Articles producció científica> Enginyeria Química

Carbon Nano-Onion Peroxidase Composite Biosensor for Electrochemical Detection of 2,4-D and 2,4,5-T

  • Identification data

    Identifier: imarina:9225836
    Handle: https://hdl.handle.net/20.500.11797/imarina9225836
    Authors:
    Sok, VibolFragoso, Alex
    Abstract:
    Featured Application Electrochemical detection of phenoxy herbicides based on peroxidase inhibition using a carbon nano-onion modified biosensor. Carbon nano-onions are emerging electrode materials in biosensing due to their high conductivity and biocompatibility. Phenoxy-based herbicides are a source of environmental contamination that can be detected using their property to inhibit the activity of some enzymes. Here we report a biosensor based on peroxidase immobilized on carbon nano-onions in a cyclodextrin polymer matrix for the amperometric detection of 2,4-D and 2,4,5-T. The inhibition mechanism of 2,4-D and 2,4,5-T on peroxidase activity was first elucidated by activity measurements and molecular docking. The biosensor was characterized by electrochemical and microscopy methods and applied to the amperometric detection of these herbicides. The incorporation of carbon nano-onions enhanced the sensitivity of the biosensor and improved its stability and repeatability. The application of the developed biosensor to the detection of 2,4-D in soil and 2,4,5-T in river water samples is also reported.

  • Others:

    Author, as appears in the article.: Sok, Vibol; Fragoso, Alex
    Department: Enginyeria Química
    URV's Author/s: Fragoso Sierra, Alex / SOK, VIBOL
    Keywords: Phenoxy herbicide Pesticides Peroxidase Immunosensors Horseradish-peroxidase Glyphosate Electrochemical biosensor Electrochemical biosen-sor Cyclodextrin Carbon nano-onion 5-t 4-d 4 2,4-dichlorophenoxyacetic acid 2,4-d 2,4,5-t 2
    Abstract: Featured Application Electrochemical detection of phenoxy herbicides based on peroxidase inhibition using a carbon nano-onion modified biosensor. Carbon nano-onions are emerging electrode materials in biosensing due to their high conductivity and biocompatibility. Phenoxy-based herbicides are a source of environmental contamination that can be detected using their property to inhibit the activity of some enzymes. Here we report a biosensor based on peroxidase immobilized on carbon nano-onions in a cyclodextrin polymer matrix for the amperometric detection of 2,4-D and 2,4,5-T. The inhibition mechanism of 2,4-D and 2,4,5-T on peroxidase activity was first elucidated by activity measurements and molecular docking. The biosensor was characterized by electrochemical and microscopy methods and applied to the amperometric detection of these herbicides. The incorporation of carbon nano-onions enhanced the sensitivity of the biosensor and improved its stability and repeatability. The application of the developed biosensor to the detection of 2,4-D in soil and 2,4,5-T in river water samples is also reported.
    Thematic Areas: Química Process chemistry and technology Physics, applied Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) Materiais Instrumentation General materials science General engineering Fluid flow and transfer processes Engineering, multidisciplinary Engineering (miscellaneous) Engineering (all) Engenharias ii Engenharias i Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Chemistry, multidisciplinary Biodiversidade Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: alex.fragoso@urv.cat
    Author identifier: 0000-0003-4839-6094
    Record's date: 2024-10-12
    Journal volume: 11
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2076-3417/11/15/6889
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Applied Sciences-Basel. 11 (15): 6889-
    APA: Sok, Vibol; Fragoso, Alex (2021). Carbon Nano-Onion Peroxidase Composite Biosensor for Electrochemical Detection of 2,4-D and 2,4,5-T. Applied Sciences-Basel, 11(15), 6889-. DOI: 10.3390/app11156889
    Article's DOI: 10.3390/app11156889
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications

  • Keywords:

    Chemistry, Multidisciplinary,Computer Science Applications,Engineering (Miscellaneous),Engineering, Multidisciplinary,Fluid Flow and Transfer Processes,Instrumentation,Materials Science (Miscellaneous),Materials Science, Multidisciplinary,Physics, Applied,Process Chemistry and Technology
    Phenoxy herbicide
    Pesticides
    Peroxidase
    Immunosensors
    Horseradish-peroxidase
    Glyphosate
    Electrochemical biosensor
    Electrochemical biosen-sor
    Cyclodextrin
    Carbon nano-onion
    5-t
    4-d
    4
    2,4-dichlorophenoxyacetic acid
    2,4-d
    2,4,5-t
    2
    Química
    Process chemistry and technology
    Physics, applied
    Materials science, multidisciplinary
    Materials science (miscellaneous)
    Materials science (all)
    Materiais
    Instrumentation
    General materials science
    General engineering
    Fluid flow and transfer processes
    Engineering, multidisciplinary
    Engineering (miscellaneous)
    Engineering (all)
    Engenharias ii
    Engenharias i
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, multidisciplinary
    Biodiversidade
    Astronomia / física

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