Articles producció científica> Bioquímica i Biotecnologia

High-fat diet alters stress behavior, inflammatory parameters and gut microbiota in Tg APP mice in a sex-specific manner

  • Identification data

    Identifier: imarina:9229557
  • Authors:

    Yanguas-Casas, Natalia
    Torres, Cristina
    Crespo-Castrillo, Andrea
    Diaz-Pacheco, Sonia
    Healy, Kiera
    Stanton, Catherine
    Chowen, Julie A.
    Garcia-Segura, Luis M.
    Angeles Arevalo, Maria
    Cryan, John F.
    de Ceballos, Maria L.
  • Others:

    Author, as appears in the article.: Yanguas-Casas, Natalia; Torres, Cristina; Crespo-Castrillo, Andrea; Diaz-Pacheco, Sonia; Healy, Kiera; Stanton, Catherine; Chowen, Julie A.; Garcia-Segura, Luis M.; Angeles Arevalo, Maria; Cryan, John F.; de Ceballos, Maria L.;
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Torres Fuentes, Cristina
    Keywords: Transgenic mouse model Tgapp mice Sex differences Risk Phagocytosis Obesity Microglia Microbiota Memory deficits Inflammation Driving-force Diet Cytokines Cognitive impairment Brain Anxiety Amyloid pathology Alzheimers-disease Alzheimer's disease Akkermansia-muciniphila
    Abstract: Long-term high-fat diet (HFD) consumption commonly leads to obesity, a major health concern of western societies and a risk factor for Alzheimer's disease (AD). Both conditions present glial activation and inflammation and show sex differences in their incidence, clinical manifestation, and disease course. HFD intake has an important impact on gut microbiota, the bacteria present in the gut, and microbiota dysbiosis is associated with inflammation and certain mental disorders such as anxiety. In this study, we have analyzed the effects of a prolonged (18 weeks, starting at 7 months of age) HFD on male and female mice, both wild type (WT) and TgAPP mice, a model for AD, investigating the behavioral profile, gut microbiota composition and inflammatory/phagocytosis-related gene expression in hippocampus. In the open-field test, no overt differences in motor activity were observed between male and female or WT and TgAPP mice on a low-fat diet (LFD). However, HFD induced anxiety, as judged by decreased motor activity and increased time in the margins in the open-field, and a trend towards increased immobility time in the tail suspension test, with increased defecation. Intriguingly, female TgAPP mice on HFD showed less immobility and defecation compared to female WT mice on HFD. HFD induced dysbiosis of gut microbiota, resulting in reduced microbiota diversity and abundance compared with LFD fed mice, with some significant differences due to sex and little effect of genotype. Gene expression of proinflammatory/phagocytic markers in the hippocampus were not different between male and female WT mice, and in TgAPP mice of both sexes, some cytokines (IL-6 and IFN gamma) were higher than in WT mice on LFD, more so in female TgAPP (IL-6). HFD induced few alterations in mRNA expression of inflammatory/phagocytosis-related genes in male mice, whether WT (IL-1 beta, MHCII), or TgAPP (IL-6). However, in female TgAPP, altered gene expression returned towards control levels following prolonged HFD (IL-6, IL-12 beta, TNF alpha, CD36, IRAK4, PYRY6). In summary, we demonstrate that HFD induces anxiogenic symptoms, marked alterations in gut microbiota, and increased expression of inflammatory genes, except for female TgAPP that appear to be resistant to the diet effects. Lifestyle interventions should be introduced to prevent AD onset or exacerbation by reducing inflammation and its associated symptoms; however, our results suggest that the eventual goal of developing prevention and treatment strategies should take sex into consideration.
    Thematic Areas: Odontología Neurosciences Neurology Medicina ii Medicina i Interdisciplinar General medicine Engenharias ii Educação física Clinical neurology Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: cristina.torres@urv.cat
    Author identifier: 0000-0002-2917-6910
    Record's date: 2024-07-20
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/pii/S0969996121002448?via%3Dihub
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Neurobiology Of Disease. 159
    APA: Yanguas-Casas, Natalia; Torres, Cristina; Crespo-Castrillo, Andrea; Diaz-Pacheco, Sonia; Healy, Kiera; Stanton, Catherine; Chowen, Julie A.; Garcia-Se (2021). High-fat diet alters stress behavior, inflammatory parameters and gut microbiota in Tg APP mice in a sex-specific manner. Neurobiology Of Disease, 159(), -. DOI: 10.1016/j.nbd.2021.105495
    Article's DOI: 10.1016/j.nbd.2021.105495
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Clinical Neurology,Neurology,Neurosciences
    Transgenic mouse model
    Tgapp mice
    Sex differences
    Risk
    Phagocytosis
    Obesity
    Microglia
    Microbiota
    Memory deficits
    Inflammation
    Driving-force
    Diet
    Cytokines
    Cognitive impairment
    Brain
    Anxiety
    Amyloid pathology
    Alzheimers-disease
    Alzheimer's disease
    Akkermansia-muciniphila
    Odontología
    Neurosciences
    Neurology
    Medicina ii
    Medicina i
    Interdisciplinar
    General medicine
    Engenharias ii
    Educação física
    Clinical neurology
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Biotecnología
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