Author, as appears in the article.: Ginesta MM; Diaz-Riascos ZV; Busquets J; Pelaez N; Serrano T; Peinado MÀ; Jorba R; García-Borobia FJ; Capella G; Fabregat J

Department: Medicina i Cirurgia

e-ISSN: 1792-1082

URV's Author/s: Jorba Martin, Rosa Maria

Keywords: Pc Papillary mucinous neoplasms Panel Pancreatic cancer Oncology Mutation detection Multiple genes Molecular pathology Methylation K-ras Ipmn Hypermethylation Dna methylation Diagnosis Cells Cancer genetics Cancer Apc Ac

Abstract: Early detection of pancreatic and periampullary neoplasms is critical to improve their clinical outcome. The present authors previously demonstrated that DNA hypermethylation of adenomatous polyposis coli (APC), histamine receptor H2 (HRH2), cadherin 13 (CDH13), secreted protein acidic and cysteine rich (SPARC) and engrailed-1 (EN-1) promoters is frequently detected in pancreatic tumor cells. The aim of the present study was to assess their prevalence in pancreatic juice of carcinomas of the pancreas and periampullary area. A total of 135 pancreatic juices obtained from 85 pancreatic cancer (PC), 26 ampullary carcinoma (AC), 10 intraductal papillary mucinous neoplasm (IPMN) and 14 chronic pancreatitis (CP) patients were analyzed. The methylation status of the APC, HRH2, CDH13, SPARC and EN-1 promoters was analyzed using methylation specific-melting curve analysis (MS-MCA). Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations were also tested with allele-specific quantitative polymerase chain reaction amplification. Out of the 5 promoters analyzed, APC (71%) and HRH2 (65%) were the most frequently methylated in PC juice. APC methylation was also detected at a high frequency in AC (76%) and IPMN (80%), but only occasionally observed in CP (7%). APC methylation had a high sensitivity (71-80%) for all types of cancer analyzed. The panel (where a sample scored as positive when ≥2 markers were methylated) did not outperform APC as a single marker. Finally, KRAS detection in pancreatic juice offered a lower sensitivity (50%) and specificity (71%) for detection of any cancer. APC hypermethylation in pancreatic juice, as assessed by MS-MCA, is a frequent event of potential clinical usefulness in the diagnosis of pancreatic and periampullary neoplasms.

Thematic Areas: Saúde coletiva Oncology Odontología Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Farmacia Engenharias iv Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cancer research Biotecnología Biodiversidade Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1792-1074

Author's mail: rosamaria.jorba@urv.cat

Author identifier: 0000-0003-3307-4340

Record's date: 2023-03-06

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.spandidos-publications.com/10.3892/ol.2016.4868#

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Oncology Letters. 12 (3): 2210-2216

APA: Ginesta MM; Diaz-Riascos ZV; Busquets J; Pelaez N; Serrano T; Peinado MÀ; Jorba R; García-Borobia FJ; Capella G; Fabregat J (2016). APC promoter is frequently methylated in pancreatic juice of patients with pancreatic carcinomas or periampullary tumors. Oncology Letters, 12(3), 2210-2216. DOI: 10.3892/ol.2016.4868

Article's DOI: 10.3892/ol.2016.4868

Entity: Universitat Rovira i Virgili

Journal publication year: 2016

Publication Type: Journal Publications