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Alterations in metabolome and microbiome associated with an early stress stage in male wistar rats: A multi-omics approach

  • Identification data

    Identifier: imarina:9242266
    Authors:
    Hernandez-Baixauli JPuigbò PAbasolo NPalacios-Jordan HFoguet-Romero ESuñol DGalofré MCaimari ABaselga-Escudero LDel Bas JMMulero M
    Abstract:
    Stress disorders have dramatically increased in recent decades becoming the most prevalent psychiatric disorder in the United States and Europe. However, the diagnosis of stress disorders is currently based on symptom checklist and psychological questionnaires, thus making the identification of candidate biomarkers necessary to gain better insights into this pathology and its related metabolic alterations. Regarding the identification of potential biomarkers, omic profiling and metabolic footprint arise as promising approaches to recognize early biochemical changes in such disease and provide opportunities for the development of integrative candidate biomarkers. Here, we studied plasma and urine metabolites together with metagenomics in a 3 days Chronic Unpredictable Mild Stress (3d CUMS) animal approach that aims to focus on the early stress period of a well-established depression model. The multi-omics integration showed a profile composed by a signature of eight plasma metabolites, six urine metabolites and five microbes. Specifically, threonic acid, malic acid, alpha-ketoglutarate, succinic acid and cholesterol were proposed as key metabolites that could serve as key potential biomarkers in plasma metabolome of early stages of stress. Such findings targeted the threonic acid metabolism and the tricarboxylic acid (TCA) cycle as important pathways in early stress. Additionally, an increase in opportunistic microbes as virus of the Herpesvirales was observed in the microbiota as an effect of the primary stress stages. Our results provide an experimental biochemical characterization of the early stage of CUMS accompanied by a subsequent omic profiling and a metabolic footprinting that provide potential candidate biomarkers. © 2021 by the authors. Licensee MDPI, Basel, S
  • Others:

    Author, as appears in the article.: Hernandez-Baixauli J; Puigbò P; Abasolo N; Palacios-Jordan H; Foguet-Romero E; Suñol D; Galofré M; Caimari A; Baselga-Escudero L; Del Bas JM; Mulero M
    Department: Bioquímica i Biotecnologia
    URV's Author/s: ABASOLO ZABALO, NEREA / BASELGA ESCUDERO, LAURA / Del Bas Prior, José María / Mulero Abellán, Miguel
    Keywords: Unpredictable mild stress Microbiome Metabolome Energy disruption Early stress Chronic unpredictable mild stress Biomarker Animal model proteomics model microbiome metabolome genetics energy disruption depression chronic unpredictable mild stress biomarkers biomarker anxiety animal model
    Abstract: Stress disorders have dramatically increased in recent decades becoming the most prevalent psychiatric disorder in the United States and Europe. However, the diagnosis of stress disorders is currently based on symptom checklist and psychological questionnaires, thus making the identification of candidate biomarkers necessary to gain better insights into this pathology and its related metabolic alterations. Regarding the identification of potential biomarkers, omic profiling and metabolic footprint arise as promising approaches to recognize early biochemical changes in such disease and provide opportunities for the development of integrative candidate biomarkers. Here, we studied plasma and urine metabolites together with metagenomics in a 3 days Chronic Unpredictable Mild Stress (3d CUMS) animal approach that aims to focus on the early stress period of a well-established depression model. The multi-omics integration showed a profile composed by a signature of eight plasma metabolites, six urine metabolites and five microbes. Specifically, threonic acid, malic acid, alpha-ketoglutarate, succinic acid and cholesterol were proposed as key metabolites that could serve as key potential biomarkers in plasma metabolome of early stages of stress. Such findings targeted the threonic acid metabolism and the tricarboxylic acid (TCA) cycle as important pathways in early stress. Additionally, an increase in opportunistic microbes as virus of the Herpesvirales was observed in the microbiota as an effect of the primary stress stages. Our results provide an experimental biochemical characterization of the early stage of CUMS accompanied by a subsequent omic profiling and a metabolic footprinting that provide potential candidate biomarkers. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
    Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: josepm.delbas@urv.cat miquel.mulero@urv.cat
    Record's date: 2024-10-12
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/1422-0067/22/23/12931
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: International Journal Of Molecular Sciences. 22 (23):
    APA: Hernandez-Baixauli J; Puigbò P; Abasolo N; Palacios-Jordan H; Foguet-Romero E; Suñol D; Galofré M; Caimari A; Baselga-Escudero L; Del Bas JM; Mulero M (2021). Alterations in metabolome and microbiome associated with an early stress stage in male wistar rats: A multi-omics approach. International Journal Of Molecular Sciences, 22(23), -. DOI: 10.3390/ijms222312931
    Article's DOI: 10.3390/ijms222312931
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2021
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry & Molecular Biology,Catalysis,Chemistry, Multidisciplinary,Computer Science Applications,Inorganic Chemistry,Medicine (Miscellaneous),Molecular Biology,Organic Chemistry,Physical and Theoretical Chemistry,Spectroscopy
    Unpredictable mild stress
    Microbiome
    Metabolome
    Energy disruption
    Early stress
    Chronic unpredictable mild stress
    Biomarker
    Animal model
    proteomics
    model
    microbiome
    metabolome
    genetics
    energy disruption
    depression
    chronic unpredictable mild stress
    biomarkers
    biomarker
    anxiety
    animal model
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Psicología
    Physical and theoretical chemistry
    Organic chemistry
    Odontología
    Nutrição
    Molecular biology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Inorganic chemistry
    Geociências
    Farmacia
    Engenharias iv
    Engenharias ii
    Engenharias i
    Educação física
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, multidisciplinary
    Catalysis
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Astronomia / física
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