Author, as appears in the article.: Valente, Sabrina; Ciavarella, Carmen; Hernandez-Aguilera, Anna; Salvador, Fernandez-Arroyo; Buzzi, Marina; Joven, Jorge; Pasquinelli, Gianandrea;
Department: Medicina i Cirurgia
URV's Author/s: Joven Maried, Jorge
Keywords: Stromal cells Stem cell metabolomics Spheroids Spheres Pathways Human mesenchymal stem cells Culture Arteries
Abstract: The ability to form spheroids under non-adherent conditions is a well-known property of human mesenchymal stem cells (hMSCs), in addition to stemness and multilineage differentiation features. In the present study, we tested the ability of hMSCs isolated from the vascular wall (hVW-MSCs) to grow as spheres, and provide a characterization of this 3D model. hVW-MSCs were isolated from femoral arteries through enzymatic digestion. Spheres were obtained using ultra-low attachment and hanging drop methods. Immunophenotype and pluripotent genes (SOX-2, OCT-4, NANOG) were analyzed by immunocytochemistry and real-time PCR, respectively. Spheres histological and ultrastructural architecture were examined. Cell viability and proliferative capacity were measured using LIVE/DEATH assay and ki-67 proliferation marker. Metabolomic profile was obtained with liquid chromatography-mass spectrometry. In 2D, hVW-MSCs were spindle-shaped, expressed mesenchymal antigens, and displayed mesengenic potential. 3D cultures of hVW-MSCs were CD44(+), CD105(low), CD90(low), exhibited a low propensity to enter the cell cycle as indicated by low percentage of ki-67 expression and accumulated intermediate metabolites pointing to slowed metabolism. The 3D model of hVW-MSCs exhibits stemness, dormancy and slow metabolism, typically observed in stem cell niches. This culture strategy can represent an accurate model to investigate hMSCs features for future clinical applications in the vascular field.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Microscopy Medicina veterinaria Medicina iii Medicina ii Medicina i Medical laboratory technology Materiais Matemática / probabilidade e estatística Interdisciplinar Instrumentation Histology Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biology Biodiversidade Astronomia / física Anatomy & morphology Anatomy
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: jorge.joven@urv.cat
Author identifier: 0000-0003-2749-4541
Record's date: 2024-08-31
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jemt.23918
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Microscopy Research And Technique. 85 (2): 447-459
APA: Valente, Sabrina; Ciavarella, Carmen; Hernandez-Aguilera, Anna; Salvador, Fernandez-Arroyo; Buzzi, Marina; Joven, Jorge; Pasquinelli, Gianandrea; (2022). Phenotypic, morphological, and metabolic characterization of vascular-spheres from human vascular mesenchymal stem cells. Microscopy Research And Technique, 85(2), 447-459. DOI: 10.1002/jemt.23918
Article's DOI: 10.1002/jemt.23918
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV