Author, as appears in the article.: Lopez, Carlos; Bosch-Princep, Ramon; Orero, Guifre; Fontoura Balaguero, Laia; Korzynska, Anna; Garcia-Rojo, Marcial; Bueno, Gloria; del Milagro Fernandez-Carrobles, Maria; Roszkowiak, Lukasz; Callau Casanova, Cristina; Teresa Salvado-Usach, M; Jaen Martinez, Joaquin; Gibert-Ramos, Albert; Roso-Llorach, Albert; Gras Navarro, Andrea; Berenguer-Poblet, Marta; Llobera, Montse; Gil Garcia, Julia; Tomas, Barbara; Gesti, Vanessa; Laine, Eeva; Plancoulaine, Benoit; Baucells, Jordi; Lejeune, Marylene
Department: Infermeria
URV's Author/s: Berenguer Poblet, Marta / Gras Navarro, Andrea / Lejeune, Marylène Marie / Lopez Pablo, Carlos
Keywords: Tissue Survival Prognostic role Predictors Peritumoral Metastasis Macrophages Lymphocytes Immunotherapy Immune response Expression Dendritic cells Carcinoma Breast cancer Axillary lymph nodes
Abstract: Background. The axillary lymph nodes (ALNs) in breast cancer patients are the body regions to where tumoral cells most often first disseminate. The tumour immune response is important for breast cancer patient outcome, and some studies have evaluated its involvement in ALN metastasis development. Most studies have focused on the intratumoral immune response, but very few have evaluated the peritumoral immune response. The aim of the present article is to evaluate the immune infiltrates of the peritumoral area and their association with the presence of ALN metastases.
Methods. The concentration of 11 immune markers in the peritumoral areas was studied in 149 patients diagnosed with invasive breast carcinoma of no special type (half of whom had ALN metastasis at diagnosis) using tissue microarrays, immunohistochemistry and digital image analysis procedures. The differences in the concentration of the immune response of peritumoral areas between patients diagnosed with and without metastasis in their ALNs were evaluated. A multivariate logistic regression model was developed to identify the clinical-pathological variables and the peritumoral immune markers independently associated with having or not having ALN metastases at diagnosis.
Results. No statistically significant differences were found in the concentrations of the 11 immune markers between patients diagnosed with or without ALN metastases. Patients with metastases in their ALNs had a higher histological grade, more lymphovascular and perineural invasion and larger-diameter tumours. The multivariate analysis, after validation by bootstrap simulation, revealed that only tumour diameter (OR = 1.04; 95% CI [1.00-1.07]; p = 0.026), lymphovascular invasion (OR = 25.42; 95% CI [9.57-67.55]; p<0.001) and histological grades 2 (OR = 3.84; 95% CI [1.11-13.28]; p = 0.033) and 3 (OR = 5.18; 95% CI [1.40-19.17]; p = 0.014) were associated with the presence of ALN metastases at diagnosis. This study is one of the first to study the association of the peritumoral immune response with ALN metastasis. We did not find any association of peritumoral immune infiltrates with the presence of ALN metastasis. Nevertheless, this does not rule out the possibility that other peritumoral immune populations are associated with ALN metastasis. This matter needs to be examined in greater depth, broadening the types of peritumoral immune cells studied, and including new peritumoral areas, such as the germinal centres of the peritumoral tertiary lymphoid structures found in extensively infiltrated neoplastic lesions.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Neuroscience (miscellaneous) Neuroscience (all) Multidisciplinary sciences Medicine (miscellaneous) Medicina ii Medicina i Materiais Interdisciplinar Geociências General neuroscience General medicine General biochemistry,genetics and molecular biology General agricultural and biological sciences Ensino Engenharias iv Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência política e relações internacionais Ciência de alimentos Biotecnología Biodiversidade Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Astronomia / física Agricultural and biological sciences (miscellaneous) Agricultural and biological sciences (all) Administração pública e de empresas, ciências contábeis e turismo
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: carlos.lopez@urv.cat marylenemarie.lejeune@urv.cat marta.berenguer@urv.cat
Author identifier: 0000-0003-1248-3065 0000-0001-8441-9404 0000-0002-6631-3777
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://peerj.com/articles/9779/#
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Peerj. 8 (e9779): e9779-
APA: Lopez, Carlos; Bosch-Princep, Ramon; Orero, Guifre; Fontoura Balaguero, Laia; Korzynska, Anna; Garcia-Rojo, Marcial; Bueno, Gloria; del Milagro Fernan (2020). Peritumoral immune infiltrates in primary tumours are not associated with the presence of axillary lymph node metastasis in breast cancer: a retrospective cohort study. Peerj, 8(e9779), e9779-. DOI: 10.7717/peerj.9779
Article's DOI: 10.7717/peerj.9779
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications
Repositori URV