Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Positional Enrichment by Proton Analysis (PEPA): A One?Dimensional 1H?NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics

  • Identification data

    Identifier: imarina:9011166
    Handle: https://hdl.handle.net/20.500.11797/imarina9011166
    Authors:
    Maria VinaixaMiguel A RodríguezSuvi AivioJordi CapelladesJosep GómezNicolau CanyellasTravis H StrackerOscar Yanes
    Abstract:
    A novel metabolomics approach for NMR?based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C?satellite peaks using 1D?1H?NMR spectra. In comparison with 13C?NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of 13C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment. Altogether, PEPA provides a novel framework for extending the high?throughput of 1H?NMR metabolic profiling to stable isotope tracing in metabolomics, facilitating and complementing the information derived from 2D?NMR experiments and expanding the range of isotopically enriched metabolites detected in cellular extracts.

  • Others:

    Author, as appears in the article.: Maria Vinaixa; Miguel A Rodríguez; Suvi Aivio; Jordi Capellades; Josep Gómez; Nicolau Canyellas; Travis H Stracker; Oscar Yanes
    Department: Bioquímica i Biotecnologia Enginyeria Electrònica, Elèctrica i Automàtica
    URV's Author/s: Cañellas Alberich, Nicolau / Gómez Alvarez, Josep / Rodríguez Gómez, Miquel Àngel / Vinaixa Crevillent, Maria / Yanes Torrado, Óscar
    Abstract: A novel metabolomics approach for NMR?based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C?satellite peaks using 1D?1H?NMR spectra. In comparison with 13C?NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of 13C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment. Altogether, PEPA provides a novel framework for extending the high?throughput of 1H?NMR metabolic profiling to stable isotope tracing in metabolomics, facilitating and complementing the information derived from 2D?NMR experiments and expanding the range of isotopically enriched metabolites detected in cellular extracts.
    Thematic Areas: Química Ciências biológicas iii Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: maria.vinaixa@urv.cat miquelangel.rodriguez@alumni.urv.cat nicolau.canyellas@urv.cat oscar.yanes@urv.cat josep.gomez@urv.cat
    Author identifier: 0000-0001-9804-0171 0000-0003-4856-8132 0000-0003-3695-7157 0000-0002-0573-7621
    Record's date: 2023-09-05
    Papper version: info:eu-repo/semantics/publishedVersion
    Papper original source: Angewandte Chemie. 129 (13): 3585-3589
    APA: Maria Vinaixa; Miguel A Rodríguez; Suvi Aivio; Jordi Capellades; Josep Gómez; Nicolau Canyellas; Travis H Stracker; Oscar Yanes (2017). Positional Enrichment by Proton Analysis (PEPA): A One?Dimensional 1H?NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics. Angewandte Chemie, 129(13), 3585-3589. DOI: 10.1002/ange.201611347
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    Publication Type: Journal Publications

  • Keywords:

    metabolism; metabolomics; NMR spectroscopy; stable isotopes
    Química
    Ciências biológicas iii
    Astronomia / física

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