Author, as appears in the article.: Gomez, Enrique; Canela, Nuria; Herrero, Pol; Cereto, Adria; Gimeno, Isabel; Carrocera, Susana; Martin-Gonzalez, David; Murillo, Antonio; Munoz, Marta;
Department: Bioquímica i Biotecnologia
URV's Author/s: Cereto Massagué, Adrián José / HERRERO GIL, POL
Keywords: Vitrification; Valine; Time of flight mass spectrometry; Threonine; Stearic acid; Spermatozoon; Recipient; Receiver operating characteristic; Quadrupole mass spectrometry; Prolactin; Pregnancy rate; Pregnancy; Precipitation; Palmitic acid; Nonhuman; Morphology; Metabolomics; Metabolome; Metabolism; Lysine; Lipid metabolism; In vitro oocyte maturation; In vitro fertilization; In vitro; Hydrocinnamic acid; Holstein cattle; Hippuric acid; Heptadecanoic acid; Glycerophosphate; Glycerol stearate; Gas chromatography; Female; Enzyme linked immunosorbent assay; Embryo transfer; Embryo culture; Embryo; Echography; Dehydroascorbic acid; Decanoic acid; Cryopreservation; Controlled study; Citric acid; Cell count; Bovine; Blastocyst; Article; Animal experiment; Alanine
Abstract: This work describes the use of mass spectrometry-based metabolomics as a non-invasive approach to accurately predict birth prior to embryo transfer (ET) starting from embryo culture media and plasma recipient. Metabolomics was used here as a predictive platform. Day-6 in vitro produced embryos developed singly in modified synthetic oviduct fluid culture medium (CM) drops for 24 h were vitrified as Day-7 blastocysts and transferred to recipients. Day-0 and Day-7 recipient plasma (N = 36 x 2) and CM (N = 36) were analyzed by gas chromatography coupled to the quadrupole time of flight mass spectrometry (GC-qTOF). Metabolites quantified in CM and plasma were analyzed as a function to predict pregnancy at Day-40, Day-62, and birth (univariate and multivariate statistics). Subsequently, a Boolean matrix (F1 score) was constructed with metabolite pairs (one from the embryo, and one from the recipient) to combine the predictive power of embryos and recipients. Validation was performed in independent cohorts of ETs analyzed. Embryos that did not reach birth released more stearic acid, capric acid, palmitic acid, and glyceryl monostearate in CM (i.e., (p < 0.05, FDR < 0.05, Receiver Operator Characteristic-area under curve (ROC-AUC) > 0.669)). Within Holstein recipients, hydrocinnamic acid, alanine, and lysine predicted birth (ROC-AUC > 0.778). Asturiana de los Valles recipients that reached birth showed lower concentrations of 6-methyl-5-hepten-2-one, stearic acid, palmitic acid, and hippuric acid (ROC-AUC > 0.832). Embryonal capric acid and glyceryl-monostearate formed F1 scores generally >0.900, with metabolites found both to differ (e.g., hippuric acid, hydrocinnamic acid) or not (e.g., heptadecanoic acid, citric acid) with pregnancy in plasmas, as hypothesized. Efficient lipid metabolism in the embryo and the recipient can allow pregnancy to proceed. Changes in phenolics from plasma suggest that microbiota and liver metabolism influence the pregnancy establishment in cattle.
Thematic Areas: Molecular biology; Medicina ii; Farmacia; Endocrinology, diabetes and metabolism; Ciências biológicas ii; Ciências biológicas i; Biotecnología; Biochemistry & molecular biology; Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: adrianjose.cereto@urv.cat
Record's date: 2024-07-27
Paper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2218-1989/11/3/162
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Metabolites. 11 (3):
APA: Gomez, Enrique; Canela, Nuria; Herrero, Pol; Cereto, Adria; Gimeno, Isabel; Carrocera, Susana; Martin-Gonzalez, David; Murillo, Antonio; Munoz, Marta; (2021). Metabolites Secreted by Bovine Embryos In Vitro Predict Pregnancies That the Recipient Plasma Metabolome Cannot, and Vice Versa. Metabolites, 11(3), -. DOI: 10.3390/metabo11030162
Article's DOI: 10.3390/metabo11030162
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV