Articles producció científica> Ciències Mèdiques Bàsiques

MARTX Toxin in the Zoonotic Serovar of Vibrio vulnificus Triggers an Early Cytokine Storm in Mice

  • Identification data

    Identifier: imarina:9223940
    Authors:
    Murciano, CeliaLee, Chung-TeFernandez-Bravo, AnaHsieh, Tsung-HanFouz, BelenHor, Lien-IAmaro, Carmen
    Abstract:
    Vibrio vulnificus biotype 2-serovar E is a zoonotic clonal complex that can cause death by sepsis in humans and fish. Unlike other biotypes, Bt2 produces a unique type of MARTX(Vv) (Multifunctional-Autoprocessive-Repeats-in-Toxin; RtxA1(3)), which is encoded by a gene duplicated in the pVvBt2 plasmid and chromosome II. In this work, we analyzed the activity of this toxin and its role in human sepsis by performing in vitro, ex vivo, and in vivo assays. First, we demonstrated that the ACD domain, present exclusively in this toxin variant, effectively has an actin-cross-linking activity. Second, we determined that the whole toxin caused death of human endotheliocytes and monocytes by lysis and apoptosis, respectively. Finally, we tested the hypothesis that RtxA1(3) contributes to human death caused by this zoonotic serovar by triggering an early cytokine storm in blood. To this end, we used a Bt2-SerE strain (R99) together with its rtxA1(3) deficient mutant, and a Bt1 strain (YJ016) producing RtxA1(1) (the most studied MARTX(Vv)) together with its rtxA1(1) deficient mutant, as controls. Our results showed that RtxA1(3) was essential for virulence, as R99 Delta Delta rtxA1(3) was completely avirulent in our murine model of infection, and that R99, but not strain YJ016, induced an early, strong and dysregulated immune response involving the up-regulation of a high number of genes. This dysregulated immune response was directly linked to RtxA1(3). Based on these results and those obtained ex vivo (human blood), we propose a model of infection for the zoonotic serovar of V. vulnificus, in which RtxA1(3) would act as a sepsis-inducing toxin.
  • Others:

    Author, as appears in the article.: Murciano, Celia; Lee, Chung-Te; Fernandez-Bravo, Ana; Hsieh, Tsung-Han; Fouz, Belen; Hor, Lien-I; Amaro, Carmen;
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Fernández Bravo, Ana
    Keywords: Virulence plasmid Vibrio V. vulnificus Sepsis Rtx toxin Qpcr array Pathogen Metalloprotease Martx Infection Identification Gene expression Escherichia-coli Domain Biotype 2 Bacteria
    Abstract: Vibrio vulnificus biotype 2-serovar E is a zoonotic clonal complex that can cause death by sepsis in humans and fish. Unlike other biotypes, Bt2 produces a unique type of MARTX(Vv) (Multifunctional-Autoprocessive-Repeats-in-Toxin; RtxA1(3)), which is encoded by a gene duplicated in the pVvBt2 plasmid and chromosome II. In this work, we analyzed the activity of this toxin and its role in human sepsis by performing in vitro, ex vivo, and in vivo assays. First, we demonstrated that the ACD domain, present exclusively in this toxin variant, effectively has an actin-cross-linking activity. Second, we determined that the whole toxin caused death of human endotheliocytes and monocytes by lysis and apoptosis, respectively. Finally, we tested the hypothesis that RtxA1(3) contributes to human death caused by this zoonotic serovar by triggering an early cytokine storm in blood. To this end, we used a Bt2-SerE strain (R99) together with its rtxA1(3) deficient mutant, and a Bt1 strain (YJ016) producing RtxA1(1) (the most studied MARTX(Vv)) together with its rtxA1(1) deficient mutant, as controls. Our results showed that RtxA1(3) was essential for virulence, as R99 Delta Delta rtxA1(3) was completely avirulent in our murine model of infection, and that R99, but not strain YJ016, induced an early, strong and dysregulated immune response involving the up-regulation of a high number of genes. This dysregulated immune response was directly linked to RtxA1(3). Based on these results and those obtained ex vivo (human blood), we propose a model of infection for the zoonotic serovar of V. vulnificus, in which RtxA1(3) would act as a sepsis-inducing toxin.
    Thematic Areas: Saúde coletiva Química Odontología Microbiology (medical) Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Infectious diseases Immunology Geociências Farmacia Ensino Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: ana.fernandez@urv.cat
    Author identifier: 0000-0001-7637-5958
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.frontiersin.org/articles/10.3389/fcimb.2017.00332/full
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Frontiers In Cellular And Infection Microbiology. 7
    APA: Murciano, Celia; Lee, Chung-Te; Fernandez-Bravo, Ana; Hsieh, Tsung-Han; Fouz, Belen; Hor, Lien-I; Amaro, Carmen; (2017). MARTX Toxin in the Zoonotic Serovar of Vibrio vulnificus Triggers an Early Cytokine Storm in Mice. Frontiers In Cellular And Infection Microbiology, 7(), -. DOI: 10.3389/fcimb.2017.00332
    Article's DOI: 10.3389/fcimb.2017.00332
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    Publication Type: Journal Publications
  • Keywords:

    Immunology,Infectious Diseases,Medicine (Miscellaneous),Microbiology,Microbiology (Medical)
    Virulence plasmid
    Vibrio
    V. vulnificus
    Sepsis
    Rtx toxin
    Qpcr array
    Pathogen
    Metalloprotease
    Martx
    Infection
    Identification
    Gene expression
    Escherichia-coli
    Domain
    Biotype 2
    Bacteria
    Saúde coletiva
    Química
    Odontología
    Microbiology (medical)
    Microbiology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Infectious diseases
    Immunology
    Geociências
    Farmacia
    Ensino
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Biotecnología
    Biodiversidade
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