Articles producció científica> Química Física i Inorgànica

Bifunctional Tm3+,Yb3+:GdVO4@SiO2 Core-Shell Nanoparticles in HeLa Cells: Upconversion Luminescence Nanothermometry in the First Biological Window and Biolabelling in the Visible

  • Identification data

    Identifier: imarina:6684415
    Authors:
    Savchuk, OleksandrCarvajal Marti, Joan JosepCascales, ConcepcionHaro-Gonzalez, PatriciaSanz-Rodriguez, FranciscoAguilo, MagdalenaDiaz, Francesc
    Abstract:
    The bifunctional possibilities of Tm,Yb:GdVO4@SiO2 core-shell nanoparticles for temperature sensing by using the near-infrared (NIR)-excited upconversion emissions in the first biological window, and biolabeling through the visible emissions they generate, were investigated. The two emission lines located at 700 and 800 nm, that arise from the thermally coupled F-3(2,3) and H-3(4) energy levels of Tm3+, were used to develop a luminescent thermometer, operating through the Fluorescence Intensity Ratio (FIR) technique, with a very high thermal relative sensitivity. Moreover, since the inert shell surrounding the luminescent active core allows for dispersal of the nanoparticles in water and biological compatible fluids, we investigated the penetration depth that can be realized in biological tissues with their emissions in the NIR range, achieving a value of 0.8 mm when excited at powers of 50 mW. After their internalization in HeLa cells, a low toxicity was observed and the potentiality for biolabelling in the visible range was demonstrated, which facilitated the identification of the location of the nanoparticles inside the cells, and the temperature determination.
  • Others:

    Author, as appears in the article.: Savchuk, Oleksandr; Carvajal Marti, Joan Josep; Cascales, Concepcion; Haro-Gonzalez, Patricia; Sanz-Rodriguez, Francisco; Aguilo, Magdalena; Diaz, Francesc;
    Department: Química Física i Inorgànica
    URV's Author/s: Aguiló Díaz, Magdalena / Carvajal Martí, Joan Josep / Díaz González, Francisco Manuel
    Keywords: Working Upconverting nanoparticles Tm3+ Thermometry Sensitivity Platform Luminescence thermometry Hela cells Core-shell nanoparticles Biological windows Biolabeling hela cells core-shell nanoparticles biological windows biolabeling
    Abstract: The bifunctional possibilities of Tm,Yb:GdVO4@SiO2 core-shell nanoparticles for temperature sensing by using the near-infrared (NIR)-excited upconversion emissions in the first biological window, and biolabeling through the visible emissions they generate, were investigated. The two emission lines located at 700 and 800 nm, that arise from the thermally coupled F-3(2,3) and H-3(4) energy levels of Tm3+, were used to develop a luminescent thermometer, operating through the Fluorescence Intensity Ratio (FIR) technique, with a very high thermal relative sensitivity. Moreover, since the inert shell surrounding the luminescent active core allows for dispersal of the nanoparticles in water and biological compatible fluids, we investigated the penetration depth that can be realized in biological tissues with their emissions in the NIR range, achieving a value of 0.8 mm when excited at powers of 50 mW. After their internalization in HeLa cells, a low toxicity was observed and the potentiality for biolabelling in the visible range was demonstrated, which facilitated the identification of the location of the nanoparticles inside the cells, and the temperature determination.
    Thematic Areas: Physics, applied Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General chemical engineering Engenharias ii Chemistry, multidisciplinary Chemical engineering (miscellaneous) Chemical engineering (all)
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 20794991
    Author's mail: f.diaz@urv.cat magdalena.aguilo@urv.cat joanjosep.carvajal@urv.cat
    Author identifier: 0000-0003-4581-4967 0000-0001-6130-9579 0000-0002-4389-7298
    Record's date: 2024-04-27
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2079-4991/10/5/993
    Papper original source: Nanomaterials. 10 (5):
    APA: Savchuk, Oleksandr; Carvajal Marti, Joan Josep; Cascales, Concepcion; Haro-Gonzalez, Patricia; Sanz-Rodriguez, Francisco; Aguilo, Magdalena; Diaz, Fra (2020). Bifunctional Tm3+,Yb3+:GdVO4@SiO2 Core-Shell Nanoparticles in HeLa Cells: Upconversion Luminescence Nanothermometry in the First Biological Window and Biolabelling in the Visible. Nanomaterials, 10(5), -. DOI: 10.3390/nano10050993
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.3390/nano10050993
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2020
    Publication Type: Journal Publications
  • Keywords:

    Chemical Engineering (Miscellaneous),Chemistry, Multidisciplinary,Materials Science (Miscellaneous),Materials Science, Multidisciplinary,Nanoscience & Nanotechnology,Physics, Applied
    Working
    Upconverting nanoparticles
    Tm3+
    Thermometry
    Sensitivity
    Platform
    Luminescence thermometry
    Hela cells
    Core-shell nanoparticles
    Biological windows
    Biolabeling
    hela cells
    core-shell nanoparticles
    biological windows
    biolabeling
    Physics, applied
    Nanoscience & nanotechnology
    Materials science, multidisciplinary
    Materials science (miscellaneous)
    Materials science (all)
    General materials science
    General chemical engineering
    Engenharias ii
    Chemistry, multidisciplinary
    Chemical engineering (miscellaneous)
    Chemical engineering (all)
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