Author, as appears in the article.: Alonso R; Girbau C; Martinez-Malaxetxebarria I; Pérez-Cataluña A; Salas-Massó N; Romalde JL; Figueras MJ; Fernandez-Astorga A
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Figueras Salvat, María Josefa / PÉREZ CATALUÑA, ALBA
Keywords: Wastewater Virulence genes Taxonomy Products Minimal standards Indoxyl acetate Hydrolysis Helicobacter Genetic diversity Carrot Campylobacter Arcobacter-butzleri Arcobacter Aliarcobacter vitoriensis sp. nov
Abstract: © 2020 Elsevier GmbH Two isolates, one recovered from a carrot and another one from urban wastewater, were characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates clustered together, and were most closely related to Aliarcobacter lanthieri. Multilocus phylogenetic analysis (MLPA) using the concatenated sequences of five housekeeping genes (atpA, gyrA, gyrB, hsp60 and rpoB) suggested that these isolates formed a distinct phylogenetic lineage among the genera derived from the former genus Arcobacter. Whole-genome sequence, in silico DNA-DNA hybridization (isDDH) and the average nucleotide identity (ANI) value between the genome of strain F199T and those of related species confirmed that these isolates represent a novel species. These strains can be differentiated from its phylogenetically closest species A. lanthieri by its inability to growth on 1% glycine and by their enzyme activity of esterase lipase (C8) and acid phosphatase. Our results, by the application of a polyphasic analysis, confirmed that these two isolates represent a novel species of the genus Aliarcobacter, for which the name Aliarcobacter vitoriensis sp. nov. is proposed. The type strain is F199T (=CECT 9230T=LMG 30050T).
Thematic Areas: Zootecnia / recursos pesqueiros Microbiology Interdisciplinar Engenharias iii Engenharias ii Ecology, evolution, behavior and systematics Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Biotecnología Biotechnology & applied microbiology Biodiversidade Applied microbiology and biotechnology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: mariajose.figueras@urv.cat
Author identifier: 0000-0002-2268-8980
Record's date: 2023-02-26
Papper version: info:eu-repo/semantics/acceptedVersion
Papper original source: Systematic And Applied Microbiology. 43 (4):
APA: Alonso R; Girbau C; Martinez-Malaxetxebarria I; Pérez-Cataluña A; Salas-Massó N; Romalde JL; Figueras MJ; Fernandez-Astorga A (2020). Aliarcobacter vitoriensis sp. nov., isolated from carrot and urban wastewater. Systematic And Applied Microbiology, 43(4), -. DOI: 10.1016/j.syapm.2020.126091
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications
Repositori URV