Articles producció científica> Ciències Mèdiques Bàsiques

PCR Based Detection of Phase Variable Genes in Pakistani Based Clinical Helicobacter pylori Strains

  • Identification data

    Identifier: imarina:6478320
    Handle: https://hdl.handle.net/20.500.11797/imarina6478320
    Authors:
    Ahmad, HajraKhan, ImadKhan, Fida MuhammadRahman, Amjad UrMughal, ImranMurad, WaheedKhan, SalmanRahman, Faiz UrAhmad, FaisalAhmad, Sajjad
    Abstract:
    Background: The distribution pattern of phase-variable genes varies from strain to strain and from region to region. The present study was carried out to investigate the distribution pattern of phase-variable genes within Pakistan-based Helicobacter pylori strains and to analyze and compare them with strains prevalent in other parts of the world. Objectives: To determine the distribution pattern of phase-variable genes in H. pylori strains circulating in Pakistan. Patients and Methods: Biopsy samples were collected from 85 symptomatic patients suffering from various upper gastrointestinal tract symptoms. The biopsy specimens were chopped, then inoculated on H. pylori-specific media and incubated in a Campylobacter Gas Generating kit. Positive isolates were further confirmed via staining and biochemical procedures. Primers were designed for five phase-variable genes using OligoCalc, an oligonucleotide properties calculator (version 3.26) according to parameters stipulated in the literature. Polymerase chain reaction (PCR) was performed on all positive isolates to determine the presence or absence of phase-variable genes. Results: On culturing, the prevalence of H. pylori infections in the samples was 44.7%. The prevalence was higher in females than in males, and it increased with age. PCR amplification revealed that the hsdR gene was present in 79% of samples, while the mod and beta-subunit genes were present in 16% and 30% of samples, respectively. The streptococcal M protein gene was found in 79%, while the fliP gene was prevalent in 56%. Conclusions: The distribution patterns of phase-variable genes in Pakistani H. pylori strains were found to be somewhat different. The dominant prevalence of the hsdR gene was an interesting finding, considering its role in bacterial

  • Others:

    Author, as appears in the article.: Ahmad, Hajra Khan, Imad Khan, Fida Muhammad Rahman, Amjad Ur Mughal, Imran Murad, Waheed Khan, Salman Rahman, Faiz Ur Ahmad, Faisal Ahmad, Sajjad
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: UR, FAIZ
    Keywords: Sequence Restriction modification system Phase-variable genes Pcr Infection Helicobacter pylori Genome Diversity Adaptation phase-variable genes pcr helicobacter pylori
    Abstract: Background: The distribution pattern of phase-variable genes varies from strain to strain and from region to region. The present study was carried out to investigate the distribution pattern of phase-variable genes within Pakistan-based Helicobacter pylori strains and to analyze and compare them with strains prevalent in other parts of the world. Objectives: To determine the distribution pattern of phase-variable genes in H. pylori strains circulating in Pakistan. Patients and Methods: Biopsy samples were collected from 85 symptomatic patients suffering from various upper gastrointestinal tract symptoms. The biopsy specimens were chopped, then inoculated on H. pylori-specific media and incubated in a Campylobacter Gas Generating kit. Positive isolates were further confirmed via staining and biochemical procedures. Primers were designed for five phase-variable genes using OligoCalc, an oligonucleotide properties calculator (version 3.26) according to parameters stipulated in the literature. Polymerase chain reaction (PCR) was performed on all positive isolates to determine the presence or absence of phase-variable genes. Results: On culturing, the prevalence of H. pylori infections in the samples was 44.7%. The prevalence was higher in females than in males, and it increased with age. PCR amplification revealed that the hsdR gene was present in 79% of samples, while the mod and beta-subunit genes were present in 16% and 30% of samples, respectively. The streptococcal M protein gene was found in 79%, while the fliP gene was prevalent in 56%. Conclusions: The distribution patterns of phase-variable genes in Pakistani H. pylori strains were found to be somewhat different. The dominant prevalence of the hsdR gene was an interesting finding, considering its role in bacterial defense in both micro- and macroenvironments.
    Thematic Areas: Microbiology (medical) Microbiology Infectious diseases
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 20083645
    Author's mail: faiz.urrahman@irta.cat
    Author identifier: 0000-0001-6965-2355
    Record's date: 2021-04-17
    Papper version: info:eu-repo/semantics/publishedVersion
    Papper original source: Jundishapur Journal Of Microbiology. 9 (7): e31824-
    APA: Ahmad, Sajjad; Ahmad, Faisal; Rahman, Faiz Ur; Khan, Salman; Murad, Waheed; Mughal, Imran; Rahman, Amjad Ur; Khan, Fida Muhammad; Khan, Imad; Ahmad, H (2016). PCR Based Detection of Phase Variable Genes in Pakistani Based Clinical Helicobacter pylori Strains. Jundishapur Journal Of Microbiology, 9(7), e31824-. DOI: 10.5812/jjm.31824
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    Publication Type: Journal Publications

  • Keywords:

    Infectious Diseases,Microbiology,Microbiology (Medical)
    Sequence
    Restriction modification system
    Phase-variable genes
    Pcr
    Infection
    Helicobacter pylori
    Genome
    Diversity
    Adaptation
    phase-variable genes
    pcr
    helicobacter pylori
    Microbiology (medical)
    Microbiology
    Infectious diseases

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