Author, as appears in the article.: Dasilva, Gabriel; Lois, Salome; Mendez, Lucia; Miralles-Perez, Bernat; Romeu, Marta; Ramos-Romero, Sara; Torres, Josep L.; Medina, Isabel;
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Miralles Pérez, Bernat / Romeu Ferran, Marta
Keywords: ω 3 lipid mediators ?3 lipid mediators Adipogenesis Adipose tissue Alanine aminotransferase Animal Animal experiment Animal model Animal tissue Animals Antiinflammatory activity Article Autacoid Biological marker Biomarkers Carbohydrate intake Cd59 antigen Cholesterol blood level Chromatography, liquid Cytochrome p450 Decomposition Dha Diet Diet supplementation Dietary intake Down regulation Drug effect Endoplasmic reticulum stress Enzyme activity Enzyme linked immunosorbent assay Epa Fatty acid analysis Fatty-acids Fish oil Fish oils Food intake Gas chromatography Glucose Glucose blood level Homeostasis Inflammation Inflammation mediators Insulin Ketamine Leptin Linoleic acid Lipid metabolism Lipid storage Lipidome Lipidomics Lipotoxicity Lipoxygenase Liquid chromatography Liquid chromatography-mass spectrometry Male Mass spectrometry Metabolic networks and pathways Metabolism Metabolite Nonhuman Obesity Oleic acid Oxidative stress Peripheral blood mononuclear cell Phenotype Prediabetes Procedures Prostaglandin Protein fingerprinting Rat Rats Saturated fatty acid Soybean oil Specialized resolvers Tandem mass spectrometry Transesterification Triacylglycerol Ultra performance liquid chromatography Ultrasound Upregulation Xylazine
Abstract: Adipose tissue is now recognized as an active organ with an important homeostatic function in glucose and lipid metabolism and the development of insulin resistance. The present research investigates the role of lipid mediators and lipid profiling for controlling inflammation and the metabolic normal function of white adipose tissue from rats suffering from diet-induced prediabetes. Additionally, the contribution to the adipose lipidome induced by the consumption of marine omega-3 PUFAs as potential regulators of inflammation is addressed. For that, the effects on the inflammatory response triggered by high-fat high-sucrose (HFHS) diets were studied in male Sprague-Dawley rats. Using SPE-LC-MS/MS-based metabolo-lipidomics, a range of eicosanoids, docosanoids and specialized pro-resolving mediators (SPMs) were measured in white adipose tissue. The inflammatory response occurring in prediabetic adipose tissue was associated with the decomposition of ARA epoxides to ARA-dihydroxides, the reduction of oxo-derivatives and the formation of prostaglandins (PGs). In an attempt to control the inflammatory response initiated, LOX and non-enzymatic oxidation shifted toward the production of the less pro-inflammatory EPA and DHA metabolites rather than the high pro-inflammatory ARA hydroxides. Additionally, the change in LOX activity induced the production of intermediate hydroxides precursors of SPMs as protectins (PDs), resolvins (Rvs) and maresins (MaRs). This compensatory mechanism to achieve the restoration of tissue homeostasis was significantly strengthened through supplementation with fish oils. Increasing proportions of omega-3 PUFAs in adipose tissue significantly stimulated the formation of DHA-epoxides by cytochrome P450, the production of non-enzymatic EPA-metabolites and prompted the activity of 12LOX. Finally, protectin PDX was significantly reduced in the adipose tissue of prediabetic rats and highly enhanced through omega-3 PUFAs supplementation. Taken together, these actively coordinated modifications constitute key mechanisms to restore adipose tissue homeostasis with an important role of lipid mediators. This compensatory mechanism is reinforced through the supplementation of the diet with fish oils with high and balanced contents of EPA and DHA. The study highlights new insides on the targets for effective treatment of incipient diet-induced diabetes and the mechanism underlying the potential anti-inflammatory action of marine lipids.
Thematic Areas: Biodiversidade Biotecnología Ciência de alimentos Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Engenharias ii Engenharias iii Farmacia Immunology Immunology and allergy Interdisciplinar Medicina i Medicina ii Medicina iii Medicina veterinaria Nutrição Odontología Química Saúde coletiva Zootecnia / recursos pesqueiros
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: bernat.miralles@urv.cat marta.romeu@urv.cat bernat.miralles@urv.cat
Author identifier: 0000-0003-1294-7069 0000-0002-2131-1858 0000-0003-1294-7069
Record's date: 2023-02-19
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://pubmed.ncbi.nlm.nih.gov/33968013/
Papper original source: Frontiers In Immunology. 12 (608875):
APA: Dasilva, Gabriel; Lois, Salome; Mendez, Lucia; Miralles-Perez, Bernat; Romeu, Marta; Ramos-Romero, Sara; Torres, Josep L.; Medina, Isabel; (2021). Fish Oil Improves Pathway-Oriented Profiling of Lipid Mediators for Maintaining Metabolic Homeostasis in Adipose Tissue of Prediabetic Rats. Frontiers In Immunology, 12(608875), -. DOI: 10.3389/fimmu.2021.608875
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.3389/fimmu.2021.608875
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV