Articles producció científica> Medicina i Cirurgia

The potential protective role of runx1 in nonalcoholic fatty liver disease

  • Identification data

    Identifier: imarina:9216063
    Authors:
    Bertran, LaiaPastor, AngelaPortillo-Carrasquer, MartaBinetti, JessicaAguilar, CarmenMartinez, SalomeVives, MargaritaSabench, FatimaPorras, Jose AntonioRiesco, DavidDel Castillo, DanielRichart, CristobalAuguet, Teresa
    Abstract:
    The pathogenic mechanisms underlying nonalcoholic fatty liver disease (NAFLD) are beginning to be understood. RUNX1 is involved in angiogenesis, which is crucial in inflammation, but its role in nonalcoholic steatohepatitis (NASH) remains unclear. The aim of this study was to analyze RUNX1 mRNA hepatic and jejunal abundance in women with morbid obesity (MO) and NAFLD. RUNX1, lipid metabolism-related genes, and TLRs in women with MO and normal liver (NL, n = 28), NAFLD (n = 41) (simple steatosis (SS, n = 24), or NASH (n = 17)) were analyzed by RT-qPCR. The RUNX1 hepatic expression was higher in SS than in NL or NASH, as likewise confirmed by immunohistochemistry. An increased expression of hepatic FAS was found in NAFLD. Hepatic RUNX1 correlated positively with FAS. There were no significant differences in the jejunum RUNX1 expressions in the different groups. Jejunal FXR expression was lower in NASH than in NL, while the TLR9 expression increased as NAFLD progressed. Jejunal RUNX1 correlated positively with jejunal PPARγ, TLR4, and TLR5. In summary, the hepatic expression of RUNX1 seems to be involved in the first steps of the NAFLD process; however, in NASH, it seems to be downregulated. Our findings provide important insights into the role of RUNX1 in the context of NAFLD/NASH, suggesting a protective role.
  • Others:

    Author, as appears in the article.: Bertran, Laia; Pastor, Angela; Portillo-Carrasquer, Marta; Binetti, Jessica; Aguilar, Carmen; Martinez, Salome; Vives, Margarita; Sabench, Fatima; Porras, Jose Antonio; Riesco, David; Del Castillo, Daniel; Richart, Cristobal; Auguet, Teresa
    Department: Medicina i Cirurgia
    URV's Author/s: Aguilar Crespillo, Carmen Isabel / Auguet Quintillà, Maria Teresa / Bertran Ramos, Laia / Binetti, Jessica Angela / Del Castillo Déjardin, Daniel / Martínez González, María Salomé / Pastor Alcaraz, Ana / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Riesco Acevedo, David Gerardo / Sabench Pereferrer, Fàtima / VIVES GAR
    Keywords: Transcriptome Toll-like receptors Toll-like receptor 9 Tlr9 protein, human Runx1 protein, human Runx1 Rna, messenger Obesity, morbid Non-alcoholic fatty liver disease Nafld Metabolism Liver Lipid metabolism Jejunum Humans Gut–liver axis Female Core binding factor alpha 2 subunit Adult pathophysiology nafld metabolism liver jejunum inflammation gut-liver axis gut microbiota growth-factor gene-expression cells
    Abstract: The pathogenic mechanisms underlying nonalcoholic fatty liver disease (NAFLD) are beginning to be understood. RUNX1 is involved in angiogenesis, which is crucial in inflammation, but its role in nonalcoholic steatohepatitis (NASH) remains unclear. The aim of this study was to analyze RUNX1 mRNA hepatic and jejunal abundance in women with morbid obesity (MO) and NAFLD. RUNX1, lipid metabolism-related genes, and TLRs in women with MO and normal liver (NL, n = 28), NAFLD (n = 41) (simple steatosis (SS, n = 24), or NASH (n = 17)) were analyzed by RT-qPCR. The RUNX1 hepatic expression was higher in SS than in NL or NASH, as likewise confirmed by immunohistochemistry. An increased expression of hepatic FAS was found in NAFLD. Hepatic RUNX1 correlated positively with FAS. There were no significant differences in the jejunum RUNX1 expressions in the different groups. Jejunal FXR expression was lower in NASH than in NL, while the TLR9 expression increased as NAFLD progressed. Jejunal RUNX1 correlated positively with jejunal PPARγ, TLR4, and TLR5. In summary, the hepatic expression of RUNX1 seems to be involved in the first steps of the NAFLD process; however, in NASH, it seems to be downregulated. Our findings provide important insights into the role of RUNX1 in the context of NAFLD/NASH, suggesting a protective role.
    Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: ana.pastor@urv.cat davidgerardo.riesco@urv.cat joseantonio.porras@urv.cat mariasalome.martinez@urv.cat danieldel.castillo@urv.cat jessicaangela.binetti@estudiants.urv.cat ana.pastor@urv.cat laia.bertranr@estudiants.urv.cat laia.bertranr@estudiants.urv.cat fatima.sabench@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat
    Author identifier: 0000-0001-6418-1822 0000-0001-6185-2889 0000-0003-0456-3102 0000-0001-7024-7824 0000-0001-9660-7752 0000-0001-9052-1368 0000-0001-9052-1368 0000-0002-9262-8756 0000-0002-4440-562X 0000-0002-4440-562X 0000-0003-0396-6428
    Record's date: 2024-09-28
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/1422-0067/22/10/5239
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: International Journal Of Molecular Sciences. 22 (10): 5239-
    APA: Bertran, Laia; Pastor, Angela; Portillo-Carrasquer, Marta; Binetti, Jessica; Aguilar, Carmen; Martinez, Salome; Vives, Margarita; Sabench, Fatima; Por (2021). The potential protective role of runx1 in nonalcoholic fatty liver disease. International Journal Of Molecular Sciences, 22(10), 5239-. DOI: 10.3390/ijms22105239
    Article's DOI: 10.3390/ijms22105239
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry & Molecular Biology,Catalysis,Chemistry, Multidisciplinary,Computer Science Applications,Inorganic Chemistry,Medicine (Miscellaneous),Molecular Biology,Organic Chemistry,Physical and Theoretical Chemistry,Spectroscopy
    Transcriptome
    Toll-like receptors
    Toll-like receptor 9
    Tlr9 protein, human
    Runx1 protein, human
    Runx1
    Rna, messenger
    Obesity, morbid
    Non-alcoholic fatty liver disease
    Nafld
    Metabolism
    Liver
    Lipid metabolism
    Jejunum
    Humans
    Gut–liver axis
    Female
    Core binding factor alpha 2 subunit
    Adult
    pathophysiology
    nafld
    metabolism
    liver
    jejunum
    inflammation
    gut-liver axis
    gut microbiota
    growth-factor
    gene-expression
    cells
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Psicología
    Physical and theoretical chemistry
    Organic chemistry
    Odontología
    Nutrição
    Molecular biology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Inorganic chemistry
    Geociências
    Farmacia
    Engenharias iv
    Engenharias ii
    Engenharias i
    Educação física
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, multidisciplinary
    Catalysis
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Astronomia / física
  • Documents:

  • Cerca a google

    Search to google scholar