Author, as appears in the article.: Harms K; Surup F; Stadler M; Stchigel AM; Marin‐felix Y

Department: Ciències Mèdiques Bàsiques

URV's Author/s: MARÍN FÉLIX, YASMINA / Stchigel Glikman, Alberto Miguel

Keywords: Sordariales ascomycota Sordariales Secondary metabolites Schizotheciaceae Morinagamyces Morinagadepsin Depsipeptide Cytotoxicity systematics sordariales secondary metabolites schizotheciaceae ribosomal dna reevaluation protein phylogeny performance morinagamyces morinagadepsin lasiosphaeriaceae diversity depsipeptide configuration

Abstract: The new genus Morinagamyces is introduced herein to accommodate the fungus Api-osordaria vermicularis as inferred from a phylogenetic study based on sequences of the internal tran-scribed spacer region (ITS), the nuclear rDNA large subunit (LSU), and partial fragments of riboso-mal polymerase II subunit 2 (rpb2) and β‐tubulin (tub2) genes. Morinagamyces vermicularis was analyzed for the production of secondary metabolites, resulting in the isolation of a new depsipeptide named morinagadepsin (1), and the already known chaetone B (3). While the planar structure of 1 was elucidated by extensive 1D‐ and 2D‐NMR analysis and high‐resolution mass spectrometry, the absolute configuration of the building blocks Ala, Val, and Leu was determined as ‐L by Marfey’s method. The configuration of the 3‐hydroxy‐2‐methyldecanyl unit was assigned as 22R,23R by J‐ based configuration analysis and Mosher’s method after partial hydrolysis of the morinagadepsin to the linear derivative compound 2. Compound 1 showed cytotoxic activity against the mammalian cell lines KB3.1 and L929, but no antimicrobial activity against the fungi and bacteria tested was observed, while 2 was inactive. Compound 3 was weakly cytotoxic against the cell line L929, but did not show any antimicrobial activity.

Thematic Areas: Virology Microbiology (medical) Microbiology

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: albertomiguel.stchigel@urv.cat

Author identifier: 0000-0003-3987-7996

Record's date: 2023-02-18

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2076-2607/9/6/1191

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Microorganisms. 9 (6):

APA: Harms K; Surup F; Stadler M; Stchigel AM; Marin‐felix Y (2021). Morinagadepsin, a depsipeptide from the fungus morinagamyces vermicularis gen. Et comb. nov.. Microorganisms, 9(6), -. DOI: 10.3390/microorganisms9061191

Article's DOI: 10.3390/microorganisms9061191

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications