Articles producció científica> Medicina i Cirurgia

APC promoter is frequently methylated in pancreatic juice of patients with pancreatic carcinomas or periampullary tumors

  • Identification data

    Identifier: imarina:9187173
    Authors:
    Ginesta MMDiaz-Riascos ZVBusquets JPelaez NSerrano TPeinado MÀJorba RGarcía-Borobia FJCapella GFabregat J
    Abstract:
    Early detection of pancreatic and periampullary neoplasms is critical to improve their clinical outcome. The present authors previously demonstrated that DNA hypermethylation of adenomatous polyposis coli (APC), histamine receptor H2 (HRH2), cadherin 13 (CDH13), secreted protein acidic and cysteine rich (SPARC) and engrailed-1 (EN-1) promoters is frequently detected in pancreatic tumor cells. The aim of the present study was to assess their prevalence in pancreatic juice of carcinomas of the pancreas and periampullary area. A total of 135 pancreatic juices obtained from 85 pancreatic cancer (PC), 26 ampullary carcinoma (AC), 10 intraductal papillary mucinous neoplasm (IPMN) and 14 chronic pancreatitis (CP) patients were analyzed. The methylation status of the APC, HRH2, CDH13, SPARC and EN-1 promoters was analyzed using methylation specific-melting curve analysis (MS-MCA). Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations were also tested with allele-specific quantitative polymerase chain reaction amplification. Out of the 5 promoters analyzed, APC (71%) and HRH2 (65%) were the most frequently methylated in PC juice. APC methylation was also detected at a high frequency in AC (76%) and IPMN (80%), but only occasionally observed in CP (7%). APC methylation had a high sensitivity (71-80%) for all types of cancer analyzed. The panel (where a sample scored as positive when ≥2 markers were methylated) did not outperform APC as a single marker. Finally, KRAS detection in pancreatic juice offered a lower sensitivity (50%) and specificity (71%) for detection of any cancer. APC hypermethylation in pancreatic juice, as assessed by MS-MCA, is a frequent event of potential clinical usefulness in the diagnosis of pancreatic and periampullary neoplasms.
  • Others:

    Author, as appears in the article.: Ginesta MM; Diaz-Riascos ZV; Busquets J; Pelaez N; Serrano T; Peinado MÀ; Jorba R; García-Borobia FJ; Capella G; Fabregat J
    Department: Medicina i Cirurgia
    e-ISSN: 1792-1082
    URV's Author/s: Jorba Martin, Rosa Maria
    Keywords: Pc Papillary mucinous neoplasms Panel Pancreatic cancer Oncology Mutation detection Multiple genes Molecular pathology Methylation K-ras Ipmn Hypermethylation Dna methylation Diagnosis Cells Cancer genetics Cancer Apc Ac
    Abstract: Early detection of pancreatic and periampullary neoplasms is critical to improve their clinical outcome. The present authors previously demonstrated that DNA hypermethylation of adenomatous polyposis coli (APC), histamine receptor H2 (HRH2), cadherin 13 (CDH13), secreted protein acidic and cysteine rich (SPARC) and engrailed-1 (EN-1) promoters is frequently detected in pancreatic tumor cells. The aim of the present study was to assess their prevalence in pancreatic juice of carcinomas of the pancreas and periampullary area. A total of 135 pancreatic juices obtained from 85 pancreatic cancer (PC), 26 ampullary carcinoma (AC), 10 intraductal papillary mucinous neoplasm (IPMN) and 14 chronic pancreatitis (CP) patients were analyzed. The methylation status of the APC, HRH2, CDH13, SPARC and EN-1 promoters was analyzed using methylation specific-melting curve analysis (MS-MCA). Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations were also tested with allele-specific quantitative polymerase chain reaction amplification. Out of the 5 promoters analyzed, APC (71%) and HRH2 (65%) were the most frequently methylated in PC juice. APC methylation was also detected at a high frequency in AC (76%) and IPMN (80%), but only occasionally observed in CP (7%). APC methylation had a high sensitivity (71-80%) for all types of cancer analyzed. The panel (where a sample scored as positive when ≥2 markers were methylated) did not outperform APC as a single marker. Finally, KRAS detection in pancreatic juice offered a lower sensitivity (50%) and specificity (71%) for detection of any cancer. APC hypermethylation in pancreatic juice, as assessed by MS-MCA, is a frequent event of potential clinical usefulness in the diagnosis of pancreatic and periampullary neoplasms.
    Thematic Areas: Saúde coletiva Oncology Odontología Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Farmacia Engenharias iv Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cancer research Biotecnología Biodiversidade Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1792-1074
    Author's mail: rosamaria.jorba@urv.cat
    Author identifier: 0000-0003-3307-4340
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.spandidos-publications.com/10.3892/ol.2016.4868#
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Oncology Letters. 12 (3): 2210-2216
    APA: Ginesta MM; Diaz-Riascos ZV; Busquets J; Pelaez N; Serrano T; Peinado MÀ; Jorba R; García-Borobia FJ; Capella G; Fabregat J (2016). APC promoter is frequently methylated in pancreatic juice of patients with pancreatic carcinomas or periampullary tumors. Oncology Letters, 12(3), 2210-2216. DOI: 10.3892/ol.2016.4868
    Article's DOI: 10.3892/ol.2016.4868
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    Publication Type: Journal Publications
  • Keywords:

    Cancer Research,Oncology
    Pc
    Papillary mucinous neoplasms
    Panel
    Pancreatic cancer
    Oncology
    Mutation detection
    Multiple genes
    Molecular pathology
    Methylation
    K-ras
    Ipmn
    Hypermethylation
    Dna methylation
    Diagnosis
    Cells
    Cancer genetics
    Cancer
    Apc
    Ac
    Saúde coletiva
    Oncology
    Odontología
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Farmacia
    Engenharias iv
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Cancer research
    Biotecnología
    Biodiversidade
    Astronomia / física
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