.funbio.2016.02.006
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    Publication Type: Journal Publications
  • Keywords:

    Ecology, Evolution, Behavior and Systematics,Genetics,Infectious Diseases,Mycology,Plant Science
    Taxonomy
    Sydowii
    Penicillium
    Onychomycosis
    Multilocus phylogeny
    Metabolites
    Infection
    Identification
    Endolichenic fungus
    Aspergilli
    Ascomycetes
    Antufungal activity
    Amphotericin-b
    Agents
    multilocus phylogeny
    aspergilli
    ascomycetes
    antufungal activity
    Zootecnia / recursos pesqueiros
    Química
    Plant science
    Mycology
    Medicina veterinaria
    Medicina ii
    Medicina i
    Interdisciplinar
    Infectious diseases
    Genetics
    Farmacia
    Engenharias iv
    Economia
    Ecology, evolution, behavior and systematics
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biodiversidade
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