Articles producció científica> Bioquímica i Biotecnologia

Impact of gut microbiota on plasma oxylipins profile under healthy and obesogenic conditions

  • Identification data

    Identifier: imarina:9173456
    Authors:
    Ávila-Román JArreaza-Gil VCortés-Espinar AJSoliz-Rueda JRMulero MMuguerza BArola-Arnal AArola LTorres-Fuentes C
    Abstract:
    © 2021 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism Background & aims: Oxylipins (OXLs) are bioactive lipid metabolites derived from polyunsaturated fatty acids (PUFAs) which act as signaling molecules and are involved in inflammatory processes such as those that occur in obesity. On the other hand, gut microbiota plays an essential role in regulating inflammatory responses. However, little is known about the potential impact of gut bacteria on OXLs metabolism. Thus, the objective of this study was to investigate the effect of gut microbiota dysbiosis on plasma oxylipins profile in healthy and diet-induced obese animals. Methods: Eight-week-old male Wistar rats were fed with either a standard or cafeteria diet (CAF) for 5 weeks and administered an antibiotic cocktail (ABX) in the drinking water (Ampicillin: 1 g/ml, Vancomycin: 0.5 g/ml, Imipenem: 0.25 g/ml) for the last 2 weeks in order to induce gut microbiota dysbiosis. Metabolomics analysis of OXLs in plasma was performed by HPLC–MS analysis. No antibiotic treated animals were included as controls. Results: Plasma OXLs profile was significantly altered due to both CAF feeding and ABX administration. ABX effect was more pronounced under obesogenic conditions. Several significant correlations between different bacteria taxa and these lipid mediators were observed. Among these, the positive correlation of Proteobacteria with LTB4, a proinflammatory OXL involved in obesity-related disorders, was especially remarkable. Conclusions: Gut microbiota plays a key role in regulating these lipid metabolites and, therefore, affecting oxylipins-mediated inflammatory processes. These results are the first evidence to our knowledge of gut microbiota impact on OXLs metabolism. Moreover, this can set the bas
  • Others:

    Author, as appears in the article.: Ávila-Román J; Arreaza-Gil V; Cortés-Espinar AJ; Soliz-Rueda JR; Mulero M; Muguerza B; Arola-Arnal A; Arola L; Torres-Fuentes C
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Arola Arnal, Anna / Arola Ferrer, Luis Maria / Arreaza Gil, Verónica / Ávila Román, Francisco Javier / Cortés Espinar, Antonio Jesús / Muguerza Marquínez, Maria Begoña / Mulero Abellán, Miguel / Soliz Rueda, Jorge Ricardo / Torres Fuentes, Cristina
    Keywords: Resolution Oxylipins Obesity Metabolic syndrome Mechanisms Lipid mediators Inflammation Gut microbiota Germ-free Dietary linoleic-acid Cafeteria diet Antibiotics Accumulation
    Abstract: © 2021 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism Background & aims: Oxylipins (OXLs) are bioactive lipid metabolites derived from polyunsaturated fatty acids (PUFAs) which act as signaling molecules and are involved in inflammatory processes such as those that occur in obesity. On the other hand, gut microbiota plays an essential role in regulating inflammatory responses. However, little is known about the potential impact of gut bacteria on OXLs metabolism. Thus, the objective of this study was to investigate the effect of gut microbiota dysbiosis on plasma oxylipins profile in healthy and diet-induced obese animals. Methods: Eight-week-old male Wistar rats were fed with either a standard or cafeteria diet (CAF) for 5 weeks and administered an antibiotic cocktail (ABX) in the drinking water (Ampicillin: 1 g/ml, Vancomycin: 0.5 g/ml, Imipenem: 0.25 g/ml) for the last 2 weeks in order to induce gut microbiota dysbiosis. Metabolomics analysis of OXLs in plasma was performed by HPLC–MS analysis. No antibiotic treated animals were included as controls. Results: Plasma OXLs profile was significantly altered due to both CAF feeding and ABX administration. ABX effect was more pronounced under obesogenic conditions. Several significant correlations between different bacteria taxa and these lipid mediators were observed. Among these, the positive correlation of Proteobacteria with LTB4, a proinflammatory OXL involved in obesity-related disorders, was especially remarkable. Conclusions: Gut microbiota plays a key role in regulating these lipid metabolites and, therefore, affecting oxylipins-mediated inflammatory processes. These results are the first evidence to our knowledge of gut microbiota impact on OXLs metabolism. Moreover, this can set the basis for developing new obesity markers based on OXLs and gut microbiota profiles.
    Thematic Areas: Saúde coletiva Química Odontología Nutrition and dietetics Nutrition & dietetics Nutrição Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Educação física Critical care and intensive care medicine Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: antoniojesus.cortes@urv.cat franciscojavier.avila@urv.cat jorgericardo.soliz@urv.cat antoniojesus.cortes@urv.cat antoniojesus.cortes@urv.cat veronica.arreaza@urv.cat veronica.arreaza@urv.cat veronica.arreaza@urv.cat miquel.mulero@urv.cat anna.arola@urv.cat lluis.arola@urv.cat begona.muguerza@urv.cat cristina.torres@urv.cat
    Author identifier: 0000-0001-9766-8178 0000-0002-8301-2095 0000-0002-8301-2095 0000-0002-8301-2095 0000-0001-6529-1345 0000-0003-2767-1974 0000-0001-7384-8588 0000-0002-2917-6910
    Record's date: 2024-09-21
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.clinicalnutritionjournal.com/article/S0261-5614(21)00123-0/fulltext#%20
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Clinical Nutrition. 40 (4): 1475-1486
    APA: Ávila-Román J; Arreaza-Gil V; Cortés-Espinar AJ; Soliz-Rueda JR; Mulero M; Muguerza B; Arola-Arnal A; Arola L; Torres-Fuentes C (2021). Impact of gut microbiota on plasma oxylipins profile under healthy and obesogenic conditions. Clinical Nutrition, 40(4), 1475-1486. DOI: 10.1016/j.clnu.2021.02.035
    Article's DOI: 10.1016/j.clnu.2021.02.035
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Critical Care and Intensive Care Medicine,Nutrition & Dietetics,Nutrition and Dietetics
    Resolution
    Oxylipins
    Obesity
    Metabolic syndrome
    Mechanisms
    Lipid mediators
    Inflammation
    Gut microbiota
    Germ-free
    Dietary linoleic-acid
    Cafeteria diet
    Antibiotics
    Accumulation
    Saúde coletiva
    Química
    Odontología
    Nutrition and dietetics
    Nutrition & dietetics
    Nutrição
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    General medicine
    Farmacia
    Engenharias iv
    Enfermagem
    Educação física
    Critical care and intensive care medicine
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciência de alimentos
    Biotecnología
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