Articles producció científica> Medicina i Cirurgia

Impaired mRNA splicing and proteostasis in preadipocytes in obesity related metabolic disease

  • Identification data

    Identifier: imarina:9230389
    Authors:
    Sánchez-Ceinos JGuzmán-Ruiz RRangel-Zúñiga OALópez-Alcalá JMoreno-Caño EDel Río-Moreno MRomero-Cabrera JLPérez-Martínez PMaymo-Masip EVendrell JFernández-Veledo SFernández-Real JMLaurencikiene JRydén MMembrives ALuque RMLópez-Miranda JMalagón MM
    Abstract:
    Preadipocytes are crucial for healthy adipose tissue expansion. Preadipocyte differentiation is altered in obese individuals, which has been proposed to contribute to obesity-associated metabolic disturbances. Here, we aimed at identifying the pathogenic processes underlying impaired adipocyte differentiation in obese individuals with insulin resistance (IR)/type 2 diabetes (T2D). We report that down-regulation of a key member of the major spliceosome, PRFP8/PRP8, as observed in IR/T2D preadipocytes from subcutaneous (SC) fat, prevented adipogenesis by altering both the expression and splicing patterns of adipogenic transcription factors and lipid droplet-related proteins, while adipocyte differentiation was restored upon recovery of PRFP8/PRP8 normal levels. Adipocyte differentiation was also compromised under conditions of endoplasmic reticulum (ER)-associated protein degradation (ERAD) hyperactivation, as occurs in SC and omental (OM) preadipocytes in IR/T2D obesity. Thus, targeting mRNA splicing and ER proteostasis in preadipocytes could improve adipose tissue function and thus contribute to metabolic health in obese individuals.
  • Others:

    Author, as appears in the article.: Sánchez-Ceinos J; Guzmán-Ruiz R; Rangel-Zúñiga OA; López-Alcalá J; Moreno-Caño E; Del Río-Moreno M; Romero-Cabrera JL; Pérez-Martínez P; Maymo-Masip E; Vendrell J; Fernández-Veledo S; Fernández-Real JM; Laurencikiene J; Rydén M; Membrives A; Luque RM; López-Miranda J; Malagón MM
    Department: Medicina i Cirurgia Bioquímica i Biotecnologia
    URV's Author/s: Fernandez Veledo, Sonia / Maymo Masip, Elsa / Vendrell Ortega, Juan José
    Keywords: Preadipocytes Human adipose-tissue upr tool stress rna splicing obesity-related metabolic diseases insulin-resistance human adipocytes human homeostasis fat erad er-proteostasis differentiation cells adipogenesis
    Abstract: Preadipocytes are crucial for healthy adipose tissue expansion. Preadipocyte differentiation is altered in obese individuals, which has been proposed to contribute to obesity-associated metabolic disturbances. Here, we aimed at identifying the pathogenic processes underlying impaired adipocyte differentiation in obese individuals with insulin resistance (IR)/type 2 diabetes (T2D). We report that down-regulation of a key member of the major spliceosome, PRFP8/PRP8, as observed in IR/T2D preadipocytes from subcutaneous (SC) fat, prevented adipogenesis by altering both the expression and splicing patterns of adipogenic transcription factors and lipid droplet-related proteins, while adipocyte differentiation was restored upon recovery of PRFP8/PRP8 normal levels. Adipocyte differentiation was also compromised under conditions of endoplasmic reticulum (ER)-associated protein degradation (ERAD) hyperactivation, as occurs in SC and omental (OM) preadipocytes in IR/T2D obesity. Thus, targeting mRNA splicing and ER proteostasis in preadipocytes could improve adipose tissue function and thus contribute to metabolic health in obese individuals.
    Thematic Areas: Saúde coletiva Química Nutrição Neuroscience (miscellaneous) Neuroscience (all) Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Interdisciplinar Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Geografía Geociências General neuroscience General medicine General immunology and microbiology General biochemistry,genetics and molecular biology Farmacia Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biology Biodiversidade Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: sonia.fernandez@urv.cat elsa.maymo@urv.cat juanjose.vendrell@urv.cat
    Author identifier: 0000-0003-2906-3788 0000-0002-9133-3120 0000-0002-6994-6115
    Record's date: 2024-07-27
    Papper version: info:eu-repo/semantics/publishedVersion
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Elife. 10
    APA: Sánchez-Ceinos J; Guzmán-Ruiz R; Rangel-Zúñiga OA; López-Alcalá J; Moreno-Caño E; Del Río-Moreno M; Romero-Cabrera JL; Pérez-Martínez P; Maymo-Masip E (2021). Impaired mRNA splicing and proteostasis in preadipocytes in obesity related metabolic disease. Elife, 10(), -. DOI: 10.7554/eLife.65996
    Article's DOI: 10.7554/eLife.65996
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry, Genetics and Molecular Biology (Miscellaneous),Biology,Immunology and Microbiology (Miscellaneous),Medicine (Miscellaneous),Neuroscience (Miscellaneous)
    Preadipocytes
    Human adipose-tissue
    upr
    tool
    stress
    rna splicing
    obesity-related metabolic diseases
    insulin-resistance
    human adipocytes
    human
    homeostasis
    fat
    erad
    er-proteostasis
    differentiation
    cells
    adipogenesis
    Saúde coletiva
    Química
    Nutrição
    Neuroscience (miscellaneous)
    Neuroscience (all)
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina ii
    Medicina i
    Interdisciplinar
    Immunology and microbiology (miscellaneous)
    Immunology and microbiology (all)
    Geografía
    Geociências
    General neuroscience
    General medicine
    General immunology and microbiology
    General biochemistry,genetics and molecular biology
    Farmacia
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Biotecnología
    Biology
    Biodiversidade
    Biochemistry, genetics and molecular biology (miscellaneous)
    Biochemistry, genetics and molecular biology (all)
    Astronomia / física
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