Articles producció científica> Bioquímica i Biotecnologia

Mycobacterium tuberculosis and whole genome sequencing: a practical guide and online tools available for the clinical microbiologist

  • Identification data

    Identifier: imarina:6478326
    Authors:
    Satta, G.Atzeni, A.McHugh, T. D.
    Abstract:
    Whole genome sequencing (WGS) has the potential to revolutionize the diagnosis of Mycobacterium tuberculosis infection but the lack of bioinformatic expertise among clinical microbiologists is a barrier for adoption. Software products for analysis should be simple, free of charge, able to accept data directly from the sequencer (FASTQ files) and to provide the basic functionalities all-in-one. The main aim of this narrative review is to provide a practical guide for the clinical microbiologist, with little or no practical experience of WGS analysis, with a specific focus on software products tailor-made for M. tuberculosis analysis. With sequencing performed by an external provider, it is now feasible to implement WGS analysis in the routine clinical practice of any microbiology laboratory, with the potential to detect resistance weeks before traditional phenotypic culture methods, but the clinical microbiologist should be aware of the limitations of this approach. (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
  • Others:

    Author, as appears in the article.: Satta, G.; Atzeni, A.; McHugh, T. D.;
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Atzeni, Alessandro
    Keywords: Whole genome sequencing Rapid susceptibility testing Phylogenetic analysis Outbreak Online tools Mycobacterium tuberculosis Diagnosis Cultures Antibiotic-resistance rapid susceptibility testing phylogenetic analysis online tools mycobacterium tuberculosis
    Abstract: Whole genome sequencing (WGS) has the potential to revolutionize the diagnosis of Mycobacterium tuberculosis infection but the lack of bioinformatic expertise among clinical microbiologists is a barrier for adoption. Software products for analysis should be simple, free of charge, able to accept data directly from the sequencer (FASTQ files) and to provide the basic functionalities all-in-one. The main aim of this narrative review is to provide a practical guide for the clinical microbiologist, with little or no practical experience of WGS analysis, with a specific focus on software products tailor-made for M. tuberculosis analysis. With sequencing performed by an external provider, it is now feasible to implement WGS analysis in the routine clinical practice of any microbiology laboratory, with the potential to detect resistance weeks before traditional phenotypic culture methods, but the clinical microbiologist should be aware of the limitations of this approach. (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
    Thematic Areas: Saúde coletiva Química Odontología Microbiology (medical) Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Infectious diseases Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Biotecnología Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: alessandro.atzeni@urv.cat alessandro.atzeni@urv.cat
    ISSN: 1198743X
    Author identifier: 0000-0002-1804-8606 0000-0002-1804-8606
    Record's date: 2023-05-14
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(16)30392-5/fulltext
    Papper original source: Clinical Microbiology And Infection. 23 (2): 69-72
    APA: Satta, G.; Atzeni, A.; McHugh, T. D.; (2017). Mycobacterium tuberculosis and whole genome sequencing: a practical guide and online tools available for the clinical microbiologist. Clinical Microbiology And Infection, 23(2), 69-72. DOI: 10.1016/j.cmi.2016.09.005
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.cmi.2016.09.005
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    Publication Type: Journal Publications
  • Keywords:

    Infectious Diseases,Medicine (Miscellaneous),Microbiology,Microbiology (Medical)
    Whole genome sequencing
    Rapid susceptibility testing
    Phylogenetic analysis
    Outbreak
    Online tools
    Mycobacterium tuberculosis
    Diagnosis
    Cultures
    Antibiotic-resistance
    rapid susceptibility testing
    phylogenetic analysis
    online tools
    mycobacterium tuberculosis
    Saúde coletiva
    Química
    Odontología
    Microbiology (medical)
    Microbiology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Infectious diseases
    Farmacia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Biotecnología
    Astronomia / física
    1198743X
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