Author, as appears in the article.: Rodríguez-Calvo, R; Guardiola, M; Oliva, I; Arrando, H; Arranz, I; Ferré, A; Pellicer, P; Parra, S; Ribalta, J; Castro, A
Department: Medicina i Cirurgia
URV's Author/s: Arranz De La Calle, Isabel / Castro Salomó, Antoni / Guardiola Guionnet, Montserrat / Oliva Rodríguez, Iris / Parra Pérez, Sandra / Ribalta Vives, Josep / Rodríguez Calvo, Ricardo
Keywords: Systemic-lupus-erythematosus Sle Low-density lipoprotein Endothelial cells Cardiovascular disease subclasses risk low-density lipoprotein endothelial cells coronary-heart-disease cardiovascular disease accelerated atherosclerosis
Abstract: © The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For permissions, please email: journals.permissions@oup.com. OBJECTIVES: SLE patients have an enhanced risk of atherosclerosis and cardiovascular disease. However, the increased prevalence of cardiovascular disease is not fully explained by traditional Framingham cardiovascular risk factors. Specific features of low-density lipoprotein (LDL) particles, other than plasma concentration, may induce accelerated atherosclerosis at early stages in these patients. Thus, we aimed to explore the impact of LDL from both active and inactive SLE patients on human aortic endothelial cells. METHODS: Human aortic endothelial cells were stimulated with the same concentration of LDL particles isolated from pooled serum that was collected from 13 SLE patients during both active and inactive states. Gene expression and cell migration assays were performed. RESULTS: Circulating LDL particles obtained from healthy volunteers and SLE patients in both remission and flare states were comparable in terms of number, cholesterol and triglyceride content, and net electric charge. Stimulation of cells with LDL from active SLE patients induced the expression of vascular cell adhesion molecule 1 (∼2.0-fold, P < 0.05), monocyte chemoattractant protein 1 (∼2.0-fold, P < 0.05) and matrix metallopeptidase 2 (∼1.6-fold, P < 0.01) compared with cells stimulated with LDL from inactive SLE patients. Additionally, LDL extracted from active patients increased cell migration in a wound-healing assay (1.4-fold, P < 0.05). CONCLUSION: Our data show that, at the same LDL concentration, LDL from active SLE patients had increased proatherogenic effects on endothelial cells compared with LDL from the same patients when in an inactive or remission state.
Thematic Areas: Saúde coletiva Rheumatology Pharmacology (medical) Odontología Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Ensino Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: ricardo.rodriguez@urv.cat isabel.arranz@estudiants.urv.cat sandra.parra@urv.cat antoni.castro@urv.cat montse.guardiola@urv.cat josep.ribalta@urv.cat
Author identifier: 0000-0001-9363-6574 0000-0001-5441-6333 0000-0002-9696-7384 0000-0002-8879-4719
Record's date: 2024-07-27
Papper version: info:eu-repo/semantics/submittedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Rheumatology. 60 (2): 866-871
APA: Rodríguez-Calvo, R; Guardiola, M; Oliva, I; Arrando, H; Arranz, I; Ferré, A; Pellicer, P; Parra, S; Ribalta, J; Castro, A (2021). Low-density lipoprotein from active SLE patients is more atherogenic to endothelial cells than low-density lipoprotein from the same patients during remission. Rheumatology, 60(2), 866-871. DOI: 10.1093/rheumatology/keaa380
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV