Similar Responses of Intestinal T Cells From Untreated Children and Adults With Celiac Disease to Deamidated Gluten Epitopes

Identifier:  imarina:6389234

Handle: https://hdl.handle.net/20.500.11797/imarina6389234

Authors:  Ráki, M; Dahal-Koirala, S; Yu, H; Korponay-Szabó, IR; Gyimesi, J; Castillejo, G; Jahnsen, J; Qiao, SW; Sollid, LM

Abstract:
© 2017 AGA Institute Background & Aims Celiac disease is a chronic small intestinal inflammatory disorder mediated by an immune response to gluten peptides in genetically susceptible individuals. Celiac disease is often diagnosed in early childhood, but some patients receive a diagnosis late in life. It is uncertain whether pediatric celiac disease is distinct from adult celiac disease. It has been proposed that gluten-reactive T cells in children recognize deamidated and native gluten epitopes, whereas T cells from adults only recognize deamidated gluten peptides. We studied the repertoire of gluten epitopes recognized by T cells from children and adults. Methods We examined T-cell responses against gluten by generating T-cell lines and T-cell clones from intestinal biopsies of adults and children and tested proliferative response to various gluten peptides. We analyzed T cells from 14 children (2−5 years old) at high risk for celiac disease who were followed for celiac disease development. We also analyzed T cells from 6 adults (26−55 years old) with untreated celiac disease. All children and adults were positive for HLA-DQ2.5. Biopsies were incubated with gluten digested with chymotrypsin (modified or unmodified by the enzyme transglutaminase 2) or the peptic-tryptic digest of gliadin (in native and deamidated forms) before T-cell collection. Results Levels of T-cell responses were higher to deamidated gluten than to native gluten in children and adults. T cells from children and adults each reacted to multiple gluten epitopes. Several T-cell clones were cross-reactive, especially clones that recognized epitopes from γ-and ω-gliadin. About half of the generated T-cell clones from children and adults reacted to unknown epitopes. Conclusions T-cell responses to different gluten peptides appear to be similar between adults and children at the time of diagnosis of celiac disease.