Author, as appears in the article.: Savchuk, Oleksandr; Carvajal Marti, Joan Josep; Cascales, Concepcion; Haro-Gonzalez, Patricia; Sanz-Rodriguez, Francisco; Aguilo, Magdalena; Diaz, Francesc;

Department: Química Física i Inorgànica

URV's Author/s: Aguiló Díaz, Magdalena / Carvajal Martí, Joan Josep / Díaz González, Francisco Manuel

Keywords: Working Upconverting nanoparticles Tm3+ Thermometry Sensitivity Platform Luminescence thermometry Hela cells Core-shell nanoparticles Biological windows Biolabeling hela cells core-shell nanoparticles biological windows biolabeling

Abstract: The bifunctional possibilities of Tm,Yb:GdVO4@SiO2 core-shell nanoparticles for temperature sensing by using the near-infrared (NIR)-excited upconversion emissions in the first biological window, and biolabeling through the visible emissions they generate, were investigated. The two emission lines located at 700 and 800 nm, that arise from the thermally coupled F-3(2,3) and H-3(4) energy levels of Tm3+, were used to develop a luminescent thermometer, operating through the Fluorescence Intensity Ratio (FIR) technique, with a very high thermal relative sensitivity. Moreover, since the inert shell surrounding the luminescent active core allows for dispersal of the nanoparticles in water and biological compatible fluids, we investigated the penetration depth that can be realized in biological tissues with their emissions in the NIR range, achieving a value of 0.8 mm when excited at powers of 50 mW. After their internalization in HeLa cells, a low toxicity was observed and the potentiality for biolabelling in the visible range was demonstrated, which facilitated the identification of the location of the nanoparticles inside the cells, and the temperature determination.

Thematic Areas: Physics, applied Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General chemical engineering Engenharias ii Chemistry, multidisciplinary Chemical engineering (miscellaneous) Chemical engineering (all)

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 20794991

Author's mail: f.diaz@urv.cat magdalena.aguilo@urv.cat joanjosep.carvajal@urv.cat

Author identifier: 0000-0003-4581-4967 0000-0001-6130-9579 0000-0002-4389-7298

Record's date: 2023-09-23

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2079-4991/10/5/993

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Nanomaterials. 10 (5):

APA: Savchuk, Oleksandr; Carvajal Marti, Joan Josep; Cascales, Concepcion; Haro-Gonzalez, Patricia; Sanz-Rodriguez, Francisco; Aguilo, Magdalena; Diaz, Fra (2020). Bifunctional Tm3+,Yb3+:GdVO4@SiO2 Core-Shell Nanoparticles in HeLa Cells: Upconversion Luminescence Nanothermometry in the First Biological Window and Biolabelling in the Visible. Nanomaterials, 10(5), -. DOI: 10.3390/nano10050993

Article's DOI: 10.3390/nano10050993

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications