Author, as appears in the article.: Itarte M; Bofill-Mas S; Martínez-Puchol S; Torrell H; Ceretó A; Carrasco M; Forés E; Canela N; Girones R; Rusiñol M
Department: Bioquímica i Biotecnologia
URV's Author/s: Cereto Massagué, Adrián José
Keywords: Wastewater-based epidemiology (wbe) Variants of interest (vois) Variants of concern (vocs) Signature mutations Sars-cov-2 Next-generation sequencing (ngs)
Abstract: SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective. © 2021 The Authors
Thematic Areas: Public health, environmental and occupational health Health, toxicology and mutagenesis Environmental chemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: adrianjose.cereto@urv.cat adrianjose.cereto@urv.cat
Record's date: 2023-02-19
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.sciencedirect.com/science/article/pii/S2468584421000805?via%3Dihub
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Current Opinion In Environmental Science And Health. 24
APA: Itarte M; Bofill-Mas S; Martínez-Puchol S; Torrell H; Ceretó A; Carrasco M; Forés E; Canela N; Girones R; Rusiñol M (2021). Looking for a needle in a haystack. SARS-CoV-2 variant characterization in sewage. Current Opinion In Environmental Science And Health, 24(), -. DOI: 10.1016/j.coesh.2021.100308
Article's DOI: 10.1016/j.coesh.2021.100308
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV