Articles producció científica> Química Analítica i Química Orgànica

Artificial receptors for the electrochemical detection of bacterial flagellar filaments from Proteus mirabilis

  • Identification data

    Identifier: imarina:6388967
    Authors:
    Khan MAires Cardoso ASales MMerino STomás JRius FRiu J
    Abstract:
    © 2017 In this paper for the first time we successfully detect bacterial flagellar filaments from Proteus mirabilis using molecularly imprinted artificial receptors. These receptors acted as a sensing layer of the biosensors, assembled by imprinting flagellar proteins onto a polymeric backbone of electropolymerized phenol. In short, flagellar filaments were absorbed onto a carbon support, phenol was electropolymerized around it through the carbon conductive matrix to create the protein molecular molds, and finally the flagellar proteins were removed by enzymatic and electrochemical action. Each removed flagellar protein gave rise to an imprinted site with eventual rebinding ability. Electrical impedance spectroscopy (EIS) and square wave voltammetry (SWV) were employed to measure the interaction of flagellar filaments with the sensing layer assembled on commercial screen-printed electrodes, providing low detection limits, high precision and selectivity toward the targeted protein. The detection limit was 0.7 ng/mL by EIS and 0.9 ng/mL by SWV. The artificial receptors were further assembled on home-made paper-printed electrodes, with the three-electrode system printed on a paper substrate, offering the possibility of detecting flagellar filaments at as low as 0.6 ng/mL with a disposable and cost-effective portable device. To the best of our knowledge this is the first sensing device where molecularly imprinted artificial receptors are tailored on home-made electrode based on paper substrates with three electrodes assembled together, which is a suitable approach for the fabrication of easy and cost-effective tailored electrodes.
  • Others:

    Author, as appears in the article.: Khan M; Aires Cardoso A; Sales M; Merino S; Tomás J; Rius F; Riu J
    Department: Química Analítica i Química Orgànica
    URV's Author/s: Khan, Muhammad Faizan / Riu Rusell, Jordi / Rius Ferrús, Francisco Javier / RIUS RUIZ, FRANCESC XAVIER
    Keywords: Screen-printed electrodes Proteus mirabilis Paper-printed electrodes Molecularly imprinted polymers Flagellar filaments Electropolymerization Disposable device Artificial receptors
    Abstract: © 2017 In this paper for the first time we successfully detect bacterial flagellar filaments from Proteus mirabilis using molecularly imprinted artificial receptors. These receptors acted as a sensing layer of the biosensors, assembled by imprinting flagellar proteins onto a polymeric backbone of electropolymerized phenol. In short, flagellar filaments were absorbed onto a carbon support, phenol was electropolymerized around it through the carbon conductive matrix to create the protein molecular molds, and finally the flagellar proteins were removed by enzymatic and electrochemical action. Each removed flagellar protein gave rise to an imprinted site with eventual rebinding ability. Electrical impedance spectroscopy (EIS) and square wave voltammetry (SWV) were employed to measure the interaction of flagellar filaments with the sensing layer assembled on commercial screen-printed electrodes, providing low detection limits, high precision and selectivity toward the targeted protein. The detection limit was 0.7 ng/mL by EIS and 0.9 ng/mL by SWV. The artificial receptors were further assembled on home-made paper-printed electrodes, with the three-electrode system printed on a paper substrate, offering the possibility of detecting flagellar filaments at as low as 0.6 ng/mL with a disposable and cost-effective portable device. To the best of our knowledge this is the first sensing device where molecularly imprinted artificial receptors are tailored on home-made electrode based on paper substrates with three electrodes assembled together, which is a suitable approach for the fabrication of easy and cost-effective tailored electrodes.
    Thematic Areas: Surfaces, coatings and films Spectroscopy Química Odontología Nutrição Metals and alloys Medicina ii Medicina i Materials chemistry Materiais Interdisciplinar Instruments & instrumentation Instrumentation Farmacia Engenharias iv Engenharias iii Engenharias ii Engenharias i Electronic, optical and magnetic materials Electrochemistry Electrical and electronic engineering Educação física Educação Economia Condensed matter physics Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 09254005
    Author's mail: muhammadfaizan.khan@urv.cat jordi.riu@urv.cat
    Author identifier: 0000-0001-5823-9223
    Record's date: 2024-08-10
    Papper version: info:eu-repo/semantics/submittedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0925400517300187
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Sensors And Actuators B-Chemical. 244 732-741
    APA: Khan M; Aires Cardoso A; Sales M; Merino S; Tomás J; Rius F; Riu J (2017). Artificial receptors for the electrochemical detection of bacterial flagellar filaments from Proteus mirabilis. Sensors And Actuators B-Chemical, 244(), 732-741. DOI: 10.1016/j.snb.2017.01.018
    Article's DOI: 10.1016/j.snb.2017.01.018
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    Publication Type: Journal Publications
  • Keywords:

    Chemistry, Analytical,Condensed Matter Physics,Electrical and Electronic Engineering,Electrochemistry,Electronic, Optical and Magnetic Materials,Instrumentation,Instruments & Instrumentation,Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films
    Screen-printed electrodes
    Proteus mirabilis
    Paper-printed electrodes
    Molecularly imprinted polymers
    Flagellar filaments
    Electropolymerization
    Disposable device
    Artificial receptors
    Surfaces, coatings and films
    Spectroscopy
    Química
    Odontología
    Nutrição
    Metals and alloys
    Medicina ii
    Medicina i
    Materials chemistry
    Materiais
    Interdisciplinar
    Instruments & instrumentation
    Instrumentation
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Electronic, optical and magnetic materials
    Electrochemistry
    Electrical and electronic engineering
    Educação física
    Educação
    Economia
    Condensed matter physics
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Astronomia / física
    Analytical chemistry
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