Author, as appears in the article.: Sunyer-Figueres M; Mas A; Beltran G; Torija MJ

Department: Bioquímica i Biotecnologia

URV's Author/s: Beltran Casellas, Gemma / Mas Baron, Alberto / Torija Martínez, María Jesús

Keywords: Yeast antioxidant response Superoxide dismutase Ros accumula-tion Melatonin supplementation L-tryptophan metabolites Ethanol stress Catalase activity yeast-cells yeast antioxidant response tolerance superoxide dismutase stationary-phase ros accumulation oxidative stress membrane melatonin supplementation growth expression catalase activity alcoholic fermentation adaptive response

Abstract: During alcoholic fermentation, Saccharomyces cerevisiae is subjected to several stresses, among which ethanol is of capital importance. Melatonin, a bioactive molecule synthesized by yeast during alcoholic fermentation, has an antioxidant role and is proposed to contribute to counteract-ing fermentation-associated stresses. The aim of this study was to unravel the protective effect of melatonin on yeast cells subjected to ethanol stress. For that purpose, the effect of ethanol concentrations (6 to 12%) on a wine strain and a lab strain of S. cerevisiae was evaluated, monitoring the viability, growth capacity, mortality, and several indicators of oxidative stress over time, such as reactive oxygen species (ROS) accumulation, lipid peroxidation, and the activity of catalase and superoxide dismutase enzymes. In general, ethanol exposure reduced the cell growth of S. cerevisiae and increased mortality, ROS accumulation, lipid peroxidation and antioxidant enzyme activity. Melatonin supplementation softened the effect of ethanol, enhancing cell growth and decreasing oxidative damage by lowering ROS accumulation, lipid peroxidation, and antioxidant enzyme ac-tivities. However, the effects of melatonin were dependent on strain, melatonin concentration, and growth phase. The results of this study indicate that melatonin has a protective role against mild ethanol stress, mainly by reducing the oxidative stress triggered by this alcohol.

Thematic Areas: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: albert.mas@urv.cat mjesus.torija@urv.cat gemma.beltran@urv.cat

Author identifier: 0000-0002-0763-1679 0000-0001-6419-0745 0000-0002-7071-205X

Record's date: 2023-08-05

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2076-3921/10/11/1735

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Antioxidants. 10 (11):

APA: Sunyer-Figueres M; Mas A; Beltran G; Torija MJ (2021). Protective effects of melatonin on saccharomyces cerevisiae under ethanol stress. Antioxidants, 10(11), -. DOI: 10.3390/antiox10111735

Article's DOI: 10.3390/antiox10111735

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications