Articles producció científica> Química Física i Inorgànica

Structural recognition of triple-stranded DNA by surface-enhanced raman spectroscopy

  • Identification data

    Identifier: imarina:9150163
    Authors:
    Guerrini LAlvarez-Puebla RA
    Abstract:
    © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Direct, label-free analysis of nucleic acids via surface-enhanced Raman spectroscopy (SERS) has been continuously expanding its range of applications as an intriguing and powerful analytical tool for the structural characterization of diverse DNA structures. Still, interrogation of nucleic acid tertiary structures beyond the canonical double helix often remains challenging. In this work, we report for the first time the structural identification of DNA triplex structures. This class of nucleic acids has been attracting great interest because of their intriguing biological functions and pharmacological potential in gene therapy, and the ability for precisely engineering DNA-based functional nanomaterials. Herein, structural discrimination of the triplex structure against its du-plex and tertiary strand counterparts is univocally revealed by recognizing key markers bands in the intrinsic SERS fingerprint. These vibrational features are informative of the base stacking, Hoogsteen hydrogen bonding and sugar–phosphate backbone reorganization associated with the triple helix formation. This work expands the applicability of direct SERS to nucleic acids analysis, with potential impact on fields such as sensing, biology and drug design.
  • Others:

    Author, as appears in the article.: Guerrini L; Alvarez-Puebla RA
    Department: Química Física i Inorgànica
    e-ISSN: 2079-4991
    URV's Author/s: Alvarez Puebla, Ramon Angel / Guerrini, Luca
    Keywords: Triplex Surface-enhanced raman spectroscopy Plasmonic nanoparticles Dna
    Abstract: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Direct, label-free analysis of nucleic acids via surface-enhanced Raman spectroscopy (SERS) has been continuously expanding its range of applications as an intriguing and powerful analytical tool for the structural characterization of diverse DNA structures. Still, interrogation of nucleic acid tertiary structures beyond the canonical double helix often remains challenging. In this work, we report for the first time the structural identification of DNA triplex structures. This class of nucleic acids has been attracting great interest because of their intriguing biological functions and pharmacological potential in gene therapy, and the ability for precisely engineering DNA-based functional nanomaterials. Herein, structural discrimination of the triplex structure against its du-plex and tertiary strand counterparts is univocally revealed by recognizing key markers bands in the intrinsic SERS fingerprint. These vibrational features are informative of the base stacking, Hoogsteen hydrogen bonding and sugar–phosphate backbone reorganization associated with the triple helix formation. This work expands the applicability of direct SERS to nucleic acids analysis, with potential impact on fields such as sensing, biology and drug design.
    Thematic Areas: Physics, applied Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General chemical engineering Engenharias ii Chemistry, multidisciplinary Chemical engineering (miscellaneous) Chemical engineering (all)
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: ramon.alvarez@urv.cat luca.guerrini@urv.cat
    Author identifier: 0000-0003-4770-5756 0000-0002-2925-1562
    Record's date: 2024-07-27
    Journal volume: 11
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2079-4991/11/2/326
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Nanomaterials. 11 (2): 1-9
    APA: Guerrini L; Alvarez-Puebla RA (2021). Structural recognition of triple-stranded DNA by surface-enhanced raman spectroscopy. Nanomaterials, 11(2), 1-9. DOI: 10.3390/nano11020326
    Article's DOI: 10.3390/nano11020326
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Chemical Engineering (Miscellaneous),Chemistry, Multidisciplinary,Materials Science (Miscellaneous),Materials Science, Multidisciplinary,Nanoscience & Nanotechnology,Physics, Applied
    Triplex
    Surface-enhanced raman spectroscopy
    Plasmonic nanoparticles
    Dna
    Physics, applied
    Nanoscience & nanotechnology
    Materials science, multidisciplinary
    Materials science (miscellaneous)
    Materials science (all)
    General materials science
    General chemical engineering
    Engenharias ii
    Chemistry, multidisciplinary
    Chemical engineering (miscellaneous)
    Chemical engineering (all)
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