Repositori URV

Author, as appears in the article.: Kioroglou, Dimitrios; Mas, Albert; Portillo, Maria C

Department: Bioquímica i Biotecnologia

URV's Author/s: Kioroglou, Dimitrios / Mas Baron, Alberto / Portillo Guisado, Maria del Carmen

Keywords: Yeast Vbnc state Sulfur-dioxide Spoilage Pcr Oak barrels Identification High-throughput sequencing Fungal communities Enumeration Dekkera-bruxellensis Brettanomyces-bruxellensis Bacterial communities Aged wine Acid bacteria

Abstract: Wine aged in barrels or bottles is susceptible to alteration by microorganisms that affect the final product quality. However, our knowledge of the microbiota during aging and the factors modulating the microbial communities is still quite limited. The present work uses high-throughput sequencing (HTS) techniques to deal with the meta-taxonomic characterization of microbial consortia present in red wines along 12 months aging. The wines obtained from two different grape varieties were aged at two different cellars and compared based on time of wine aging in the barrels, previous usage of the barrels, and differences between wine aging in oak barrels or glass bottles. The aging in barrels did not significantly affect the microbial diversity but changed the structure and composition of fungal and bacterial populations. The main microorganisms driving these changes were the bacterial genera <i>Acetobacter</i>, <i>Oenococcus</i>, <i>Lactobacillus</i>, <i>Gluconobacter</i>, <i>Lactococcus</i>, and <i>Komagataeibacter</i> and the fungal genera <i>Malassezia</i>, <i>Hanseniaspora</i>, and <i>Torulaspora</i>. Our results showed that the oak barrels increased effect on the microbial diversity in comparison with the glass bottles, in which the microbial community was very similar to that of the wine introduced in the barrels at the beginning of the aging. Furthermore, wine in the bottles harbored higher proportion of <i>Lactobacillus</i> but lower proportion of <i>Acetobacter</i>. Finally, it seems that 1 year of previous usage of the barrels was not enough to induce significant changes in the diversity or composition of microbiota through aging compared with new barrels. This is the first meta-taxonomic study on microbial communities during wine aging and shows that the microorganism composition of barrel-aged wines was similar at both cellars. These results hint at the possibility of a common and stable microbiota after aging in the absence of exogenous alterations. Further corroborations on the current outcome would be valuable for the comparison and detection of microbial alterations during aging that could potentially prevent economic losses in the wine industry.

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Microbiology (medical) Microbiology Medicina veterinaria Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Geografía Geociências Farmacia Ensino Engenharias iii Engenharias ii Engenharias i Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1664302X

Author's mail: albert.mas@urv.cat carmen.portillo@urv.cat

Author identifier: 0000-0002-0763-1679 0000-0002-4576-0244

Record's date: 2024-06-28

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.frontiersin.org/articles/10.3389/fmicb.2020.562560/full

Papper original source: Frontiers In Microbiology. 11 (562560): 562560-

APA: Kioroglou, Dimitrios; Mas, Albert; Portillo, Maria C (2020). High-Throughput Sequencing Approach to Analyze the Effect of Aging Time and Barrel Usage on the Microbial Community Composition of Red Wines. Frontiers In Microbiology, 11(562560), 562560-. DOI: 10.3389/fmicb.2020.562560

Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/

Article's DOI: 10.3389/fmicb.2020.562560

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications