Author, as appears in the article.: Parada D D; Peña KB; Gumá J; Guilarte C; Riu F
Department: Medicina i Cirurgia
URV's Author/s: Gumà Padró, José / Parada Dominguez, David
Keywords: Viral transport medium Swab Sars‐ Sars-cov-2 Rt‐ Rt-pcr Receptor Pcr Nasopharynx Liquid‐ Liquid-based Immunohistochemical Cytology Cov‐ Covid‐ Covid-19 Based 2 19
Abstract: Introduction We describe cytologic and immunohistologic findings in virus transport medium on cases under investigation of SARS-CoV-2 infection.
Methods Cytologic findings in cases under investigation of SARS-CoV-2 infection from one hundred consecutive nasopharyngeal swab were reviewed. Immunohistochemistry and SARSCoV-2 RT-PCR determination were performed to detect virus.
Results No viral inclusions were noted in squamous cells obtained from virus transport medium. Immunohistochemical study with monoclonal antibody against SARS-CoV-2 viral nucleoprotein showed positivity in squamous cells. No positivity was present in others cellular components.
Conclusions SARS-CoV-2 predominantly localizes squamous cells in cytology samples of patients with RT-PCR positive determination of SARSCoV-2. The results of the current study support the notion that the nasopharyngeal region is the anatomical station that SARS-CoV-2 infects first, and the infection can lead to the migration of the virus into the lower airways.
Thematic Areas: Pathology and forensic medicine Pathology Odontología Medicine (miscellaneous) Medicina iii Medicina ii Medicina i Interdisciplinar Histology General medicine Engenharias iv Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biotecnología Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: david.parada@urv.cat jose.guma@urv.cat
Author identifier: 0000-0001-7541-9832
Record's date: 2024-07-27
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://onlinelibrary.wiley.com/doi/10.1111/his.14257
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Histopathology. 78 (4): 586-592
APA: Parada D D; Peña KB; Gumá J; Guilarte C; Riu F (2021). Liquid-based cytological and immunohistochemical study of nasopharyngeal swab from persons under investigation for SARS-CoV-2 infection. Histopathology, 78(4), 586-592. DOI: 10.1111/his.14257
Article's DOI: 10.1111/his.14257
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV