Articles producció científica> Bioquímica i Biotecnologia

Looking for a needle in a haystack. SARS-CoV-2 variant characterization in sewage

  • Identification data

    Identifier: imarina:9242253
    Handle: https://hdl.handle.net/20.500.11797/imarina9242253
    Authors:
    Itarte MBofill-Mas SMartínez-Puchol STorrell HCeretó ACarrasco MForés ECanela NGirones RRusiñol M
    Abstract:
    SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective. © 2021 The Authors

  • Others:

    Author, as appears in the article.: Itarte M; Bofill-Mas S; Martínez-Puchol S; Torrell H; Ceretó A; Carrasco M; Forés E; Canela N; Girones R; Rusiñol M
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Cereto Massagué, Adrián José
    Keywords: Wastewater-based epidemiology (wbe) Variants of interest (vois) Variants of concern (vocs) Signature mutations Sars-cov-2 Next-generation sequencing (ngs)
    Abstract: SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective. © 2021 The Authors
    Thematic Areas: Public health, environmental and occupational health Health, toxicology and mutagenesis Environmental chemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: adrianjose.cereto@urv.cat
    Record's date: 2024-07-27
    Papper version: info:eu-repo/semantics/publishedVersion
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Current Opinion In Environmental Science And Health. 24
    APA: Itarte M; Bofill-Mas S; Martínez-Puchol S; Torrell H; Ceretó A; Carrasco M; Forés E; Canela N; Girones R; Rusiñol M (2021). Looking for a needle in a haystack. SARS-CoV-2 variant characterization in sewage. Current Opinion In Environmental Science And Health, 24(), -. DOI: 10.1016/j.coesh.2021.100308
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications

  • Keywords:

    Environmental Chemistry,Environmental Sciences,Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health
    Wastewater-based epidemiology (wbe)
    Variants of interest (vois)
    Variants of concern (vocs)
    Signature mutations
    Sars-cov-2
    Next-generation sequencing (ngs)
    Public health, environmental and occupational health
    Health, toxicology and mutagenesis
    Environmental chemistry

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