Author, as appears in the article.: Shkembi, Xhensila; Skouridou, Vasso; Svobodova, Marketa; Leonardo, Sandra; Bashammakh, Abdulaziz S; Alyoubi, Abdulrahman O; Campas, Monica; O'Sullivan, Ciara K
Department: Enginyeria Química
URV's Author/s: CAMPAS HOMS, MONICA / O'SULLIVAN, CIARA KATHLEEN / Shkembi, Xhensila / Skouridou, Vasoula / SVOBODOVÁ, MARKÉTA
Keywords: Toxic materials Tetrodotoxin Tetraodontiformes Surface-plasmon resonance Reliable detection Pufferfish Prevention strategies Mass spectrometry Marine toxins Marine species Liquid chromatography - mass spectrometries Liquid chromatography Immunology Immunoassay Health safety Health risks Fast detections Cost effectiveness Chromatography, liquid Chemical detection Binding energy Aptamers Aptamer assay Antibodies Animals ttx toxicity selection lagocephalus-sceleratus immunoassay fish dna-aptamers capture-selex 1st detection
Abstract: The marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due to its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated marine species like pufferfish due to its expansion to nonendemic areas has increased the need for fast and reliable detection of the toxin to effectively implement prevention strategies. Liquid chromatography-mass spectrometry is considered the most accurate method, although competitive immunoassays have also been reported. In this work, we sought to develop an aptamer-based assay for the rapid, sensitive, and cost-effective detection of TTX in pufferfish. Using capture-SELEX combined with next-generation sequencing, aptamers were identified, and their binding properties were evaluated. Finally, a highly sensitive and user-friendly hybrid antibody-aptamer sandwich assay was developed with superior performance compared to several assays reported in the literature and commercial immunoassay kits. The assay was successfully applied to the quantification of TTX in pufferfish extracts, and the results obtained correlated very well with a competitive magnetic bead-based immunoassay performed in parallel for comparison. This is one of the very few works reported in the literature of such hybrid assays for small-molecule analytes whose compatibility with field samples is also demonstrated.
Thematic Areas: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: vasoula.skouridou@urv.cat monica.campas@urv.cat xhensila.shkembi@estudiants.urv.cat
Author identifier: 0000-0002-9712-5429 0000-0002-1220-7100
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://pubs.acs.org/doi/10.1021/acs.analchem.1c03671
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Analytical Chemistry. 93 (44): 14810-14819
APA: Shkembi, Xhensila; Skouridou, Vasso; Svobodova, Marketa; Leonardo, Sandra; Bashammakh, Abdulaziz S; Alyoubi, Abdulrahman O; Campas, Monica; O'Sullivan (2021). Hybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfish. Analytical Chemistry, 93(44), 14810-14819. DOI: 10.1021/acs.analchem.1c03671
Article's DOI: 10.1021/acs.analchem.1c03671
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications