Author, as appears in the article.: Patraca, Ivan; Martinez, Nohora; Busquets, Oriol; Marti, Aleix; Pedros, Ignacio; Beas-Zarate, Carlos; Marin, Miguel; Ettcheto, Miren; Sureda, Francesc; Auladell, Carme; Camins, Antoni; Folch, Jaume
Department: Ciències Mèdiques Bàsiques; Bioquímica i Biotecnologia
URV's Author/s: Folch Lopez, Jaume / Martí Zaragoza, Àlex / Martínez Micaelo, Nieves Beatriz / PATRACA FIERRO, JOSÉ IVÁN / Sureda Batlle, Francesc Xavier
Keywords: Tumor necrosis factor-alpha; P38 mitogen-activated protein kinases; P38 mapk; Nitric oxide; Neuroglia; Mice, inbred c57bl; Mice; Map kinase signaling system; Leptin; Interleukin-1beta; Interferon-gamma; Inos; Inflammation; Glial cells; Disease models, animal; Cytokines; Cytochrome-c; Cells, cultured; Cell survival; Caspase-3; Apoptosis; Anti-inflammatory agents; Animals; leptin; inos; glial cells; cytochrome-c; caspase-3
Abstract: © 2016 Institute of Pharmacology, Polish Academy of Sciences Background In the present work, we studied the modulatory effect of Leptin (Lep) against pro-inflammatory cytokines, tumour necrosis factor-alpha (TNFα), interleukin 1-beta (IL1β) and interferon-gamma (IFNγ), in primary glial cell cultures. Methods Glial cultures were treated with pro-inflammatory cytokines (TNFα, 20 ng/ml; IL1β, 20 ng/ml; IFNγ 20 ng/ml). Cells were pre-treated with Lep 500 nM, 1 h prior to cytokine treatment. NO released from glial cells was determined using the Griess reaction. Cell viability was determined by the MTT method. Protein expression was determined by western blot. Results Pre-treatment with 500 nM Lep produced an inhibitory effect on inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production after glial cells exposure to pro-inflammatory cytokines. Anti-inflammatory effect can be related to a decrease in P38 MAP Kinase (MAPK) pathway activity. Treatment of glial cell cultures with Lep also reduced the intrinsic apoptotic pathway (cytochrome c release and caspase-3 activation). Conclusions We suggest that Lep would act as an anti-inflammatory factor in glial cells exposed to pro-inflammatory cytokines, exerting its function on p38 MAPK pathway and reducing NO production.
Thematic Areas: Zootecnia / recursos pesqueiros; Saúde coletiva; Química; Psicología; Pharmacology & pharmacy; Pharmacology; Odontología; Nutrição; Medicine (miscellaneous); Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Interdisciplinar; Farmacia; Educação física; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Biotecnología
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 17341140
Author's mail: neus.martinez@urv.cat; alex.marti@estudiants.urv.cat; jaume.folch@urv.cat; francesc.sureda@urv.cat
Record's date: 2025-02-08
Paper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1734114016304273?via%3Dihub
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Pharmacological Reports. 69 (3): 409-418
APA: Patraca, Ivan; Martinez, Nohora; Busquets, Oriol; Marti, Aleix; Pedros, Ignacio; Beas-Zarate, Carlos; Marin, Miguel; Ettcheto, Miren; Sureda, Francesc (2017). Anti-inflammatory role of Leptin in glial cells through p38 MAPK pathway inhibition. Pharmacological Reports, 69(3), 409-418. DOI: 10.1016/j.pharep.2016.12.005
Article's DOI: 10.1016/j.pharep.2016.12.005
Entity: Universitat Rovira i Virgili
Journal publication year: 2017
Publication Type: Journal Publications
Repositori URV