Author, as appears in the article.: Sunyer-Figueres, Merce; Vazquez, Jennifer; Mas, Albert; Torija, Maria-Jesus; Beltran, Gemma
Department: Bioquímica i Biotecnologia
URV's Author/s: Beltran Casellas, Gemma / Mas Baron, Alberto / Sunyer Figueres, Mercè / Torija Martínez, María Jesús
Keywords: Yeast-cells Yeast Time-course Prooxidant Oxidative stress Mitochondria Membrane-lipid composition Melatonin L-tryptophan metabolites Hypoxia Hydrogen peroxide Growth Gene-expression Cytochrome-c-oxidase Bioactive compound Antioxidant Alcoholic fermentation oxidative stress mitochondria melatonin hypoxia hydrogen peroxide bioactive compound antioxidant
Abstract: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Melatonin is a ubiquitous indolamine that plays important roles in various aspects of biological processes in mammals. In Saccharomyces cerevisiae, melatonin has been reported to exhibit antioxidant properties and to modulate the expression of some genes involved in endogenous defense systems. The aim of this study was to elucidate the role of supplemented melatonin at the transcriptional level in S. cerevisiae in the presence and absence of oxidative stress. This was achieved by exposing yeast cells pretreated with different melatonin concentrations to hydrogen peroxide and assessing the entry of melatonin into the cell and the yeast response at the transcriptional level (by microarray and qPCR analyses) and the physiological level (by analyzing changes in the lipid composition and mitochondrial activity). We found that exogenous melatonin crossed cellular membranes at nanomolar concentrations and modulated the expression of many genes, mainly downregulating the expression of mitochondrial genes in the absence of oxidative stress, triggering a hypoxia-like response, and upregulating them under stress, mainly the cytochrome complex and electron transport chain. Other categories that were enriched by the effect of melatonin were related to transport, antioxidant activity, signaling, and carbohydrate and lipid metabolism. The overall results suggest that melatonin is able to reprogram the cellular machinery to achieve tolerance to oxidative stress.
Thematic Areas: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 20763921
Author's mail: gemma.beltran@urv.cat merce.sunyer@estudiants.urv.cat merce.sunyer@estudiants.urv.cat mjesus.torija@urv.cat albert.mas@urv.cat
Author identifier: 0000-0002-7071-205X 0000-0001-6419-0745 0000-0002-0763-1679
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2076-3921/9/10/947
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Antioxidants. 9 (10): 1-26
APA: Sunyer-Figueres, Merce; Vazquez, Jennifer; Mas, Albert; Torija, Maria-Jesus; Beltran, Gemma (2020). Transcriptomic insights into the effect of melatonin in saccharomyces cerevisiae in the presence and absence of oxidative stress. Antioxidants, 9(10), 1-26. DOI: 10.3390/antiox9100947
Article's DOI: 10.3390/antiox9100947
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications
Repositori URV