Author, as appears in the article.: Valenzuela-Lopez, Nicomedes; Teresa Martin-Gomez, M.; Los-Arcos, Ibai; Stchigel, Alberto M.; Guarro, Josep; Cano-Lira, Jose F.;

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Cano Lira, José Francisco / Guarro Artigas, Josep / Stchigel Glikman, Alberto Miguel / VALENZUELA LÓPEZ, NICOMEDES

Keywords: Pleosporales Mycoses Infection Filamentous fungi Ascomycota

Abstract: Human infections by pleosporalean fungi (class Dothideomycetes, phylum Ascomycota) are rarely reported. Because their identification is challenging using morphological characterization, several phylogenetic markers must be sequenced for an accurate identification and taxonomical placement of the isolates. Three isolates of clinical origin were phenotypically characterized, but due to the absence of relevant morphological traits, D1-D2 domains of the 28S nrRNA gene (LSU), the internal transcribed spacer region (ITS) of the nrRNA, and fragments of the RNA polymerase II subunit 2 (rpb2) and translation elongation factor 1-alpha (tef1) genes were sequenced to allow a phylogenetic analysis that would solve their phylogenetic placement. That analysis revealed that these isolates did not match any previously known pleosporalean genera, and they are proposed here as the new fungal genus, Gambiomyces. Unfortunately, the isolates remained sterile, which, consequently, made the morphological description of the reproductive structures impossible. Future studies should try to understand the behaviour of this fungus in nature as well as its characteristics as an opportunistic fungal pathogen. Molecular identification is becoming an essential tool for proper identification of Dothideomycetes of clinical origin. Lay Abstract We describe a new pleosporalen pathogenic fungus, Gambiomyces profunda, found in superficial to deep samples from a human patient. Because all strains remained sterile, the fungus was finally identified following a phylogenetic analysis by using four different molecular markers.

Thematic Areas: Zootecnia / recursos pesqueiros Veterinary sciences Veterinary (miscellaneous) Saúde coletiva Química Odontología Mycology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Infectious diseases Geografía General veterinary Farmacia Engenharias iii Engenharias ii Engenharias i Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: josep.guarro@urv.cat jose.cano@urv.cat albertomiguel.stchigel@urv.cat

Author identifier: 0000-0002-7839-7568 0000-0003-4495-4394 0000-0003-3987-7996

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://academic.oup.com/mmy/article-abstract/59/3/278/5876953?redirectedFrom=fulltext

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Medical Mycology. 59 (3): 278-288

APA: Valenzuela-Lopez, Nicomedes; Teresa Martin-Gomez, M.; Los-Arcos, Ibai; Stchigel, Alberto M.; Guarro, Josep; Cano-Lira, Jose F.; (2021). A new pleosporalean fungus isolated from superficial to deep human clinical specimens. Medical Mycology, 59(3), 278-288. DOI: 10.1093/mmy/myaa055

Article's DOI: 10.1093/mmy/myaa055

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications