Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Magnetic nanoparticle decorated anodic alumina nanotubes for fluorescent detection of cathepsin B

  • Identification data

    Identifier: imarina:9002284
    Authors:
    Domagalski, J TXifre-Perez, ETabrizi, M AFerre-Borrull, JMarsal, L F
    Abstract:
    © 2020 Elsevier Inc. In this work, we present the process to provide anodic alumina nanotubes with magnetic responsivity based on magnetic nanoparticles. We demonstrate the possibility to cause the motion of these composite nanotubes under magnetic field, providing them with guided mobility. The obtained magnetic anodic alumina nanotubes are completely characterized and their potential to undergo selective and effective functionalization, and stimuli-responsive load release is demonstrated. For this purpose, protease-triggered release of fluorescent molecules loaded inside the magnetic anodic alumina nanotubes (MAANTs) by selective functionalization is performed. The inner walls of the MAANTs were selectively covered with protein padding of albumin-fluorescein isothiocyanate conjugate (FITC-BSA) through means of silanization. Protein functionalization was designed to undergo proteolytic hydrolysis in presence of cathepsin B- protease highly expressed during growth and initial stages of tumor metastasis – in order to cleave peptide bond of albumin and release fluorescent fragments of the protein. Proteolytic reaction with the enzyme is performed under acidic conditions. Presented arrangement is an exemplary combination of functionalities – which are vast – and of value for applications like drug delivery and biosensing applications.
  • Others:

    Author, as appears in the article.: Domagalski, J T; Xifre-Perez, E; Tabrizi, M A; Ferre-Borrull, J; Marsal, L F
    Department: Enginyeria Electrònica, Elèctrica i Automàtica
    URV's Author/s: DOMAGALSKI, JAKUB / Ferré Borrull, José / Marsal Garví, Luis Francisco / Xifré Pérez, Elisabet
    Project code: Grant agreement No. 713679
    Keywords: Stimuli-responsive release Self-assembly Magnetic properties Cathepsin b Anodic alumina nanotubes
    Abstract: © 2020 Elsevier Inc. In this work, we present the process to provide anodic alumina nanotubes with magnetic responsivity based on magnetic nanoparticles. We demonstrate the possibility to cause the motion of these composite nanotubes under magnetic field, providing them with guided mobility. The obtained magnetic anodic alumina nanotubes are completely characterized and their potential to undergo selective and effective functionalization, and stimuli-responsive load release is demonstrated. For this purpose, protease-triggered release of fluorescent molecules loaded inside the magnetic anodic alumina nanotubes (MAANTs) by selective functionalization is performed. The inner walls of the MAANTs were selectively covered with protein padding of albumin-fluorescein isothiocyanate conjugate (FITC-BSA) through means of silanization. Protein functionalization was designed to undergo proteolytic hydrolysis in presence of cathepsin B- protease highly expressed during growth and initial stages of tumor metastasis – in order to cleave peptide bond of albumin and release fluorescent fragments of the protein. Proteolytic reaction with the enzyme is performed under acidic conditions. Presented arrangement is an exemplary combination of functionalities – which are vast – and of value for applications like drug delivery and biosensing applications.
    Thematic Areas: Surfaces, coatings and films Química Nutrição Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Engenharias i Electronic, optical and magnetic materials Economia Colloid and surface chemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, physical Biotecnología Biomaterials Biodiversidade Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: josep.ferre@urv.cat elisabet.xifre@urv.cat lluis.marsal@urv.cat
    Author identifier: 0000-0002-5210-5133 0000-0001-6072-9889 0000-0002-5976-1408
    Record's date: 2024-10-12
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0021979720312984?via%3Dihub
    Funding program: Marti i Franquès COFUND Doctoral Programme
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Journal Of Colloid And Interface Science. 584 236-245
    APA: Domagalski, J T; Xifre-Perez, E; Tabrizi, M A; Ferre-Borrull, J; Marsal, L F (2021). Magnetic nanoparticle decorated anodic alumina nanotubes for fluorescent detection of cathepsin B. Journal Of Colloid And Interface Science, 584(), 236-245. DOI: 10.1016/j.jcis.2020.09.109
    Acronym: MFP
    Article's DOI: 10.1016/j.jcis.2020.09.109
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Funding program action: Marie Skłodowska-Curie Actions - European Union's Horizon 2020 research and innovation programme
    Publication Type: Journal Publications
  • Keywords:

    Biomaterials,Chemistry, Physical,Colloid and Surface Chemistry,Electronic, Optical and Magnetic Materials,Surfaces, Coatings and Films
    Stimuli-responsive release
    Self-assembly
    Magnetic properties
    Cathepsin b
    Anodic alumina nanotubes
    Surfaces, coatings and films
    Química
    Nutrição
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Interdisciplinar
    Geociências
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Electronic, optical and magnetic materials
    Economia
    Colloid and surface chemistry
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, physical
    Biotecnología
    Biomaterials
    Biodiversidade
    Astronomia / física
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