Author, as appears in the article.: Parada D D; Peña KB; Gumá J; Guilarte C; Riu F

Department: Medicina i Cirurgia

URV's Author/s: Gumà Padró, José

Keywords: Viral transport medium Swab Sars‐ Sars-cov-2 Rt‐ Rt-pcr Receptor Pcr Nasopharynx Liquid‐ Liquid-based Immunohistochemical Cytology Cov‐ Covid‐ Covid-19 Based 2 19

Abstract: Introduction We describe cytologic and immunohistologic findings in virus transport medium on cases under investigation of SARS-CoV-2 infection. Methods Cytologic findings in cases under investigation of SARS-CoV-2 infection from one hundred consecutive nasopharyngeal swab were reviewed. Immunohistochemistry and SARSCoV-2 RT-PCR determination were performed to detect virus. Results No viral inclusions were noted in squamous cells obtained from virus transport medium. Immunohistochemical study with monoclonal antibody against SARS-CoV-2 viral nucleoprotein showed positivity in squamous cells. No positivity was present in others cellular components. Conclusions SARS-CoV-2 predominantly localizes squamous cells in cytology samples of patients with RT-PCR positive determination of SARSCoV-2. The results of the current study support the notion that the nasopharyngeal region is the anatomical station that SARS-CoV-2 infects first, and the infection can lead to the migration of the virus into the lower airways.

Thematic Areas: Pathology and forensic medicine Pathology Odontología Medicine (miscellaneous) Medicina iii Medicina ii Medicina i Interdisciplinar Histology General medicine Engenharias iv Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biotecnología Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: jose.guma@urv.cat

Author identifier: 0000-0001-7541-9832

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://onlinelibrary.wiley.com/doi/10.1111/his.14257

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Histopathology. 78 (4): 586-592

APA: Parada D D; Peña KB; Gumá J; Guilarte C; Riu F (2021). Liquid-based cytological and immunohistochemical study of nasopharyngeal swab from persons under investigation for SARS-CoV-2 infection. Histopathology, 78(4), 586-592. DOI: 10.1111/his.14257

Article's DOI: 10.1111/his.14257

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications