Author, as appears in the article.: Sunyer-Figueres, Merce; Mas, Albert; Beltran, Gemma; Torija, Maria-Jesus
Department: Bioquímica i Biotecnologia
URV's Author/s: Beltran Casellas, Gemma / Mas Baron, Alberto / Sunyer Figueres, Mercè / Torija Martínez, María Jesús
Keywords: Yeast antioxidant response Superoxide dismutase Ros accumulation Ros accumula-tion Melatonin supplementation L-tryptophan metabolites Ethanol stress Catalase activity yeast-cells yeast antioxidant response tolerance superoxide dismutase stationary-phase ros accumulation oxidative stress membrane melatonin supplementation growth expression catalase activity alcoholic fermentation adaptive response
Abstract: During alcoholic fermentation, Saccharomyces cerevisiae is subjected to several stresses, among which ethanol is of capital importance. Melatonin, a bioactive molecule synthesized by yeast during alcoholic fermentation, has an antioxidant role and is proposed to contribute to counteract-ing fermentation-associated stresses. The aim of this study was to unravel the protective effect of melatonin on yeast cells subjected to ethanol stress. For that purpose, the effect of ethanol concentrations (6 to 12%) on a wine strain and a lab strain of S. cerevisiae was evaluated, monitoring the viability, growth capacity, mortality, and several indicators of oxidative stress over time, such as reactive oxygen species (ROS) accumulation, lipid peroxidation, and the activity of catalase and superoxide dismutase enzymes. In general, ethanol exposure reduced the cell growth of S. cerevisiae and increased mortality, ROS accumulation, lipid peroxidation and antioxidant enzyme activity. Melatonin supplementation softened the effect of ethanol, enhancing cell growth and decreasing oxidative damage by lowering ROS accumulation, lipid peroxidation, and antioxidant enzyme ac-tivities. However, the effects of melatonin were dependent on strain, melatonin concentration, and growth phase. The results of this study indicate that melatonin has a protective role against mild ethanol stress, mainly by reducing the oxidative stress triggered by this alcohol.
Thematic Areas: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: gemma.beltran@urv.cat merce.sunyer@estudiants.urv.cat merce.sunyer@estudiants.urv.cat mjesus.torija@urv.cat albert.mas@urv.cat
Author identifier: 0000-0002-7071-205X 0000-0001-6419-0745 0000-0002-0763-1679
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2076-3921/10/11/1735
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Antioxidants. 10 (11): 1735-
APA: Sunyer-Figueres, Merce; Mas, Albert; Beltran, Gemma; Torija, Maria-Jesus (2021). Protective effects of melatonin on saccharomyces cerevisiae under ethanol stress. Antioxidants, 10(11), 1735-. DOI: 10.3390/antiox10111735
Article's DOI: 10.3390/antiox10111735
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications