Author, as appears in the article.: Rodríguez M; Rehues P; Iranzo V; Mora J; Balsells C; Guardiola M; Ribalta J
Department: Medicina i Cirurgia
URV's Author/s: Guardiola Guionnet, Montserrat / Rehues Masip, Pere / Ribalta Vives, Josep
Keywords: Type 2 diabetes Lipoproteins Cardiovascular disease Apolipoprotein c-iii type 2 diabetes triglyceride-rich lipoproteins protection of-function mutations metabolism low-density-lipoprotein insulin-resistance expression ciii cardiovascular disease beta-cell apolipoprotein c-iii
Abstract: Glycosylation of ApoC-III modulates its function in TG metabolism, with some variants being associated with a more atherogenic lipid profile. These associations have been studied in whole plasma but rarely in individual lipoprotein fractions. In this study, we aimed to measure the relative content of ApoC-III glycoforms in each lipoprotein fraction as a potential biomarker for TG metabolism and cardiovascular risk. Lipoprotein fractions were separated by differential ultracentrifugation of plasma samples from healthy subjects. Relative concentrations of seven ApoC-III variants were measured by MSIA. ApoC-III1, ApoC-III0b and ApoC-III2 were the most abundant glycoforms. There was high interindividual variability in the distribution of glycoforms across the study population but a uniform proportion in all lipoprotein fractions of each given subject. Two ApoC-III variants, ApoC-III0b and ApoC-III1d, negatively correlated with plasma and VLDL triglycerides irrespectively of VLDL size and were associated with increased LDL size when transported in LDL particles. ApoC-III0b also showed a negative correlation with lipoprotein-insulin resistance score. We have been able to measure seven ApoC-III glycoforms in each lipoprotein fraction, setting the basis for future studies exploring their role on cardiovascular risk. Some glycoforms suggest a less proatherogenic role on TG and lipoprotein metabolism. Significance: Apo CIII has an important role on plasma TG metabolism through different mechanisms and it is also involved in type 1 and type 2 Diabetes Mellitus. Different glycosylated forms of Apo CIII exist and they show different roles. For this reason, this protein has gained interest in the las years and the relationship between ApoC-III glycoforms and lipids, lipoproteins and metabolic disorders has been increasingly studied in the last years. Apo CIII glycoforms have been previously analysed in plasma, and the function of the main four glycoforms has been assessed in a variety of cohorts; but in the present study, ApoC-III glycoforms are measured in each lipoprotein fraction, which may be of clinical interest.
Thematic Areas: Zootecnia / recursos pesqueiros Química Psicología Odontología Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Farmacia Ensino Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biophysics Biodiversidade Biochemistry Biochemical research methods Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: pere.rehues@urv.cat pere.rehues@urv.cat montse.guardiola@urv.cat josep.ribalta@urv.cat
Author identifier: 0000-0002-9696-7384 0000-0002-8879-4719
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.sciencedirect.com/science/article/pii/S1874391921002979?via%3Dihub
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Journal Of Proteomics. 251 104398-
APA: Rodríguez M; Rehues P; Iranzo V; Mora J; Balsells C; Guardiola M; Ribalta J (2022). Distribution of seven ApoC-III glycoforms in plasma, VLDL, IDL, LDL and HDL of healthy subjects. Journal Of Proteomics, 251(), 104398-. DOI: 10.1016/j.jprot.2021.104398
Article's DOI: 10.1016/j.jprot.2021.104398
Entity: Universitat Rovira i Virgili
Journal publication year: 2022
Publication Type: Journal Publications
Repositori URV