Author, as appears in the article.: Villar, Beatriz; Bertran, Laia; Aguilar, Carmen; Binetti, Jessica; Martinez, Salome; Sabench, Fatima; Real, Monica; Riesco, David; Paris, Marta; Del Castillo, Daniel; Richart, Cristobal; Auguet, Teresa;

Department: Medicina i Cirurgia

e-ISSN: 2218-1989

URV's Author/s: Aguilar Crespillo, Carmen Isabel / Auguet Quintillà, Maria Teresa / Bertran Ramos, Laia / Binetti, Jessica Angela / Del Castillo Déjardin, Daniel / Martínez González, María Salomé / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima

Keywords: Women Proneurotensin Pro-neurotensin Nonalcoholic steatohepatitis Nonalcoholic fatty liver disease Neurotensin Lipid metabolism Gastric bypass Fatty liver-disease

Abstract: Recent studies suggest a link between pro-neurotensin (pro-NT) and nonalcoholic fatty liver disease (NAFLD), but the published data are conflicting. Thus, we aimed to analyze pro-NT levels in women with morbid obesity (MO) and NAFLD to investigate if this molecule is involved in NAFLD and liver lipid metabolism. Plasma levels of pro-NT were determined in 56 subjects with MO and 18 with normal weight (NW). All patients with MO were subclassified according to their liver histology into the normal liver (NL, n = 20) and NAFLD (n = 36) groups. The NAFLD group had 17 subjects with simple steatosis (SS) and 19 with nonalcoholic steatohepatitis (NASH). We used a chemiluminescence sandwich immunoassay to quantify pro-NT in plasma and RT-qPCR to evaluate the hepatic mRNA levels of several lipid metabolism-related genes. We reported that pro-NT levels were significantly higher in MO with NAFLD than in MO without NAFLD. Additionally, pro-NT levels were higher in NASH patients than in NL. The hepatic expression of lipid metabolism-related genes was found to be altered in NAFLD, as previously reported. Additionally, although pro-NT levels correlated with LDL, there was no association with the main lipid metabolism-related genes. These findings suggest that pro-NT could be related to NAFLD progression.

Thematic Areas: Molecular biology Medicina ii Farmacia Endocrinology, diabetes and metabolism Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry & molecular biology Biochemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: danieldel.castillo@urv.cat mariasalome.martinez@urv.cat cristobalmanuel.richart@urv.cat mariateresa.auguet@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat fatima.sabench@urv.cat laia.bertran@urv.cat jessicaangela.binetti@estudiants.urv.cat

Author identifier: 0000-0003-0456-3102 0000-0001-7024-7824 0000-0001-6185-2889 0000-0003-0852-6739 0000-0003-0396-6428 0000-0002-4440-562X 0000-0002-4440-562X 0000-0002-9262-8756 0000-0001-9052-1368 0000-0001-9660-7752

Record's date: 2023-06-24

Journal volume: 11

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2218-1989/11/6/373

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Metabolites. 11 (6):

APA: Villar, Beatriz; Bertran, Laia; Aguilar, Carmen; Binetti, Jessica; Martinez, Salome; Sabench, Fatima; Real, Monica; Riesco, David; Paris, Marta; Del C (2021). Circulating Levels of Pro-Neurotensin and Its Relationship with Nonalcoholic Steatohepatitis and Hepatic Lipid Metabolism. Metabolites, 11(6), -. DOI: 10.3390/metabo11060373

Article's DOI: 10.3390/metabo11060373

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications