Articles producció científica> Ciències Mèdiques Bàsiques

Fish Oil Improves Pathway-Oriented Profiling of Lipid Mediators for Maintaining Metabolic Homeostasis in Adipose Tissue of Prediabetic Rats

  • Identification data

    Identifier: imarina:9207252
    Authors:
    Dasilva, GabrielLois, SalomeMendez, LuciaMiralles-Perez, BernatRomeu, MartaRamos-Romero, SaraTorres, Josep L.Medina, Isabel
    Abstract:
    Adipose tissue is now recognized as an active organ with an important homeostatic function in glucose and lipid metabolism and the development of insulin resistance. The present research investigates the role of lipid mediators and lipid profiling for controlling inflammation and the metabolic normal function of white adipose tissue from rats suffering from diet-induced prediabetes. Additionally, the contribution to the adipose lipidome induced by the consumption of marine omega-3 PUFAs as potential regulators of inflammation is addressed. For that, the effects on the inflammatory response triggered by high-fat high-sucrose (HFHS) diets were studied in male Sprague-Dawley rats. Using SPE-LC-MS/MS-based metabolo-lipidomics, a range of eicosanoids, docosanoids and specialized pro-resolving mediators (SPMs) were measured in white adipose tissue. The inflammatory response occurring in prediabetic adipose tissue was associated with the decomposition of ARA epoxides to ARA-dihydroxides, the reduction of oxo-derivatives and the formation of prostaglandins (PGs). In an attempt to control the inflammatory response initiated, LOX and non-enzymatic oxidation shifted toward the production of the less pro-inflammatory EPA and DHA metabolites rather than the high pro-inflammatory ARA hydroxides. Additionally, the change in LOX activity induced the production of intermediate hydroxides precursors of SPMs as protectins (PDs), resolvins (Rvs) and maresins (MaRs). This compensatory mechanism to achieve the restoration of tissue homeostasis was significantly strengthened through supplementation with fish oils. Increasing proportions of omega-3 PUFAs in adipose tissue significantly stimulated the formation of DHA-epoxides by cytochrome P450, the production of non-enzymatic EPA-metabolites
  • Others:

    Author, as appears in the article.: Dasilva, Gabriel; Lois, Salome; Mendez, Lucia; Miralles-Perez, Bernat; Romeu, Marta; Ramos-Romero, Sara; Torres, Josep L.; Medina, Isabel;
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Miralles Pérez, Bernat / Romeu Ferran, Marta
    Keywords: Xylazine Upregulation Ultrasound Ultra performance liquid chromatography Triacylglycerol Transesterification Tandem mass spectrometry Specialized resolvers Soybean oil Saturated fatty acid Rats Rat Protein fingerprinting Prostaglandin Procedures Prediabetes Phenotype Peripheral blood mononuclear cell Oxidative stress Oleic acid Obesity Nonhuman Metabolite Metabolism Metabolic networks and pathways Mass spectrometry Male Liquid chromatography-mass spectrometry Liquid chromatography Lipoxygenase Lipotoxicity Lipidomics Lipidome Lipid storage Lipid metabolism Linoleic acid Leptin Ketamine Insulin Inflammation mediators Inflammation Homeostasis Glucose blood level Glucose Gas chromatography Food intake Fish oils Fish oil Fatty-acids Fatty acid analysis Epa Enzyme linked immunosorbent assay Enzyme activity Endoplasmic reticulum stress Drug effect Down regulation Dietary intake Diet supplementation Diet Dha Decomposition Cytochrome p450 Chromatography, liquid Cholesterol blood level Cd59 antigen Carbohydrate intake Biomarkers Biological marker Autacoid Article Antiinflammatory activity Animals Animal tissue Animal model Animal experiment Animal Alanine aminotransferase Adipose tissue Adipogenesis ?3 lipid mediators ω 3 lipid mediators
    Abstract: Adipose tissue is now recognized as an active organ with an important homeostatic function in glucose and lipid metabolism and the development of insulin resistance. The present research investigates the role of lipid mediators and lipid profiling for controlling inflammation and the metabolic normal function of white adipose tissue from rats suffering from diet-induced prediabetes. Additionally, the contribution to the adipose lipidome induced by the consumption of marine omega-3 PUFAs as potential regulators of inflammation is addressed. For that, the effects on the inflammatory response triggered by high-fat high-sucrose (HFHS) diets were studied in male Sprague-Dawley rats. Using SPE-LC-MS/MS-based metabolo-lipidomics, a range of eicosanoids, docosanoids and specialized pro-resolving mediators (SPMs) were measured in white adipose tissue. The inflammatory response occurring in prediabetic adipose tissue was associated with the decomposition of ARA epoxides to ARA-dihydroxides, the reduction of oxo-derivatives and the formation of prostaglandins (PGs). In an attempt to control the inflammatory response initiated, LOX and non-enzymatic oxidation shifted toward the production of the less pro-inflammatory EPA and DHA metabolites rather than the high pro-inflammatory ARA hydroxides. Additionally, the change in LOX activity induced the production of intermediate hydroxides precursors of SPMs as protectins (PDs), resolvins (Rvs) and maresins (MaRs). This compensatory mechanism to achieve the restoration of tissue homeostasis was significantly strengthened through supplementation with fish oils. Increasing proportions of omega-3 PUFAs in adipose tissue significantly stimulated the formation of DHA-epoxides by cytochrome P450, the production of non-enzymatic EPA-metabolites and prompted the activity of 12LOX. Finally, protectin PDX was significantly reduced in the adipose tissue of prediabetic rats and highly enhanced through omega-3 PUFAs supplementation. Taken together, these actively coordinated modifications constitute key mechanisms to restore adipose tissue homeostasis with an important role of lipid mediators. This compensatory mechanism is reinforced through the supplementation of the diet with fish oils with high and balanced contents of EPA and DHA. The study highlights new insides on the targets for effective treatment of incipient diet-induced diabetes and the mechanism underlying the potential anti-inflammatory action of marine lipids.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Immunology and allergy Immunology Farmacia Engenharias iii Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología Biodiversidade
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: bernat.miralles@urv.cat marta.romeu@urv.cat
    Author identifier: 0000-0003-1294-7069 0000-0002-2131-1858
    Record's date: 2024-07-27
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://pubmed.ncbi.nlm.nih.gov/33968013/
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Frontiers In Immunology. 12 (608875):
    APA: Dasilva, Gabriel; Lois, Salome; Mendez, Lucia; Miralles-Perez, Bernat; Romeu, Marta; Ramos-Romero, Sara; Torres, Josep L.; Medina, Isabel; (2021). Fish Oil Improves Pathway-Oriented Profiling of Lipid Mediators for Maintaining Metabolic Homeostasis in Adipose Tissue of Prediabetic Rats. Frontiers In Immunology, 12(608875), -. DOI: 10.3389/fimmu.2021.608875
    Article's DOI: 10.3389/fimmu.2021.608875
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Immunology,Immunology and Allergy
    Xylazine
    Upregulation
    Ultrasound
    Ultra performance liquid chromatography
    Triacylglycerol
    Transesterification
    Tandem mass spectrometry
    Specialized resolvers
    Soybean oil
    Saturated fatty acid
    Rats
    Rat
    Protein fingerprinting
    Prostaglandin
    Procedures
    Prediabetes
    Phenotype
    Peripheral blood mononuclear cell
    Oxidative stress
    Oleic acid
    Obesity
    Nonhuman
    Metabolite
    Metabolism
    Metabolic networks and pathways
    Mass spectrometry
    Male
    Liquid chromatography-mass spectrometry
    Liquid chromatography
    Lipoxygenase
    Lipotoxicity
    Lipidomics
    Lipidome
    Lipid storage
    Lipid metabolism
    Linoleic acid
    Leptin
    Ketamine
    Insulin
    Inflammation mediators
    Inflammation
    Homeostasis
    Glucose blood level
    Glucose
    Gas chromatography
    Food intake
    Fish oils
    Fish oil
    Fatty-acids
    Fatty acid analysis
    Epa
    Enzyme linked immunosorbent assay
    Enzyme activity
    Endoplasmic reticulum stress
    Drug effect
    Down regulation
    Dietary intake
    Diet supplementation
    Diet
    Dha
    Decomposition
    Cytochrome p450
    Chromatography, liquid
    Cholesterol blood level
    Cd59 antigen
    Carbohydrate intake
    Biomarkers
    Biological marker
    Autacoid
    Article
    Antiinflammatory activity
    Animals
    Animal tissue
    Animal model
    Animal experiment
    Animal
    Alanine aminotransferase
    Adipose tissue
    Adipogenesis
    ?3 lipid mediators
    ω 3 lipid mediators
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Odontología
    Nutrição
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Immunology and allergy
    Immunology
    Farmacia
    Engenharias iii
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciência de alimentos
    Biotecnología
    Biodiversidade
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