Author, as appears in the article.: Bertran L; Pastor A; Portillo-Carrasquer M; Binetti J; Aguilar C; Martínez S; Vives M; Sabench F; Porras JA; Riesco D; Castillo DD; Richart C; Auguet T

Department: Medicina i Cirurgia

URV's Author/s: Aguilar Crespillo, Carmen Isabel / Auguet Quintillà, Maria Teresa / Bertran Ramos, Laia / Binetti, Jessica Angela / Del Castillo Déjardin, Daniel / Martínez González, María Salomé / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima

Keywords: Toll-like receptors Runx1 Nafld Metabolism Liver Jejunum Gut–liver axis pathophysiology nafld metabolism liver jejunum inflammation gut-liver axis gut microbiota growth-factor gene-expression cells

Abstract: The pathogenic mechanisms underlying nonalcoholic fatty liver disease (NAFLD) are beginning to be understood. RUNX1 is involved in angiogenesis, which is crucial in inflammation, but its role in nonalcoholic steatohepatitis (NASH) remains unclear. The aim of this study was to analyze RUNX1 mRNA hepatic and jejunal abundance in women with morbid obesity (MO) and NAFLD. RUNX1, lipid metabolism-related genes, and TLRs in women with MO and normal liver (NL, n = 28), NAFLD (n = 41) (simple steatosis (SS, n = 24), or NASH (n = 17)) were analyzed by RT-qPCR. The RUNX1 hepatic expression was higher in SS than in NL or NASH, as likewise confirmed by immunohistochemistry. An increased expression of hepatic FAS was found in NAFLD. Hepatic RUNX1 correlated positively with FAS. There were no significant differences in the jejunum RUNX1 expressions in the different groups. Jejunal FXR expression was lower in NASH than in NL, while the TLR9 expression increased as NAFLD progressed. Jejunal RUNX1 correlated positively with jejunal PPARγ, TLR4, and TLR5. In summary, the hepatic expression of RUNX1 seems to be involved in the first steps of the NAFLD process; however, in NASH, it seems to be downregulated. Our findings provide important insights into the role of RUNX1 in the context of NAFLD/NASH, suggesting a protective role.

Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: joseantonio.porras@urv.cat danieldel.castillo@urv.cat mariasalome.martinez@urv.cat cristobalmanuel.richart@urv.cat mariateresa.auguet@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat fatima.sabench@urv.cat laia.bertran@urv.cat jessicaangela.binetti@estudiants.urv.cat

Author identifier: 0000-0001-6418-1822 0000-0003-0456-3102 0000-0001-7024-7824 0000-0001-6185-2889 0000-0003-0852-6739 0000-0003-0396-6428 0000-0002-4440-562X 0000-0002-4440-562X 0000-0002-9262-8756 0000-0001-9052-1368 0000-0001-9660-7752

Record's date: 2023-06-24

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/1422-0067/22/10/5239

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: International Journal Of Molecular Sciences. 22 (10):

APA: Bertran L; Pastor A; Portillo-Carrasquer M; Binetti J; Aguilar C; Martínez S; Vives M; Sabench F; Porras JA; Riesco D; Castillo DD; Richart C; Auguet (2021). The potential protective role of runx1 in nonalcoholic fatty liver disease. International Journal Of Molecular Sciences, 22(10), -. DOI: 10.3390/ijms22105239

Article's DOI: 10.3390/ijms22105239

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications