Author, as appears in the article.: Jimenez-Lamana, Javier; Godin, Simon; Aragones, Gerard; Blade, Cinta; Szpunar, Joanna; Lobinski, Ryszard
Department: Bioquímica i Biotecnologia
URV's Author/s: Aragonès Bargalló, Gerard / BLADÉ SEGARRA, MARIA CINTA
Keywords: Toxicity; Skin; Silver nanoparticles; Protein induction; Nickel nanoparticles; Molecular-mechanisms; Mass-spectrometry; Human keratinocytes; High resolution mass spectrometry; Cytotoxicity; Culture-medium; Chemical carcinogens; Cells; Bioaccumulation; nickel nanoparticles; human keratinocytes; high resolution mass spectrometry; cytotoxicity
Abstract: © 2020 by the authors. Although nickel allergy and carcinogenicity are well known, their molecular mechanisms are still uncertain, thus demanding studies at the molecular level. The nickel carcinogenicity is known to be dependent on the chemical form of nickel, since only certain nickel compounds can enter the cell. This study investigates, for the first time, the cytotoxicity, cellular uptake, and molecular targets of nickel nanoparticles (NiNPs) in human skin cells in comparison with other chemical forms of nickel. The dose-response curve that was obtained for NiNPs in the cytotoxicity assays showed a linear behavior typical of genotoxic carcinogens. The exposure of keratinocytes to NiNPs leads to the release of Ni2+ ions and its accumulation in the cytosol. A 6 kDa nickel-binding molecule was found to be synthesized by cells exposed to NiNPs at a dose corresponding to medium mortality. This molecule was identified to be tumor-related p63-regulated gene 1 protein.
Thematic Areas: Physics, applied; Nanoscience & nanotechnology; Materials science, multidisciplinary; Materials science (miscellaneous); Materials science (all); General materials science; General chemical engineering; Engenharias ii; Chemistry, multidisciplinary; Chemical engineering (miscellaneous); Chemical engineering (all)
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 2079-4991
Author's mail: gerard.aragones@urv.cat
Record's date: 2025-02-18
Paper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2079-4991/10/5/992
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Nanomaterials. 10 (5): 992-
APA: Jimenez-Lamana, Javier; Godin, Simon; Aragones, Gerard; Blade, Cinta; Szpunar, Joanna; Lobinski, Ryszard (2020). Nickel nanoparticles induce the synthesis of a tumor-related polypeptide in human epidermal keratinocytes. Nanomaterials, 10(5), 992-. DOI: 10.3390/nano10050992
Article's DOI: 10.3390/nano10050992
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications
Repositori URV