Author, as appears in the article.: Taltavull N; Miralles-Pérez B; Nogués MR; Ramos-Romero S; Méndez L; Medina I; Torres JL; Romeu M
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Miralles Pérez, Bernat / Nogués Llort, Maria Rosa / Romeu Ferran, Marta / Taltavull Anglada, Núria
Keywords: Steatosis Sphingomyelin synthase 2 Proanthocyanidins Polyunsaturated fatty-acids Polyphenols Oxidative stress Omega-3 pufa Omega-3 Metabolism Liver Glucose Diacylglycerol Ceramide Biological samples Antioxidants polyphenols omega-3 diacylglycerol ceramide antioxidants
Abstract: Diacylglycerols (DAG) and ceramides have been suggested as early predictors of insulin resistance. This study was aimed to examine the combined effects of fish oil (FO) and grape seed extract (GSE) on hepatic endogenous antioxidants, DAG and ceramides in diet-induced early stages of insulin resistance. Thirty-five rats were fed one of the following diets: (1) a standard diet (STD group), (2) a high-fat high-sucrose diet (HFHS group), (3) an HFHS diet enriched with FO (FO group), (4) an HFHS diet enriched with GSE (GSE group) or (5) an HFHS diet enriched with FO and GSE (FO + GSE group). In the liver, endogenous antioxidants were measured using spectrophotometric and fluorometric techniques, and non-targeted lipidomics was conducted for the assessment of DAG and ceramides. After 24 weeks, the FO + GSE group showed increased glutathione peroxidase activity, as well as monounsaturated fatty acid and polyunsaturated fatty acid-containing DAG, and long-chain fatty acid-containing ceramides abundances compared to the STD group. The FO and GSE combination induced similar activation of the antioxidant system and bioactive lipid accumulation in the liver than the HFHS diet without supplementation. In addition, the FO and GSE combination increased the abundances of polyunsaturated fatty acid-containing DAG in the liver.
Thematic Areas: Zootecnia / recursos pesqueiros Química Pharmacology, toxicology and pharmaceutics (miscellaneous) Pharmacology & pharmacy Pharmaceutical science Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Geociências Farmacia Engenharias iii Engenharias ii Educação física Drug discovery Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Biotecnología Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 16603397
Author's mail: nuria.taltavull@urv.cat bmirallesperez@gmail.com marta.romeu@urv.cat
Author identifier: 0000-0002-3340-2650 0000-0003-1294-7069 0000-0002-2131-1858
Record's date: 2024-10-26
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/1660-3397/18/6/318
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Marine Drugs. 18 (6):
APA: Taltavull N; Miralles-Pérez B; Nogués MR; Ramos-Romero S; Méndez L; Medina I; Torres JL; Romeu M (2020). Effects of Fish Oil and Grape Seed Extract Combination on Hepatic Endogenous Antioxidants and Bioactive Lipids in Diet-Induced Early Stages of Insulin Resistance in Rats. Marine Drugs, 18(6), -. DOI: 10.3390/md18060318
Article's DOI: 10.3390/md18060318
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications