Articles producció científica> Bioquímica i Biotecnologia

Protective effects of melatonin on saccharomyces cerevisiae under ethanol stress

  • Identification data

    Identifier: imarina:9231334
    Authors:
    Sunyer-Figueres, MerceMas, AlbertBeltran, GemmaTorija, Maria-Jesus
    Abstract:
    During alcoholic fermentation, Saccharomyces cerevisiae is subjected to several stresses, among which ethanol is of capital importance. Melatonin, a bioactive molecule synthesized by yeast during alcoholic fermentation, has an antioxidant role and is proposed to contribute to counteract-ing fermentation-associated stresses. The aim of this study was to unravel the protective effect of melatonin on yeast cells subjected to ethanol stress. For that purpose, the effect of ethanol concentrations (6 to 12%) on a wine strain and a lab strain of S. cerevisiae was evaluated, monitoring the viability, growth capacity, mortality, and several indicators of oxidative stress over time, such as reactive oxygen species (ROS) accumulation, lipid peroxidation, and the activity of catalase and superoxide dismutase enzymes. In general, ethanol exposure reduced the cell growth of S. cerevisiae and increased mortality, ROS accumulation, lipid peroxidation and antioxidant enzyme activity. Melatonin supplementation softened the effect of ethanol, enhancing cell growth and decreasing oxidative damage by lowering ROS accumulation, lipid peroxidation, and antioxidant enzyme ac-tivities. However, the effects of melatonin were dependent on strain, melatonin concentration, and growth phase. The results of this study indicate that melatonin has a protective role against mild ethanol stress, mainly by reducing the oxidative stress triggered by this alcohol.
  • Others:

    Author, as appears in the article.: Sunyer-Figueres, Merce; Mas, Albert; Beltran, Gemma; Torija, Maria-Jesus
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Beltran Casellas, Gemma / Mas Baron, Alberto / Sunyer Figueres, Mercè / Torija Martínez, María Jesús
    Keywords: Yeast antioxidant response Superoxide dismutase Ros accumulation Ros accumula-tion Melatonin supplementation L-tryptophan metabolites Ethanol stress Catalase activity yeast-cells yeast antioxidant response tolerance superoxide dismutase stationary-phase ros accumulation oxidative stress membrane melatonin supplementation growth expression catalase activity alcoholic fermentation adaptive response
    Abstract: During alcoholic fermentation, Saccharomyces cerevisiae is subjected to several stresses, among which ethanol is of capital importance. Melatonin, a bioactive molecule synthesized by yeast during alcoholic fermentation, has an antioxidant role and is proposed to contribute to counteract-ing fermentation-associated stresses. The aim of this study was to unravel the protective effect of melatonin on yeast cells subjected to ethanol stress. For that purpose, the effect of ethanol concentrations (6 to 12%) on a wine strain and a lab strain of S. cerevisiae was evaluated, monitoring the viability, growth capacity, mortality, and several indicators of oxidative stress over time, such as reactive oxygen species (ROS) accumulation, lipid peroxidation, and the activity of catalase and superoxide dismutase enzymes. In general, ethanol exposure reduced the cell growth of S. cerevisiae and increased mortality, ROS accumulation, lipid peroxidation and antioxidant enzyme activity. Melatonin supplementation softened the effect of ethanol, enhancing cell growth and decreasing oxidative damage by lowering ROS accumulation, lipid peroxidation, and antioxidant enzyme ac-tivities. However, the effects of melatonin were dependent on strain, melatonin concentration, and growth phase. The results of this study indicate that melatonin has a protective role against mild ethanol stress, mainly by reducing the oxidative stress triggered by this alcohol.
    Thematic Areas: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: gemma.beltran@urv.cat merce.sunyer@estudiants.urv.cat merce.sunyer@estudiants.urv.cat mjesus.torija@urv.cat albert.mas@urv.cat
    Author identifier: 0000-0002-7071-205X 0000-0001-6419-0745 0000-0002-0763-1679
    Record's date: 2024-10-12
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2076-3921/10/11/1735
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Antioxidants. 10 (11): 1735-
    APA: Sunyer-Figueres, Merce; Mas, Albert; Beltran, Gemma; Torija, Maria-Jesus (2021). Protective effects of melatonin on saccharomyces cerevisiae under ethanol stress. Antioxidants, 10(11), 1735-. DOI: 10.3390/antiox10111735
    Article's DOI: 10.3390/antiox10111735
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry,Biochemistry & Molecular Biology,Cell Biology,Chemistry, Medicinal,Clinical Biochemistry,Food Science,Food Science & Technology,Molecular Biology,Physiology
    Yeast antioxidant response
    Superoxide dismutase
    Ros accumulation
    Ros accumula-tion
    Melatonin supplementation
    L-tryptophan metabolites
    Ethanol stress
    Catalase activity
    yeast-cells
    yeast antioxidant response
    tolerance
    superoxide dismutase
    stationary-phase
    ros accumulation
    oxidative stress
    membrane
    melatonin supplementation
    growth
    expression
    catalase activity
    alcoholic fermentation
    adaptive response
    Química
    Physiology
    Molecular biology
    Medicina ii
    Medicina i
    Interdisciplinar
    Food science & technology
    Food science
    Farmacia
    Engenharias ii
    Clinical biochemistry
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, medicinal
    Cell biology
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Biochemistry
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