Articles producció científica> Bioquímica i Biotecnologia

Genetic and transcriptomic evidences suggest ARO10 genes are involved in benzenoid biosynthesis by yeast

  • Identification data

    Identifier: imarina:7067809
    Authors:
    Jose Valera, MariaZeida, AriBoido, EduardoBeltran, GemmaJesus Torija, MariaMas, AlbertRadi, RafaelDellacassa, EduardoCarrau, Francisco
    Abstract:
    © 2020 John Wiley & Sons, Ltd. Benzenoids are compounds associated with floral and fruity flavours in flowers, fruits and leaves and present a role in hormonal signalling in plants. These molecules are produced by the phenyl ammonia lyase pathway. However, some yeasts can also synthesize them from aromatic amino acids using an alternative pathway that remains unknown. Hanseniaspora vineae can produce benzenoids at levels up to two orders of magnitude higher than Saccharomyces species, so it is a model microorganism for studying benzenoid biosynthesis pathways in yeast. According to their genomes, several enzymes have been proposed to be involved in a mandelate pathway similar to that described for some prokaryotic cells. Among them, the ARO10 gene product could present benzoylformate decarboxylase activity. This enzyme catalyses the decarboxylation of benzoylformate into benzaldehyde at the end of the mandelate pathway in benzyl alcohol formation. Two homologous genes of ARO10 were found in the two sequenced H. vineae strains. In this study, nine other H. vineae strains were analysed to detect the presence and per cent homology of ARO10 sequences by PCR using specific primers designed for this species. Also, the copy number of the genes was estimated by quantitative PCR. To verify the relation of ARO10 with the production of benzyl alcohol during fermentation, a deletion mutant in the ARO10 gene of Saccharomyces cerevisiae was used. The two HvARO10 paralogues were analysed and compared with other α-ketoacid decarboxylases at the sequence and structural level.
  • Others:

    Author, as appears in the article.: Jose Valera, Maria; Zeida, Ari; Boido, Eduardo; Beltran, Gemma; Jesus Torija, Maria; Mas, Albert; Radi, Rafael; Dellacassa, Eduardo; Carrau, Francisco
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Beltran Casellas, Gemma / Mas Baron, Alberto / Torija Martínez, María Jesús / VALERA MARTÍNEZ, MARÍA JOSÉ
    Keywords: Wine yeast Transcriptome Substrate-specificity Saccharomyces cerevisiae proteins Saccharomyces cerevisiae Pyruvate decarboxylase Phenylpyruvate decarboxylase Pathway L-phenylalanine Hanseniaspora Fermentation Diversity Crystal-structure Coenzyme q Biosynthetic pathways Benzyl alcohol Benzoylformate decarboxylase Benzene derivatives Benzaldehydes Benzaldehyde Assimilable nitrogen Aroma compounds Aro10 protein, s cerevisiae Angstrom resolution Acid 4-hydroxybenzaldehyde coenzyme q benzoylformate decarboxylase 4-hydroxybenzaldehyde
    Abstract: © 2020 John Wiley & Sons, Ltd. Benzenoids are compounds associated with floral and fruity flavours in flowers, fruits and leaves and present a role in hormonal signalling in plants. These molecules are produced by the phenyl ammonia lyase pathway. However, some yeasts can also synthesize them from aromatic amino acids using an alternative pathway that remains unknown. Hanseniaspora vineae can produce benzenoids at levels up to two orders of magnitude higher than Saccharomyces species, so it is a model microorganism for studying benzenoid biosynthesis pathways in yeast. According to their genomes, several enzymes have been proposed to be involved in a mandelate pathway similar to that described for some prokaryotic cells. Among them, the ARO10 gene product could present benzoylformate decarboxylase activity. This enzyme catalyses the decarboxylation of benzoylformate into benzaldehyde at the end of the mandelate pathway in benzyl alcohol formation. Two homologous genes of ARO10 were found in the two sequenced H. vineae strains. In this study, nine other H. vineae strains were analysed to detect the presence and per cent homology of ARO10 sequences by PCR using specific primers designed for this species. Also, the copy number of the genes was estimated by quantitative PCR. To verify the relation of ARO10 with the production of benzyl alcohol during fermentation, a deletion mutant in the ARO10 gene of Saccharomyces cerevisiae was used. The two HvARO10 paralogues were analysed and compared with other α-ketoacid decarboxylases at the sequence and structural level.
    Thematic Areas: Química Odontología Mycology Microbiology Medicine (miscellaneous) Medicina ii Medicina i Genetics General medicine Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology & applied microbiology Biotechnology Bioengineering Biodiversidade Biochemistry & molecular biology Biochemistry Applied microbiology and biotechnology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0749-503X
    Author's mail: gemma.beltran@urv.cat mjesus.torija@urv.cat albert.mas@urv.cat
    Author identifier: 0000-0002-7071-205X 0000-0001-6419-0745 0000-0002-0763-1679
    Record's date: 2024-10-12
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://onlinelibrary.wiley.com/doi/10.1002/yea.3508
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Yeast. 37 (9-10): 427-435
    APA: Jose Valera, Maria; Zeida, Ari; Boido, Eduardo; Beltran, Gemma; Jesus Torija, Maria; Mas, Albert; Radi, Rafael; Dellacassa, Eduardo; Carrau, Francisco (2020). Genetic and transcriptomic evidences suggest ARO10 genes are involved in benzenoid biosynthesis by yeast. Yeast, 37(9-10), 427-435. DOI: 10.1002/yea.3508
    Article's DOI: 10.1002/yea.3508
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2020
    Publication Type: Journal Publications
  • Keywords:

    Applied Microbiology and Biotechnology,Biochemistry,Biochemistry & Molecular Biology,Bioengineering,Biotechnology,Biotechnology & Applied Microbiology,Genetics,Medicine (Miscellaneous),Microbiology,Mycology
    Wine yeast
    Transcriptome
    Substrate-specificity
    Saccharomyces cerevisiae proteins
    Saccharomyces cerevisiae
    Pyruvate decarboxylase
    Phenylpyruvate decarboxylase
    Pathway
    L-phenylalanine
    Hanseniaspora
    Fermentation
    Diversity
    Crystal-structure
    Coenzyme q
    Biosynthetic pathways
    Benzyl alcohol
    Benzoylformate decarboxylase
    Benzene derivatives
    Benzaldehydes
    Benzaldehyde
    Assimilable nitrogen
    Aroma compounds
    Aro10 protein, s cerevisiae
    Angstrom resolution
    Acid
    4-hydroxybenzaldehyde
    coenzyme q
    benzoylformate decarboxylase
    4-hydroxybenzaldehyde
    Química
    Odontología
    Mycology
    Microbiology
    Medicine (miscellaneous)
    Medicina ii
    Medicina i
    Genetics
    General medicine
    Farmacia
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biotechnology & applied microbiology
    Biotechnology
    Bioengineering
    Biodiversidade
    Biochemistry & molecular biology
    Biochemistry
    Applied microbiology and biotechnology
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