Author, as appears in the article.: Ávila-Román J; Arreaza-Gil V; Cortés-Espinar AJ; Soliz-Rueda JR; Mulero M; Muguerza B; Arola-Arnal A; Arola L; Torres-Fuentes C
Department: Bioquímica i Biotecnologia
URV's Author/s: Arola Arnal, Anna / Arola Ferrer, Luis Maria / Arreaza Gil, Verónica / Ávila Román, Francisco Javier / Cortés Espinar, Antonio Jesús / Muguerza Marquínez, Maria Begoña / Mulero Abellán, Miguel / Soliz Rueda, Jorge Ricardo / Torres Fuentes, Cristina
Keywords: Resolution Oxylipins Obesity Metabolic syndrome Mechanisms Lipid mediators Inflammation Gut microbiota Germ-free Dietary linoleic-acid Cafeteria diet Antibiotics Accumulation
Abstract: © 2021 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism Background & aims: Oxylipins (OXLs) are bioactive lipid metabolites derived from polyunsaturated fatty acids (PUFAs) which act as signaling molecules and are involved in inflammatory processes such as those that occur in obesity. On the other hand, gut microbiota plays an essential role in regulating inflammatory responses. However, little is known about the potential impact of gut bacteria on OXLs metabolism. Thus, the objective of this study was to investigate the effect of gut microbiota dysbiosis on plasma oxylipins profile in healthy and diet-induced obese animals. Methods: Eight-week-old male Wistar rats were fed with either a standard or cafeteria diet (CAF) for 5 weeks and administered an antibiotic cocktail (ABX) in the drinking water (Ampicillin: 1 g/ml, Vancomycin: 0.5 g/ml, Imipenem: 0.25 g/ml) for the last 2 weeks in order to induce gut microbiota dysbiosis. Metabolomics analysis of OXLs in plasma was performed by HPLC–MS analysis. No antibiotic treated animals were included as controls. Results: Plasma OXLs profile was significantly altered due to both CAF feeding and ABX administration. ABX effect was more pronounced under obesogenic conditions. Several significant correlations between different bacteria taxa and these lipid mediators were observed. Among these, the positive correlation of Proteobacteria with LTB4, a proinflammatory OXL involved in obesity-related disorders, was especially remarkable. Conclusions: Gut microbiota plays a key role in regulating these lipid metabolites and, therefore, affecting oxylipins-mediated inflammatory processes. These results are the first evidence to our knowledge of gut microbiota impact on OXLs metabolism. Moreover, this can set the basis for developing new obesity markers based on OXLs and gut microbiota profiles.
Thematic Areas: Saúde coletiva Química Odontología Nutrition and dietetics Nutrition & dietetics Nutrição Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Educação física Critical care and intensive care medicine Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: franciscojavier.avila@urv.cat cristina.torres@urv.cat begona.muguerza@urv.cat lluis.arola@urv.cat anna.arola@urv.cat miquel.mulero@urv.cat veronica.arreaza@estudiants.urv.cat veronica.arreaza@estudiants.urv.cat antoniojesus.cortes@urv.cat antoniojesus.cortes@urv.cat jorgericardo.soliz@urv.cat
Author identifier: 0000-0001-9766-8178 0000-0002-2917-6910 0000-0001-7384-8588 0000-0003-2767-1974 0000-0001-6529-1345 0000-0002-8301-2095 0000-0002-8301-2095
Record's date: 2024-05-23
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.clinicalnutritionjournal.com/article/S0261-5614(21)00123-0/fulltext#%20
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Clinical Nutrition. 40 (4): 1475-1486
APA: Ávila-Román J; Arreaza-Gil V; Cortés-Espinar AJ; Soliz-Rueda JR; Mulero M; Muguerza B; Arola-Arnal A; Arola L; Torres-Fuentes C (2021). Impact of gut microbiota on plasma oxylipins profile under healthy and obesogenic conditions. Clinical Nutrition, 40(4), 1475-1486. DOI: 10.1016/j.clnu.2021.02.035
Article's DOI: 10.1016/j.clnu.2021.02.035
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV