Author, as appears in the article.: Patraca, Ivan; Martinez, Nohora; Busquets, Oriol; Marti, Aleix; Pedros, Ignacio; Beas-Zarate, Carlos; Marin, Miguel; Ettcheto, Miren; Sureda, Francesc; Auladell, Carme; Camins, Antoni; Folch, Jaume
Department: Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia
URV's Author/s: Folch Lopez, Jaume / Martí Zaragoza, Àlex / Martínez Micaelo, Nieves Beatriz / PATRACA FIERRO, JOSÉ IVÁN / Sureda Batlle, Francesc Xavier
Keywords: Tumor necrosis factor-alpha P38 mitogen-activated protein kinases P38 mapk Nitric oxide Neuroglia Mice, inbred c57bl Mice Map kinase signaling system Leptin Interleukin-1beta Interferon-gamma Inos Inflammation Glial cells Disease models, animal Cytokines Cytochrome-c Cells, cultured Cell survival Caspase-3 Apoptosis Anti-inflammatory agents Animals leptin inos glial cells cytochrome-c caspase-3
Abstract: © 2016 Institute of Pharmacology, Polish Academy of Sciences Background In the present work, we studied the modulatory effect of Leptin (Lep) against pro-inflammatory cytokines, tumour necrosis factor-alpha (TNFα), interleukin 1-beta (IL1β) and interferon-gamma (IFNγ), in primary glial cell cultures. Methods Glial cultures were treated with pro-inflammatory cytokines (TNFα, 20 ng/ml; IL1β, 20 ng/ml; IFNγ 20 ng/ml). Cells were pre-treated with Lep 500 nM, 1 h prior to cytokine treatment. NO released from glial cells was determined using the Griess reaction. Cell viability was determined by the MTT method. Protein expression was determined by western blot. Results Pre-treatment with 500 nM Lep produced an inhibitory effect on inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production after glial cells exposure to pro-inflammatory cytokines. Anti-inflammatory effect can be related to a decrease in P38 MAP Kinase (MAPK) pathway activity. Treatment of glial cell cultures with Lep also reduced the intrinsic apoptotic pathway (cytochrome c release and caspase-3 activation). Conclusions We suggest that Lep would act as an anti-inflammatory factor in glial cells exposed to pro-inflammatory cytokines, exerting its function on p38 MAPK pathway and reducing NO production.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Pharmacology & pharmacy Pharmacology Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Biotecnología
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 17341140
Author's mail: neus.martinez@urv.cat alex.marti@estudiants.urv.cat jaume.folch@urv.cat francesc.sureda@urv.cat
Author identifier: 0000-0002-5051-8858 0000-0002-7968-3929
Record's date: 2024-10-19
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1734114016304273?via%3Dihub
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Pharmacological Reports. 69 (3): 409-418
APA: Patraca, Ivan; Martinez, Nohora; Busquets, Oriol; Marti, Aleix; Pedros, Ignacio; Beas-Zarate, Carlos; Marin, Miguel; Ettcheto, Miren; Sureda, Francesc (2017). Anti-inflammatory role of Leptin in glial cells through p38 MAPK pathway inhibition. Pharmacological Reports, 69(3), 409-418. DOI: 10.1016/j.pharep.2016.12.005
Article's DOI: 10.1016/j.pharep.2016.12.005
Entity: Universitat Rovira i Virgili
Journal publication year: 2017
Publication Type: Journal Publications
Repositori URV