Author, as appears in the article.: Gibert-Ramos, Albert; Martin-Gonzalez, Miguel Z.; Crescenti, Anna; Salvado, M. Josepa;

Department: Bioquímica i Biotecnologia

e-ISSN: 2072-6643

URV's Author/s: GIBERT RAMOS, ALBERT / Salvadó Rovira, Maria Josepa

Keywords: White adipose tissue Weight-loss Uncoupling protein-1 Supplementation Procyanidins Polyphenol Obesity Lipase expression Increases Gspe Exercise Conjugated linoleic-acid Conjugated linoleic acid Anthocyanin Adipocyte

Abstract: Scientists are focusing on bioactive ingredients to counteract obesity. We evaluated whether a mix containing grape seed proanthocyanidin extract (GSPE), anthocyanins, conjugated linoleic acid (CLA), and chicken feet hydrolysate (CFH) could reduce body fat mass and also determined which mechanisms in the white adipose tissue (WAT) and the brown adipose tissue (BAT) were affected by the treatment. The mix or vehicle (VH) were administered for three weeks to obese rats fed a cafeteria (CAF) diet. Biometric measures, indirect calorimetry, and gene expression in WAT and BAT were analyzed as was the histology of the inguinal WAT (IWAT). The individual compounds were also tested in the 3T3-L1 cell line. The mix treatment resulted in a significant 15% reduction in fat (25.01 +/- 0.91 g) compared to VH treatment (21.19 +/- 1.59 g), and the calorimetry results indicated a significant increase in energy expenditure and fat oxidation. We observed a significant downregulation of Fasn mRNA and an upregulation of Atgl and Hsl mRNA in adipose depots in the group treated with the mix. The IWAT showed a tendency of reduction in the number of adipocytes, although no differences in the total adipocyte area were found. GSPE and anthocyanins modulated the lipid content and downregulated the gene and protein levels of Fasn compared to the untreated group in 3T3-L1 cells. In conclusion, this mix is a promising treatment against obesity, reducing the WAT of obese rats fed a CAF diet, increasing energy expenditure and fat oxidation, and modifying the expression of genes involved in lipid metabolism of the adipose tissue.

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Food science Farmacia Engenharias iv Engenharias ii Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: mariajosepa.salvado@urv.cat

Author identifier: 0000-0003-3883-3326

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2072-6643/12/11/3251

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Nutrients. 12 (11):

APA: Gibert-Ramos, Albert; Martin-Gonzalez, Miguel Z.; Crescenti, Anna; Salvado, M. Josepa; (2020). A Mix of Natural Bioactive Compounds Reduces Fat Accumulation and Modulates Gene Expression in the Adipose Tissue of Obese Rats Fed a Cafeteria Diet. Nutrients, 12(11), -. DOI: 10.3390/nu12113251

Article's DOI: 10.3390/nu12113251

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications