Author, as appears in the article.: Ráki M; Dahal-Koirala S; Yu H; Korponay-Szabó I; Gyimesi J; Castillejo G; Jahnsen J; Qiao S; Sollid L

Department: Medicina i Cirurgia

URV's Author/s: Castillejo De Villasante, Gemma

Keywords: Tg2 Preventcd study Cd4 t cell + Autoimmunity preventcd study cd4(+) t cell autoimmunity

Abstract: © 2017 AGA Institute Background & Aims Celiac disease is a chronic small intestinal inflammatory disorder mediated by an immune response to gluten peptides in genetically susceptible individuals. Celiac disease is often diagnosed in early childhood, but some patients receive a diagnosis late in life. It is uncertain whether pediatric celiac disease is distinct from adult celiac disease. It has been proposed that gluten-reactive T cells in children recognize deamidated and native gluten epitopes, whereas T cells from adults only recognize deamidated gluten peptides. We studied the repertoire of gluten epitopes recognized by T cells from children and adults. Methods We examined T-cell responses against gluten by generating T-cell lines and T-cell clones from intestinal biopsies of adults and children and tested proliferative response to various gluten peptides. We analyzed T cells from 14 children (2−5 years old) at high risk for celiac disease who were followed for celiac disease development. We also analyzed T cells from 6 adults (26−55 years old) with untreated celiac disease. All children and adults were positive for HLA-DQ2.5. Biopsies were incubated with gluten digested with chymotrypsin (modified or unmodified by the enzyme transglutaminase 2) or the peptic-tryptic digest of gliadin (in native and deamidated forms) before T-cell collection. Results Levels of T-cell responses were higher to deamidated gluten than to native gluten in children and adults. T cells from children and adults each reacted to multiple gluten epitopes. Several T-cell clones were cross-reactive, especially clones that recognized epitopes from γ-and ω-gliadin. About half of the generated T-cell clones from children and adults reacted to unknown epitopes. Conclusions T-cell responses to different gluten peptides appear to be similar between adults and children at the time of diagnosis of celiac disease.

Thematic Areas: Saúde coletiva Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Matemática / probabilidade e estatística Interdisciplinar Hepatology General medicine Gastroenterology & hepatology Gastroenterology Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 00165085

Author's mail: gemma.castillejo@urv.cat

Record's date: 2023-02-26

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://www.gastrojournal.org/article/S0016-5085(17)35625-1/fulltext?referrer=https%3A%2F%2Firis.urv.cat%2F#%20

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Gastroenterology. 153 (3): 787-798.e4

APA: Ráki M; Dahal-Koirala S; Yu H; Korponay-Szabó I; Gyimesi J; Castillejo G; Jahnsen J; Qiao S; Sollid L (2017). Similar Responses of Intestinal T Cells From Untreated Children and Adults With Celiac Disease to Deamidated Gluten Epitopes. Gastroenterology, 153(3), 787-798.e4. DOI: 10.1053/j.gastro.2017.05.016

Article's DOI: 10.1053/j.gastro.2017.05.016

Entity: Universitat Rovira i Virgili

Journal publication year: 2017

Publication Type: Journal Publications