Author, as appears in the article.: Tomas‐hernandez S; Blanco J; Garcia‐vallvé S; Pujadas G; Ojeda‐montes MJ; Gimeno A; Arola L; Minghetti L; Beltrán‐debón R; Mulero M

Department: Bioquímica i Biotecnologia

URV's Author/s: Arola Ferrer, Luis Maria / Beltrán Debón, Raúl Alejandro / Blanco Pérez, Jordi / Garcia Vallve, Santiago / Mulero Abellán, Miguel / OJEDA MONTES, Mª JOSÉ / Pujadas Anguiano, Gerard / TOMAS HERNÁNDEZ, SARA

Keywords: P38 kinase O‐orsellinaldehyde Nf‐κb Neuro‐inflammation Natural compounds Microglia Jnk Immunomodulation Heme oxygenase-1 Grifola frondosa Astrocytes polysaccharide p38 kinase o-orsellinaldehyde nf-kappa b neuro-inflammation natural compounds microglia lipopolysaccharide jnk inflammatory response immunomodulation health grifola frondosa extract culture brain astrocytes activation

Abstract: In response to foreign or endogenous stimuli, both microglia and astrocytes adopt an activated phenotype that promotes the release of pro‐inflammatory mediators. This inflammatory mechanism, known as neuroinflammation, is essential in the defense against foreign invasion and in normal tissue repair; nevertheless, when constantly activated, this process can become detri-mental through the release of neurotoxic factors that amplify underlying disease. In consequence, this study presents the anti‐inflammatory and immunomodulatory properties of o‐orsellinaldehyde, a natural compound found by an in silico approach in the Grifola frondosa mushroom, in astrocytes and microglia cells. For this purpose, primary microglia and astrocytes were isolated from mice brain and cultured in vitro. Subsequently, cells were exposed to LPS in the absence or presence of increasing concentrations of this natural compound. Specifically, the results shown that o‐orsellinaldehyde strongly inhibits the LPS‐induced inflammatory response in astrocytes and microglia by decreasing nitrite formation and downregulating iNOS and HO‐1 expression. Furthermore, in mi-croglia cells o‐orsellinaldehyde inhibits NF‐κB activation; and potently counteracts LPS‐mediated p38 kinase and JNK phosphorylation (MAPK). In this regard, o‐orsellinaldehyde treatment also induces a significant cell immunomodulation by repolarizing microglia toward the M2 anti‐inflammatory phenotype. Altogether, these results could partially explain the reported beneficial effects of G. frondosa extracts on inflammatory conditions.

Thematic Areas: Pharmacology & pharmacy Pharmaceutical science Medicina ii Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: jordi.blanco@urv.cat raul.beltran@urv.cat lluis.arola@urv.cat gerard.pujadas@urv.cat santi.garcia-vallve@urv.cat miquel.mulero@urv.cat

Author identifier: 0000-0001-8016-0984 0000-0001-9691-1906 0000-0003-2767-1974 0000-0003-2598-8089 0000-0002-0348-7497

Record's date: 2023-02-19

Journal volume: 13

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/1999-4923/13/6/806

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Pharmaceutics. 13 (6):

APA: Tomas‐hernandez S; Blanco J; Garcia‐vallvé S; Pujadas G; Ojeda‐montes MJ; Gimeno A; Arola L; Minghetti L; Beltrán‐debón R; Mulero M (2021). Anti‐inflammatory and immunomodulatory effects of the grifola frondosa natural compound o‐orsellinaldehyde on lps‐challenged murine primary glial cells. Roles of nf‐κβ and mapk. Pharmaceutics, 13(6), -. DOI: 10.3390/pharmaceutics13060806

Article's DOI: 10.3390/pharmaceutics13060806

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications