Author, as appears in the article.: Suarez-Garcia, Susana; Caimari, Antoni; Bas, Josep M. del; Lalanza, Jaume; Escorihuela, Rosa M.; Suarez, Manuel; Torres-Fuentes, Cristina; Arola, Lluis;

Department: Bioquímica i Biotecnologia

URV's Author/s: Arola Ferrer, Luis Maria / SUÁREZ GARCÍA, SUSANA / Suárez Recio, Manuel / Torres Fuentes, Cristina

Keywords: Serum Resistance training Profile Prevalence Plasma Performance liquid-chromatography Obesity Metabolic syndrome Men Markers Lysophosphatidylcholine Lysoglycerophospholipids Cafeteria diet

Abstract: The daily practice of physical exercise and a balanced diet are recommended to prevent metabolic syndrome (MetS). As MetS is a multifactorial disorder associated with the development of serious diseases, the advancement of comprehensive biomarkers could aid in an accurate diagnosis. In this regard, it is known that gut microbiota is altered in MetS, and especially, lipid metabolites species are highly modified, thus emerging as potential biomarkers. In preliminary studies, we observed that alterations in serum lysoglycerophospholipids (Lyso-PLs) were shared between animals with diet-induced MetS and those performing resistance exercises assiduously. Therefore, our objective was the targeted determination of the lysophospholipidome in young rats fed a standard (ST) or a cafeteria diet (CAF) and submitted to different training intensities to evaluate its potential as a biomarker of a detrimental lifestyle. Targeted metabolomics focused on lysophosphatidylcholines (Lyso-PCs) and lysophosphatidylethanolamines (Lyso-PEs) and multivariate statistics were used to achieve an integral understanding. Chronic intake of CAF altered the serological levels of both lipid subclasses. Twenty-two Lyso-PLs were significantly altered by CAF, from which we selected Lyso-PCs (14:0), (17:1) and (20:2) and Lyso-PEs (18:2) and (18:3) as they were enough to achieve an optimal prediction. The main effect of physical training was decreased Lyso-PEs levels with disparities among training intensities for each diet. We concluded that an examination of the lysophospholipidome reveals the general state of the metabolome in young female rats, especially due to intake of an MetS-inducing diet, thus highlighting the importance of this family of compounds in lipid disorders.

Thematic Areas: Molecular biology Medicina ii Farmacia Endocrinology, diabetes and metabolism Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry & molecular biology Biochemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: cristina.torres@urv.cat lluis.arola@urv.cat manuel.suarez@urv.cat

Author identifier: 0000-0002-2917-6910 0000-0003-2767-1974 0000-0003-0122-8253

Record's date: 2023-02-23

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2218-1989/11/8/471

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Metabolites. 11 (8):

APA: Suarez-Garcia, Susana; Caimari, Antoni; Bas, Josep M. del; Lalanza, Jaume; Escorihuela, Rosa M.; Suarez, Manuel; Torres-Fuentes, Cristina; Arola, Llui (2021). Chronic Effect of a Cafeteria Diet and Intensity of Resistance Training on the Circulating Lysophospholipidome in Young Rats. Metabolites, 11(8), -. DOI: 10.3390/metabo11080471

Article's DOI: 10.3390/metabo11080471

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications