Articles producció científica> Química Analítica i Química Orgànica

Comparison of different chiral selectors for the enantiomeric determination of amphetamine-type substances in human urine by solid-phase extraction followed by capillary electrophoresis-tandem mass spectrometry

  • Identification data

    Identifier: imarina:9230615
    Authors:
    Pascual-Caro, SergiBorrull, FrancescAguilar, CarmeCalull, Marta
    Abstract:
    The present study develops a method for the enantioseparation of a group of amphetamines and their metabolites in urine by CE coupled to MS/MS (CE-MS/MS). Amphetamines present a chiral center and thus two enantiomers, which is important from a toxicological point of view because they may have different pharmacokinetic and pharmacological properties. It is therefore essential to find suitable methods to distinguish both enantiomers. Today the use of CE is becoming more important in this field since, with the simple addition of a chiral selector to the background electrolyte, the enantioseparation can easily be achieved. However, when CE is coupled to MS, the use of volatile chiral selectors and compatible background electrolytes or other strategies such as the countercurrent migration approach are required to avoid contamination of the ion source from nonvolatile species. In the present study, we use the latter strategy to evaluate six different chiral selectors using CE-MS/MS. As a sample pre-treatment, two cationic-exchange sorbents-Oasis WCX and Oasis MCX-are compared for the urine pre-treatment. Using this method, it was possible to achieve the complete chiral separation of the amphetamines under study with detection limits ranging between 0.8 and 1.5 ng/mL and method quantification limits between 2.0 and 8.0 ng/mL. Matrix-matched calibration curves up to 150 ng/mL were used to cover the usual concentration ranges at which amphetamines have generally been found in toxicological and forensic analyses.
  • Others:

    Author, as appears in the article.: Pascual-Caro, Sergi; Borrull, Francesc; Aguilar, Carme; Calull, Marta;
    Department: Química Analítica i Química Orgànica
    URV's Author/s: Aguilar Anguera, Maria del Carmen / Borrull Ballarín, Francesc / Calull Blanch, Marta / Pascual Caro, Sergi
    Keywords: Stimulants Separation Ms Methamphetamine Metabolites Lisdexamfetamine Liquid-chromatography Human urine Hair Derivatization Derivatives Chiral selectors Ce-ms/ms Ce-ms Cation-exchange sorbents Amphetamine-type substances
    Abstract: The present study develops a method for the enantioseparation of a group of amphetamines and their metabolites in urine by CE coupled to MS/MS (CE-MS/MS). Amphetamines present a chiral center and thus two enantiomers, which is important from a toxicological point of view because they may have different pharmacokinetic and pharmacological properties. It is therefore essential to find suitable methods to distinguish both enantiomers. Today the use of CE is becoming more important in this field since, with the simple addition of a chiral selector to the background electrolyte, the enantioseparation can easily be achieved. However, when CE is coupled to MS, the use of volatile chiral selectors and compatible background electrolytes or other strategies such as the countercurrent migration approach are required to avoid contamination of the ion source from nonvolatile species. In the present study, we use the latter strategy to evaluate six different chiral selectors using CE-MS/MS. As a sample pre-treatment, two cationic-exchange sorbents-Oasis WCX and Oasis MCX-are compared for the urine pre-treatment. Using this method, it was possible to achieve the complete chiral separation of the amphetamines under study with detection limits ranging between 0.8 and 1.5 ng/mL and method quantification limits between 2.0 and 8.0 ng/mL. Matrix-matched calibration curves up to 150 ng/mL were used to cover the usual concentration ranges at which amphetamines have generally been found in toxicological and forensic analyses.
    Thematic Areas: Zootecnia / recursos pesqueiros Química Medicina ii Medicina i Materiais Interdisciplinar General medicine Farmacia Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Chemistry, analytical Biotecnología Biodiversidade Biochemistry Biochemical research methods Analytical chemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: sergi.pascual@estudiants.urv.cat carme.aguilar@urv.cat marta.calull@urv.cat francesc.borrull@urv.cat
    Author identifier: 0000-0001-8890-5100 0000-0002-5913-2599 0000-0002-5258-1949 0000-0003-2718-9336
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/submittedVersion
    Link to the original source: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/elps.202100231
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Electrophoresis. 43 (3): 437-445
    APA: Pascual-Caro, Sergi; Borrull, Francesc; Aguilar, Carme; Calull, Marta; (2022). Comparison of different chiral selectors for the enantiomeric determination of amphetamine-type substances in human urine by solid-phase extraction followed by capillary electrophoresis-tandem mass spectrometry. Electrophoresis, 43(3), 437-445. DOI: 10.1002/elps.202100231
    Article's DOI: 10.1002/elps.202100231
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2022
    Publication Type: Journal Publications
  • Keywords:

    Analytical Chemistry,Biochemical Research Methods,Biochemistry,Chemistry, Analytical,Clinical Biochemistry
    Stimulants
    Separation
    Ms
    Methamphetamine
    Metabolites
    Lisdexamfetamine
    Liquid-chromatography
    Human urine
    Hair
    Derivatization
    Derivatives
    Chiral selectors
    Ce-ms/ms
    Ce-ms
    Cation-exchange sorbents
    Amphetamine-type substances
    Zootecnia / recursos pesqueiros
    Química
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    General medicine
    Farmacia
    Clinical biochemistry
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Biochemistry
    Biochemical research methods
    Analytical chemistry
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