Author, as appears in the article.: Sanchez-Velazquez, Oscar Abel; Mulero, Miquel; Cuevas-Rodriguez, Edith Oliva; Mondor, Martin; Arcand, Yves; Hernandez-alvarez, Alan Javier;
Department: Bioquímica i Biotecnologia
URV's Author/s: Mulero Abellán, Miguel
Keywords: Physiological process Phenolic-compounds Oxidative stress Natural colorants Gastrointestinal digestion Fruit juices Complex processes Capacity Biological-activity Bioavailability Bioactive properties Bioaccessibility Assay Antioxidants Antioxidant capacity Anti-oxidant activities Anthocyanins Anthocyanin content Absorption
Abstract: Gastrointestinal digestion (GID) is a physiological process that transforms the stability, bioaccessibility and antioxidant activity (AOX) of polyphenols from blackberries (Rubus spp.). This study aimed to investigate the effect of the INFOGEST (R) GID protocol on the phenolic stability, bioaccessibility and AOX of Mexican wild (WB) and commercial (CB) blackberries. After GID, the total phenolic and anthocyanin contents in blackberries decreased by >= 68% and >= 74%, respectively. More than 40 phenolics were identified during GID; most of them degraded completely during digestion. GID had a negative effect on the AOX of both fruits (>50%), but WB showed the highest antioxidant activities, as assessed by the ORAC, DPPH, reducing power and beta-carotene bleaching methods. In Caco-2 cells, the cell-based antioxidant activity of digested blackberries (p < 0.05) decreased by 48% in WB and by 56% in CB. The capacity to inhibit intracellular ROS decreased by 50% in WB and by up to 86% in CB, after digestion. GID is a complex process that impacts on the bioactive properties of food nutrients, especially phenolics. In vitro and cellular AOX of WB polyphenols withstood the gastrointestinal environment better than CB phenolics. The in vitro assays results suggest that phenolics from underutilized WB have a higher bioaccessibility and antioxidant capacity than the polyphenols from the most frequently consumed CB. However, whether this corresponds to a better bioaccessibility in humans remains to be determined in future work.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biochemistry & molecular biology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: miquel.mulero@urv.cat
Record's date: 2023-02-19
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://pubs.rsc.org/en/content/articlelanding/2021/FO/D1FO00986A
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Food & Function. 12 (16): 7358-7378
APA: Sanchez-Velazquez, Oscar Abel; Mulero, Miquel; Cuevas-Rodriguez, Edith Oliva; Mondor, Martin; Arcand, Yves; Hernandez-alvarez, Alan Javier; (2021). In vitro gastrointestinal digestion impact on stability, bioaccessibility and antioxidant activity of polyphenols from wild and commercial blackberries (Rubus spp.). Food & Function, 12(16), 7358-7378. DOI: 10.1039/d1fo00986a
Article's DOI: 10.1039/d1fo00986a
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV