Project code: CTQ2016-77128-R
Keywords: Whole blood detection Potentiometry Paper-based sensor Monitoring glucose Mixed potential Glucose Electrode Electrochemical biosensors potentiometry paper-based sensor mixed potential glucose
Record's date: 2023-02-19
Papper version: info:eu-repo/semantics/acceptedVersion
Papper original source: Biosensors & Bioelectronics. 163 (112302): 112302-
APA: Cánovas R; Blondeau P; Andrade FJ (2020). Modulating the mixed potential for developing biosensors: Direct potentiometric determination of glucose in whole, undiluted blood. Biosensors & Bioelectronics, 163(112302), 112302-. DOI: 10.1016/j.bios.2020.112302
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Acronym: HOMESENS
Publication Type: Journal Publications
Author, as appears in the article.: Cánovas R; Blondeau P; Andrade FJ
Department: Química Analítica i Química Orgànica
URV's Author/s: Blondeau, Pascal Jean Claude Leon
Abstract: © 2020 Elsevier B.V. The growing demand for tools to generate chemical information in decentralized settings is creating a vast range of opportunities for potentiometric sensors, since their combination of robustness, simplicity of operation and cost can hardly be rivalled by any other technique. In previous works, we have shown that the mixed potential of a Pt electrode can be controlled with analytical purposes using a coating of Nafion, thus providing a way to develop a potentiometric biosensor for glucose. Unfortunately, the linear range of this device did not match the relevant clinical range for glucose in blood. This work presents a novel strategy to control the mixed potential that allows the development of a potentiometric biosensor for the direct detection of glucose in whole, undiluted blood without any sample pretreatment. By changing the ionomer, the analytical response can be tuned, shifting the linear range while keeping the sensitivity. Aquivion, a polyelectrolyte from the same family as Nafion, is used to stabilize the mixed potential of a platinized paper-based electrode, to entrap the enzyme and to reduce the interference from negatively charged species. Factors affecting the generation of the signal and the principle of detection are discussed. Optimization of the biosensor composition was achieved with particular focus on the characterization of the linear range and sensitivity. The accurate measurement of blood sugar levels in a single drop of whole blood with excellent recovery is presented.
Thematic Areas: Química Nanoscience and nanotechnology Nanoscience & nanotechnology Medicine (miscellaneous) Medicina ii Medicina i Materiais Interdisciplinar Farmacia Engenharias iv Engenharias iii Engenharias ii Electrochemistry Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Chemistry, analytical Biotecnología Biotechnology & applied microbiology Biotechnology Biophysics Biomedical engineering Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0956-5663
Author's mail: pascal.blondeau@urv.cat
Author identifier: 0000-0003-1331-5055
Journal volume: 163
Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0956566320302979?via%3Dihub
Funding program: Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad (Agencia Estatal de Investigación - Ministerio de Economía, Industria y Competitividad)
Article's DOI: 10.1016/j.bios.2020.112302
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Funding program action: Proyecto de I+D