Author, as appears in the article.: Gomez, Enrique; Canela, Nuria; Herrero, Pol; Cereto, Adria; Gimeno, Isabel; Carrocera, Susana; Martin-Gonzalez, David; Murillo, Antonio; Munoz, Marta;
Department: Bioquímica i Biotecnologia
URV's Author/s: Cereto Massagué, Adrián José / HERRERO GIL, POL
Keywords: Vitrification Valine Time of flight mass spectrometry Threonine Stearic acid Spermatozoon Recipient Receiver operating characteristic Quadrupole mass spectrometry Prolactin Pregnancy rate Pregnancy Precipitation Palmitic acid Nonhuman Morphology Metabolomics Metabolome Metabolism Lysine Lipid metabolism In vitro oocyte maturation In vitro fertilization In vitro Hydrocinnamic acid Holstein cattle Hippuric acid Heptadecanoic acid Glycerophosphate Glycerol stearate Gas chromatography Female Enzyme linked immunosorbent assay Embryo transfer Embryo culture Embryo Echography Dehydroascorbic acid Decanoic acid Cryopreservation Controlled study Citric acid Cell count Bovine Blastocyst Article Animal experiment Alanine
Abstract: This work describes the use of mass spectrometry-based metabolomics as a non-invasive approach to accurately predict birth prior to embryo transfer (ET) starting from embryo culture media and plasma recipient. Metabolomics was used here as a predictive platform. Day-6 in vitro produced embryos developed singly in modified synthetic oviduct fluid culture medium (CM) drops for 24 h were vitrified as Day-7 blastocysts and transferred to recipients. Day-0 and Day-7 recipient plasma (N = 36 x 2) and CM (N = 36) were analyzed by gas chromatography coupled to the quadrupole time of flight mass spectrometry (GC-qTOF). Metabolites quantified in CM and plasma were analyzed as a function to predict pregnancy at Day-40, Day-62, and birth (univariate and multivariate statistics). Subsequently, a Boolean matrix (F1 score) was constructed with metabolite pairs (one from the embryo, and one from the recipient) to combine the predictive power of embryos and recipients. Validation was performed in independent cohorts of ETs analyzed. Embryos that did not reach birth released more stearic acid, capric acid, palmitic acid, and glyceryl monostearate in CM (i.e., (p < 0.05, FDR < 0.05, Receiver Operator Characteristic-area under curve (ROC-AUC) > 0.669)). Within Holstein recipients, hydrocinnamic acid, alanine, and lysine predicted birth (ROC-AUC > 0.778). Asturiana de los Valles recipients that reached birth showed lower concentrations of 6-methyl-5-hepten-2-one, stearic acid, palmitic acid, and hippuric acid (ROC-AUC > 0.832). Embryonal capric acid and glyceryl-monostearate formed F1 scores generally >0.900, with metabolites found both to differ (e.g., hippuric acid, hydrocinnamic acid) or not (e.g., heptadecanoic acid, citric acid) with pregnancy in plasmas, as hypothesized. Efficient lipid metabolism in the embryo and the recipient can allow pregnancy to proceed. Changes in phenolics from plasma suggest that microbiota and liver metabolism influence the pregnancy establishment in cattle.
Thematic Areas: Molecular biology Medicina ii Farmacia Endocrinology, diabetes and metabolism Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry & molecular biology Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: adrianjose.cereto@urv.cat
Record's date: 2024-07-27
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2218-1989/11/3/162
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Metabolites. 11 (3):
APA: Gomez, Enrique; Canela, Nuria; Herrero, Pol; Cereto, Adria; Gimeno, Isabel; Carrocera, Susana; Martin-Gonzalez, David; Murillo, Antonio; Munoz, Marta; (2021). Metabolites Secreted by Bovine Embryos In Vitro Predict Pregnancies That the Recipient Plasma Metabolome Cannot, and Vice Versa. Metabolites, 11(3), -. DOI: 10.3390/metabo11030162
Article's DOI: 10.3390/metabo11030162
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV